Characterization of Synovial Cytokine Patterns in Bucket-Handle and Posterior Horn Meniscal Tears

The specific etiology of meniscal tears, including the mechanism of lesion, location, and orientation, is considered for its contribution to subsequent joint cytokine responsiveness, healing outcomes, and by extension, appropriate lesion-specific surgical remediation. Meniscal repair is desirable to...

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Main Authors: Marco Turati, Davide Maggioni, Nicolò Zanchi, Marta Gandolla, Massimo Gorla, Paola Sacerdote, Silvia Franchi, Laura Rizzi, Alessandra Pedrocchi, Robert J. Omeljaniuk, Giovanni Zatti, Antonio Torsello, Marco Bigoni
Format: Article
Language:English
Published: Wiley 2020-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/2020/5071934
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author Marco Turati
Davide Maggioni
Nicolò Zanchi
Marta Gandolla
Massimo Gorla
Paola Sacerdote
Silvia Franchi
Laura Rizzi
Alessandra Pedrocchi
Robert J. Omeljaniuk
Giovanni Zatti
Antonio Torsello
Marco Bigoni
author_facet Marco Turati
Davide Maggioni
Nicolò Zanchi
Marta Gandolla
Massimo Gorla
Paola Sacerdote
Silvia Franchi
Laura Rizzi
Alessandra Pedrocchi
Robert J. Omeljaniuk
Giovanni Zatti
Antonio Torsello
Marco Bigoni
author_sort Marco Turati
collection DOAJ
description The specific etiology of meniscal tears, including the mechanism of lesion, location, and orientation, is considered for its contribution to subsequent joint cytokine responsiveness, healing outcomes, and by extension, appropriate lesion-specific surgical remediation. Meniscal repair is desirable to reduce the probability of development of posttraumatic osteoarthritis (PTOA) which is strongly influenced by the coordinate generation of pro- and anti-inflammatory cytokines by the injured cartilage. We now present biochemical data on variation in cytokine levels arising from two particular meniscal tears: bucket-handle (BH) and posterior horn (PH) isolated meniscal tears. We selected these two groups due to the different clinical presentations. We measured the concentrations of TNF-α, IL-1β, IL-6, IL-8, and IL-10 in knee synovial fluid of 45 patients with isolated meniscal lesions (BH tear, n=12; PH tear, n=33). TNF-α levels were significantly (p<0.05) greater in the BH group compared with the PH group, whereas IL-1β levels were significantly greater (p<0.05) in the PH group compared with the BH group. Both BH and PH groups were consistent in presenting a positive correlation between concentrations of IL-6 and IL-1β. A fundamental difference in IL-10 responsiveness between the two groups was noted; specifically, levels of IL-10 were positively correlated with IL-6 in the BH group, whereas in the PH group, levels of IL-10 were positively correlated with IL-1β. Collectively, our data suggest a possible influence of the meniscal tear pattern to the articular cytokine responsiveness. This differential expression of inflammatory cytokines may influence the risk of developing PTOA in the long term.
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spelling doaj-art-fe08c655948740109b7c3d89c59cbbb92025-08-20T03:25:47ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/50719345071934Characterization of Synovial Cytokine Patterns in Bucket-Handle and Posterior Horn Meniscal TearsMarco Turati0Davide Maggioni1Nicolò Zanchi2Marta Gandolla3Massimo Gorla4Paola Sacerdote5Silvia Franchi6Laura Rizzi7Alessandra Pedrocchi8Robert J. Omeljaniuk9Giovanni Zatti10Antonio Torsello11Marco Bigoni12Orthopedic Department, San Gerardo Hospital, Monza, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, Monza, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, Monza, ItalyPolitecnico di Milano, NearLab, Department of Electronics, Information and Bioengineering, Milan, ItalyOrthopedic Department, San Gerardo Hospital, Monza, ItalyDepartment of Pharmacological and Biomolecular Sciences, University of Milan, Milan, ItalyDepartment of Pharmacological and Biomolecular Sciences, University of Milan, Milan, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, Monza, ItalyPolitecnico di Milano, NearLab, Department of Electronics, Information and Bioengineering, Milan, ItalyDepartment of Biology, Lakehead University, Thunder Bay, ON, P7B5E1, CanadaOrthopedic Department, San Gerardo Hospital, Monza, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, Monza, ItalyOrthopedic Department, San Gerardo Hospital, Monza, ItalyThe specific etiology of meniscal tears, including the mechanism of lesion, location, and orientation, is considered for its contribution to subsequent joint cytokine responsiveness, healing outcomes, and by extension, appropriate lesion-specific surgical remediation. Meniscal repair is desirable to reduce the probability of development of posttraumatic osteoarthritis (PTOA) which is strongly influenced by the coordinate generation of pro- and anti-inflammatory cytokines by the injured cartilage. We now present biochemical data on variation in cytokine levels arising from two particular meniscal tears: bucket-handle (BH) and posterior horn (PH) isolated meniscal tears. We selected these two groups due to the different clinical presentations. We measured the concentrations of TNF-α, IL-1β, IL-6, IL-8, and IL-10 in knee synovial fluid of 45 patients with isolated meniscal lesions (BH tear, n=12; PH tear, n=33). TNF-α levels were significantly (p<0.05) greater in the BH group compared with the PH group, whereas IL-1β levels were significantly greater (p<0.05) in the PH group compared with the BH group. Both BH and PH groups were consistent in presenting a positive correlation between concentrations of IL-6 and IL-1β. A fundamental difference in IL-10 responsiveness between the two groups was noted; specifically, levels of IL-10 were positively correlated with IL-6 in the BH group, whereas in the PH group, levels of IL-10 were positively correlated with IL-1β. Collectively, our data suggest a possible influence of the meniscal tear pattern to the articular cytokine responsiveness. This differential expression of inflammatory cytokines may influence the risk of developing PTOA in the long term.http://dx.doi.org/10.1155/2020/5071934
spellingShingle Marco Turati
Davide Maggioni
Nicolò Zanchi
Marta Gandolla
Massimo Gorla
Paola Sacerdote
Silvia Franchi
Laura Rizzi
Alessandra Pedrocchi
Robert J. Omeljaniuk
Giovanni Zatti
Antonio Torsello
Marco Bigoni
Characterization of Synovial Cytokine Patterns in Bucket-Handle and Posterior Horn Meniscal Tears
Mediators of Inflammation
title Characterization of Synovial Cytokine Patterns in Bucket-Handle and Posterior Horn Meniscal Tears
title_full Characterization of Synovial Cytokine Patterns in Bucket-Handle and Posterior Horn Meniscal Tears
title_fullStr Characterization of Synovial Cytokine Patterns in Bucket-Handle and Posterior Horn Meniscal Tears
title_full_unstemmed Characterization of Synovial Cytokine Patterns in Bucket-Handle and Posterior Horn Meniscal Tears
title_short Characterization of Synovial Cytokine Patterns in Bucket-Handle and Posterior Horn Meniscal Tears
title_sort characterization of synovial cytokine patterns in bucket handle and posterior horn meniscal tears
url http://dx.doi.org/10.1155/2020/5071934
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