Efficacy of disinfectants and endotoxin-retentive filters for the removal of bacterial DNA from dialysates
Abstract Background Bacterial DNA (bDNA) fragments in dialysate lines can trigger inflammatory responses in patients on dialysis. However, no studies have reported the removal and inactivation of bDNA in dialysate lines using cleaning and disinfection, and management procedures to control bDNA conta...
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| Format: | Article |
| Language: | English |
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BMC
2025-05-01
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| Series: | Renal Replacement Therapy |
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| Online Access: | https://doi.org/10.1186/s41100-025-00623-w |
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| author | Minoru Nakamura Ami Murata Toru Yokoyama Daisuke Furuya Tomokazu Indo Shin-ichi Yokota |
| author_facet | Minoru Nakamura Ami Murata Toru Yokoyama Daisuke Furuya Tomokazu Indo Shin-ichi Yokota |
| author_sort | Minoru Nakamura |
| collection | DOAJ |
| description | Abstract Background Bacterial DNA (bDNA) fragments in dialysate lines can trigger inflammatory responses in patients on dialysis. However, no studies have reported the removal and inactivation of bDNA in dialysate lines using cleaning and disinfection, and management procedures to control bDNA contamination have yet to be established. Methods The efficiency of an endotoxin-retentive filter (ETRF) for the removal of bDNA was examined using an experimental dialysate line incorporating an ETRF and the solubilized materials derived from hot-water-disinfected Pseudomonas aeruginosa cells. To examine the inactivation of bDNA by disinfection, P. aeruginosa cell suspensions were disinfected with hot water, peracetic acid, or sodium hypochlorite, and the amount of bDNA remaining after the disinfection treatment was determined. Single-stranded and double-stranded bDNA were measured using Qubit® fluorometry. The molecular size of bDNA was analyzed by polyacrylamide gel electrophoresis. Results In the spike-and-recovery test of solubilized materials derived from hot-water-disinfected bacterial cells, bDNA leakage was observed when the circuit pressure of the inlet ETRF was elevated. bDNA was inactivated more during disinfection with sodium hypochlorite than with peracetic acid and hot water. Conclusions In addition to the ETRF, disinfection with sodium hypochlorite is an effective method for the management of bDNA in dialysates. |
| format | Article |
| id | doaj-art-fe092c13fe9e4f88a53131e5ffb441c3 |
| institution | OA Journals |
| issn | 2059-1381 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
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| series | Renal Replacement Therapy |
| spelling | doaj-art-fe092c13fe9e4f88a53131e5ffb441c32025-08-20T01:49:44ZengBMCRenal Replacement Therapy2059-13812025-05-0111111010.1186/s41100-025-00623-wEfficacy of disinfectants and endotoxin-retentive filters for the removal of bacterial DNA from dialysatesMinoru Nakamura0Ami Murata1Toru Yokoyama2Daisuke Furuya3Tomokazu Indo4Shin-ichi Yokota5Department of Clinical Engineering, Faculty of Health Sciences, Hokkaido University of ScienceDepartment of Medical Technology, Graduate School of Health Sciences, Hokkaido University of ScienceDepartment of Medical Technology, Graduate School of Health Sciences, Hokkaido University of ScienceDepartment of Medical Technology, Graduate School of Health Sciences, Hokkaido University of ScienceDepartment of Medical Technology, Graduate School of Health Sciences, Hokkaido University of ScienceDepartment of Microbiology, Sapporo Medical University School of MedicineAbstract Background Bacterial DNA (bDNA) fragments in dialysate lines can trigger inflammatory responses in patients on dialysis. However, no studies have reported the removal and inactivation of bDNA in dialysate lines using cleaning and disinfection, and management procedures to control bDNA contamination have yet to be established. Methods The efficiency of an endotoxin-retentive filter (ETRF) for the removal of bDNA was examined using an experimental dialysate line incorporating an ETRF and the solubilized materials derived from hot-water-disinfected Pseudomonas aeruginosa cells. To examine the inactivation of bDNA by disinfection, P. aeruginosa cell suspensions were disinfected with hot water, peracetic acid, or sodium hypochlorite, and the amount of bDNA remaining after the disinfection treatment was determined. Single-stranded and double-stranded bDNA were measured using Qubit® fluorometry. The molecular size of bDNA was analyzed by polyacrylamide gel electrophoresis. Results In the spike-and-recovery test of solubilized materials derived from hot-water-disinfected bacterial cells, bDNA leakage was observed when the circuit pressure of the inlet ETRF was elevated. bDNA was inactivated more during disinfection with sodium hypochlorite than with peracetic acid and hot water. Conclusions In addition to the ETRF, disinfection with sodium hypochlorite is an effective method for the management of bDNA in dialysates.https://doi.org/10.1186/s41100-025-00623-wBacterial DNA fragmentsCleaning and disinfectionDialysateHot water disinfectionPeracetic acidSodium hypochlorite |
| spellingShingle | Minoru Nakamura Ami Murata Toru Yokoyama Daisuke Furuya Tomokazu Indo Shin-ichi Yokota Efficacy of disinfectants and endotoxin-retentive filters for the removal of bacterial DNA from dialysates Renal Replacement Therapy Bacterial DNA fragments Cleaning and disinfection Dialysate Hot water disinfection Peracetic acid Sodium hypochlorite |
| title | Efficacy of disinfectants and endotoxin-retentive filters for the removal of bacterial DNA from dialysates |
| title_full | Efficacy of disinfectants and endotoxin-retentive filters for the removal of bacterial DNA from dialysates |
| title_fullStr | Efficacy of disinfectants and endotoxin-retentive filters for the removal of bacterial DNA from dialysates |
| title_full_unstemmed | Efficacy of disinfectants and endotoxin-retentive filters for the removal of bacterial DNA from dialysates |
| title_short | Efficacy of disinfectants and endotoxin-retentive filters for the removal of bacterial DNA from dialysates |
| title_sort | efficacy of disinfectants and endotoxin retentive filters for the removal of bacterial dna from dialysates |
| topic | Bacterial DNA fragments Cleaning and disinfection Dialysate Hot water disinfection Peracetic acid Sodium hypochlorite |
| url | https://doi.org/10.1186/s41100-025-00623-w |
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