Bacteriocin-Producing Staphylococci and Mammaliicocci Strains for Agro-Food and Public Health Applications with Relevance of Micrococcin P1
Antimicrobial-producing strains and their bacteriocins hold great promise for the control of bacterial diseases, being an attractive alternative to antibiotics. Thus, the aim of this study was to evaluate the inhibitory activity of 15 bacteriocin-producing staphylococci and mammaliicocci (BP-S/M) st...
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2025-01-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/14/1/97 |
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| author | Rosa Fernández-Fernández Carmen Lozano Allelen Campaña-Burguet Carmen González-Azcona Tamara Álvarez-Gómez Rocío Fernández-Pérez Raquel Peña Myriam Zarazaga Jaime Carrasco Carmen Torres |
| author_facet | Rosa Fernández-Fernández Carmen Lozano Allelen Campaña-Burguet Carmen González-Azcona Tamara Álvarez-Gómez Rocío Fernández-Pérez Raquel Peña Myriam Zarazaga Jaime Carrasco Carmen Torres |
| author_sort | Rosa Fernández-Fernández |
| collection | DOAJ |
| description | Antimicrobial-producing strains and their bacteriocins hold great promise for the control of bacterial diseases, being an attractive alternative to antibiotics. Thus, the aim of this study was to evaluate the inhibitory activity of 15 bacteriocin-producing staphylococci and mammaliicocci (BP-S/M) strains and their pre-purified extracts with butanol (BT) against a collection of 27 harmful or zoonotic strains (including Gram-positive/-negative bacteria and molds) with relevance in the public health and agro-food fields. These indicators (excluding Gram-negative strains) were grouped into seven categories based on their potential application areas: dairy livestock mastitis, avian pathogen zoonoses, swine zoonoses, food safety, aquaculture, wine making, and mushroom cultivation. In addition, cross-immunity assays between the BP-S/M strains were carried out to identify potential strain combinations to enhance their activity against pathogens. Finally, the hemolytic and gelatinase activities were tested in the BP-S/M strains. A strong inhibitory capacity of the BP-S/M strains was verified against relevant Gram-positive indicators, such as methicillin-resistant <i>Staphylococcus aureus</i>, <i>Listeria monocytogenes</i>, and <i>Clostridium perfringens</i>, among others, while no activity was detected against Gram-negative ones. Interestingly, several BT extracts inhibited the two mold indicators included in this study as representants of mushroom pathogens. The Micrococcin P1 producer <i>Staphylococcus hominis</i> C5835 (>60% of indicators were intensively inhibited by all the methods) can be proposed as a potential candidate for the control of bacterial diseases in the aforementioned categories alone or in combination with other BP-S/M strains (mainly with <i>Staphylococcus warneri</i> X2969). In this regard, five potential combinations of BP-S/M strains that enhanced their activity against specific pathogens were detected. |
| format | Article |
| id | doaj-art-fdf6ba542a364aa7ae075e6d7f4b1560 |
| institution | Kabale University |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Antibiotics |
| spelling | doaj-art-fdf6ba542a364aa7ae075e6d7f4b15602025-01-24T13:18:59ZengMDPI AGAntibiotics2079-63822025-01-011419710.3390/antibiotics14010097Bacteriocin-Producing Staphylococci and Mammaliicocci Strains for Agro-Food and Public Health Applications with Relevance of Micrococcin P1Rosa Fernández-Fernández0Carmen Lozano1Allelen Campaña-Burguet2Carmen González-Azcona3Tamara Álvarez-Gómez4Rocío Fernández-Pérez5Raquel Peña6Myriam Zarazaga7Jaime Carrasco8Carmen Torres9Area of Biochemistry and Molecular Biology, OneHealth-UR Research Group, University of La Rioja, 26006 Logroño, SpainArea of Biochemistry and Molecular Biology, OneHealth-UR Research Group, University of La Rioja, 26006 Logroño, SpainArea of Biochemistry and Molecular Biology, OneHealth-UR Research Group, University of La Rioja, 26006 Logroño, SpainArea