Análisis inmunofenotípico de muestras normales de médula ósea: aplicaciones en el control de calidad en los laboratorios de citometría
Immunophenotypic analysis of normal cell samples from bone marrow: applications in quality control of cytometry laboratories.Objective. To describe a standardized flow cytometry protocol for the relative and absolute quantification of hematopoietic cell subpopulationsfrom normal bone marrow, and to...
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Pontificia Universidad Javeriana
2010-12-01
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| Series: | Universitas Scientiarum |
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| Online Access: | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1356/823 |
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| author | Dian Carolina Roa-Higuera Susana Fiorentino Viviana Marcela Rodriguez-Pardo Alba Myriam Campos-Arenas Elvira Antonia Infante-Acosta Claudia Cecilia Cardozo-Romero Sandra Milena Quijano-Gómez |
| author_facet | Dian Carolina Roa-Higuera Susana Fiorentino Viviana Marcela Rodriguez-Pardo Alba Myriam Campos-Arenas Elvira Antonia Infante-Acosta Claudia Cecilia Cardozo-Romero Sandra Milena Quijano-Gómez |
| author_sort | Dian Carolina Roa-Higuera |
| collection | DOAJ |
| description | Immunophenotypic analysis of normal cell samples from bone marrow: applications in quality control of cytometry laboratories.Objective. To describe a standardized flow cytometry protocol for the relative and absolute quantification of hematopoietic cell subpopulationsfrom normal bone marrow, and to evaluate the expression of different lineage-specific cell markers with a reactivity associated to celldifferentiation to be used as part of the routine quality control in cytometry laboratories. Materials and methods. The immunophenotypicalanalysis of different cell subpopulations was done with samples from normal bone marrow using a panel of monoclonal and polyclonalantibodies useful in the characterization of acute leukemias with four different fluorescences, by means of a protocol that combines celllabeling of membrane and cytoplasm antigens. Expression analysis was done in terms of mean fluorescence intensity (MFI). Fluorescentbeads at a known concentration were added for calculating the absolute count of cells. Results. The antibody panel used allowed theidentification and quantification of different normal leukocyte subpopulations of lymphatic and myeloid origin, including CD34+ stem cells and more differentiated cell populations in the granulocytic, monocytic, and erythroid cell lines. We established reference values for cellpopulations and cell marker expression ranges as part of routine quality control of cytometry laboratories. Conclusion. Immunophenotypicpatterns identified as well as absolute and relative reference values for the different normal leukocyte populations from bone marrow can beused by cytometry laboratories as a basis for establishing reference parameters in phenotypic analyses of hematologic neoplasia. |
| format | Article |
| id | doaj-art-fe16dd435c2c42229b5fa528c16e67ff |
| institution | OA Journals |
| issn | 0122-7483 2027-1352 |
| language | English |
| publishDate | 2010-12-01 |
| publisher | Pontificia Universidad Javeriana |
| record_format | Article |
| series | Universitas Scientiarum |
| spelling | doaj-art-fe16dd435c2c42229b5fa528c16e67ff2025-08-20T02:04:40ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522010-12-01153206223Análisis inmunofenotípico de muestras normales de médula ósea: aplicaciones en el control de calidad en los laboratorios de citometríaDian Carolina Roa-HigueraSusana FiorentinoViviana Marcela Rodriguez-PardoAlba Myriam Campos-ArenasElvira Antonia Infante-AcostaClaudia Cecilia Cardozo-RomeroSandra Milena Quijano-GómezImmunophenotypic analysis of normal cell samples from bone marrow: applications in quality control of cytometry laboratories.Objective. To describe a standardized flow cytometry protocol for the relative and absolute quantification of hematopoietic cell subpopulationsfrom normal bone marrow, and to evaluate the expression of different lineage-specific cell markers with a reactivity associated to celldifferentiation to be used as part of the routine quality control in cytometry laboratories. Materials and methods. The immunophenotypicalanalysis of different cell subpopulations was done with samples from normal bone marrow using a panel of monoclonal and polyclonalantibodies useful in the characterization of acute leukemias with four different fluorescences, by means of a protocol that combines celllabeling of membrane and cytoplasm antigens. Expression analysis was done in terms of mean fluorescence intensity (MFI). Fluorescentbeads at a known concentration were added for calculating the absolute count of cells. Results. The antibody panel used allowed theidentification and quantification of different normal leukocyte subpopulations of lymphatic and myeloid origin, including CD34+ stem cells and more differentiated cell populations in the granulocytic, monocytic, and erythroid cell lines. We established reference values for cellpopulations and cell marker expression ranges as part of routine quality control of cytometry laboratories. Conclusion. Immunophenotypicpatterns identified as well as absolute and relative reference values for the different normal leukocyte populations from bone marrow can beused by cytometry laboratories as a basis for establishing reference parameters in phenotypic analyses of hematologic neoplasia.http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1356/823multiparametric flow cytometryimmunophenotypehematologic neoplasianormal bone marrowreference valuesquality control |
| spellingShingle | Dian Carolina Roa-Higuera Susana Fiorentino Viviana Marcela Rodriguez-Pardo Alba Myriam Campos-Arenas Elvira Antonia Infante-Acosta Claudia Cecilia Cardozo-Romero Sandra Milena Quijano-Gómez Análisis inmunofenotípico de muestras normales de médula ósea: aplicaciones en el control de calidad en los laboratorios de citometría Universitas Scientiarum multiparametric flow cytometry immunophenotype hematologic neoplasia normal bone marrow reference values quality control |
| title | Análisis inmunofenotípico de muestras normales de médula ósea: aplicaciones en el control de calidad en los laboratorios de citometría |
| title_full | Análisis inmunofenotípico de muestras normales de médula ósea: aplicaciones en el control de calidad en los laboratorios de citometría |
| title_fullStr | Análisis inmunofenotípico de muestras normales de médula ósea: aplicaciones en el control de calidad en los laboratorios de citometría |
| title_full_unstemmed | Análisis inmunofenotípico de muestras normales de médula ósea: aplicaciones en el control de calidad en los laboratorios de citometría |
| title_short | Análisis inmunofenotípico de muestras normales de médula ósea: aplicaciones en el control de calidad en los laboratorios de citometría |
| title_sort | analisis inmunofenotipico de muestras normales de medula osea aplicaciones en el control de calidad en los laboratorios de citometria |
| topic | multiparametric flow cytometry immunophenotype hematologic neoplasia normal bone marrow reference values quality control |
| url | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1356/823 |
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