The Effect of Tianmai Xiaoke Pian on Insulin Resistance through PI3-K/AKT Signal Pathway
In the clinical setting, given the potential adverse effects of thiazolidinediones and biguanides, we often have difficulty in treatment that no other insulin sensitizers are available for use in type 2 diabetic mellitus (T2DM) patients. Tianmai Xiaoke Pian (TMXKP) is a traditional Chinese medicine...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2016/9261259 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548930098823168 |
|---|---|
| author | Nana Wang Tiegang Li Ping Han |
| author_facet | Nana Wang Tiegang Li Ping Han |
| author_sort | Nana Wang |
| collection | DOAJ |
| description | In the clinical setting, given the potential adverse effects of thiazolidinediones and biguanides, we often have difficulty in treatment that no other insulin sensitizers are available for use in type 2 diabetic mellitus (T2DM) patients. Tianmai Xiaoke Pian (TMXKP) is a traditional Chinese medicine tablet, which is comprised of chromium picolinate, Tianhuafen, Maidong, and Wuweizi. To understand its mechanism of action on insulin resistance, TMXKP (50 mg/kg orally) was tested in T2DM rats (induced by a high-fat diet and streptozotocin). Eight weeks later, fasting blood glucose (FBG) and oral glucose tolerance tests (OGTT) were performed. Area under the curve (AUC) and homeostatic model assessment of insulin resistance (HOMA-IR) were calculated, and PI3-K/AKT signal pathway-related genes and proteins were tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis in muscle, adipose, and liver tissues, respectively. TMXKP significantly reduced FBG, OGTT, AUC, and HOMA-IR in diabetic rats P<0.05. Furthermore, we also observed that TMXKP could significantly decrease IRS-1, IRS-2, PI3-K p85α, and AKT2 gene expression and also IRS-1, IRS-2, PI3-K, AKT2, and p-AKT2 protein expression levels P<0.05 in diabetic rats. These findings confirm that TMXKP can alleviate insulin resistance in T2DM rats through the PI3K/AKT pathway. Thus TMXKP appears to be a promising insulin sensitizer. |
| format | Article |
| id | doaj-art-fe1d598f240046e1b37ad7d5b0d64d19 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-fe1d598f240046e1b37ad7d5b0d64d192025-02-03T06:12:47ZengWileyJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/92612599261259The Effect of Tianmai Xiaoke Pian on Insulin Resistance through PI3-K/AKT Signal PathwayNana Wang0Tiegang Li1Ping Han2Endocrinology Department, Shengjing Hospital of China Medical University, Shenyang 110004, ChinaEmergency Department, Shengjing Hospital of China Medical University, Shenyang 110004, ChinaEndocrinology Department, Shengjing Hospital of China Medical University, Shenyang 110004, ChinaIn the clinical setting, given the potential adverse effects of thiazolidinediones and biguanides, we often have difficulty in treatment that no other insulin sensitizers are available for use in type 2 diabetic mellitus (T2DM) patients. Tianmai Xiaoke Pian (TMXKP) is a traditional Chinese medicine tablet, which is comprised of chromium picolinate, Tianhuafen, Maidong, and Wuweizi. To understand its mechanism of action on insulin resistance, TMXKP (50 mg/kg orally) was tested in T2DM rats (induced by a high-fat diet and streptozotocin). Eight weeks later, fasting blood glucose (FBG) and oral glucose tolerance tests (OGTT) were performed. Area under the curve (AUC) and homeostatic model assessment of insulin resistance (HOMA-IR) were calculated, and PI3-K/AKT signal pathway-related genes and proteins were tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis in muscle, adipose, and liver tissues, respectively. TMXKP significantly reduced FBG, OGTT, AUC, and HOMA-IR in diabetic rats P<0.05. Furthermore, we also observed that TMXKP could significantly decrease IRS-1, IRS-2, PI3-K p85α, and AKT2 gene expression and also IRS-1, IRS-2, PI3-K, AKT2, and p-AKT2 protein expression levels P<0.05 in diabetic rats. These findings confirm that TMXKP can alleviate insulin resistance in T2DM rats through the PI3K/AKT pathway. Thus TMXKP appears to be a promising insulin sensitizer.http://dx.doi.org/10.1155/2016/9261259 |
| spellingShingle | Nana Wang Tiegang Li Ping Han The Effect of Tianmai Xiaoke Pian on Insulin Resistance through PI3-K/AKT Signal Pathway Journal of Diabetes Research |
| title | The Effect of Tianmai Xiaoke Pian on Insulin Resistance through PI3-K/AKT Signal Pathway |
| title_full | The Effect of Tianmai Xiaoke Pian on Insulin Resistance through PI3-K/AKT Signal Pathway |
| title_fullStr | The Effect of Tianmai Xiaoke Pian on Insulin Resistance through PI3-K/AKT Signal Pathway |
| title_full_unstemmed | The Effect of Tianmai Xiaoke Pian on Insulin Resistance through PI3-K/AKT Signal Pathway |
| title_short | The Effect of Tianmai Xiaoke Pian on Insulin Resistance through PI3-K/AKT Signal Pathway |
| title_sort | effect of tianmai xiaoke pian on insulin resistance through pi3 k akt signal pathway |
| url | http://dx.doi.org/10.1155/2016/9261259 |
| work_keys_str_mv | AT nanawang theeffectoftianmaixiaokepianoninsulinresistancethroughpi3kaktsignalpathway AT tiegangli theeffectoftianmaixiaokepianoninsulinresistancethroughpi3kaktsignalpathway AT pinghan theeffectoftianmaixiaokepianoninsulinresistancethroughpi3kaktsignalpathway AT nanawang effectoftianmaixiaokepianoninsulinresistancethroughpi3kaktsignalpathway AT tiegangli effectoftianmaixiaokepianoninsulinresistancethroughpi3kaktsignalpathway AT pinghan effectoftianmaixiaokepianoninsulinresistancethroughpi3kaktsignalpathway |