of Biochemistry and Molecular Biology, OneHealth-UR Research Group, University of La Rioja, 26006 Logroño, SpainArea of Biochemistry and Molecular Biology, OneHealth-UR Research Group, University of La Rioja, 26006 Logroño, SpainInstituto de Ciencias de la Vid y del Vino (ICVV) (Universidad de La Rioja, Consejo Superior de Investigaciones Científicas (CSIC), Gobierno de La Rioja), 26007 Logroño, SpainDepartment of Microbiology and Parasitology, Instituto de Investigación Sanitaria de Navarra (IdiSNA), University of Navarra, 31008 Pamplona, SpainArea of Biochemistry and Molecular Biology, OneHealth-UR Research Group, University of La Rioja, 26006 Logroño, SpainDepartment Ecology of Cultivated Mushrooms, Regional Institute for Agri-Food and Forest Research and Development (IRIAF), 16194 Cuenca, SpainArea of Biochemistry and Molecular Biology, OneHealth-UR Research Group, University of La Rioja, 26006 Logroño, SpainAntimicrobial-producing strains and their bacteriocins hold great promise for the control of bacterial diseases, being an attractive alternative to antibiotics. Thus, the aim of this study was to evaluate the inhibitory activity of 15 bacteriocin-producing staphylococci and mammaliicocci (BP-S/M) strains and their pre-purified extracts with butanol (BT) against a collection of 27 harmful or zoonotic strains (including Gram-positive/-negative bacteria and molds) with relevance in the public health and agro-food fields. These indicators (excluding Gram-negative strains) were grouped into seven categories based on their potential application areas: dairy livestock mastitis, avian pathogen zoonoses, swine zoonoses, food safety, aquaculture, wine making, and mushroom cultivation. In addition, cross-immunity assays between the BP-S/M strains were carried out to identify potential strain combinations to enhance their activity against pathogens. Finally, the hemolytic and gelatinase activities were tested in the BP-S/M strains. A strong inhibitory capacity of the BP-S/M strains was verified against relevant Gram-positive indicators, such as methicillin-resistant <i>Staphylococcus aureus</i>, <i>Listeria monocytogenes</i>, and <i>Clostridium perfringens</i>, among others, while no activity was detected against Gram-negative ones. Interestingly, several BT extracts inhibited the two mold indicators included in this study as representants of mushroom pathogens. The Micrococcin P1 producer <i>Staphylococcus hominis</i> C5835 (>60% of indicators were intensively inhibited by all the methods) can be proposed as a potential candidate for the control of bacterial diseases in the aforementioned categories alone or in combination with other BP-S/M strains (mainly with <i>Staphylococcus warneri</i> X2969). In this regard, five potential combinations of BP-S/M strains that enhanced their activity against specific pathogens were detected.https://www.mdpi.com/2079-6382/14/1/97bacteriocinstaphylococcinsmammaliicoccinsbacteriocin-producing strainsantimicrobial activityagro-food |
| spellingShingle | Rosa Fernández-Fernández Carmen Lozano Allelen Campaña-Burguet Carmen González-Azcona Tamara Álvarez-Gómez Rocío Fernández-Pérez Raquel Peña Myriam Zarazaga Jaime Carrasco Carmen Torres Bacteriocin-Producing Staphylococci and Mammaliicocci Strains for Agro-Food and Public Health Applications with Relevance of Micrococcin P1 Antibiotics bacteriocin staphylococcins mammaliicoccins bacteriocin-producing strains antimicrobial activity agro-food |
| title | Bacteriocin-Producing Staphylococci and Mammaliicocci Strains for Agro-Food and Public Health Applications with Relevance of Micrococcin P1 |
| title_full | Bacteriocin-Producing Staphylococci and Mammaliicocci Strains for Agro-Food and Public Health Applications with Relevance of Micrococcin P1 |
| title_fullStr | Bacteriocin-Producing Staphylococci and Mammaliicocci Strains for Agro-Food and Public Health Applications with Relevance of Micrococcin P1 |
| title_full_unstemmed | Bacteriocin-Producing Staphylococci and Mammaliicocci Strains for Agro-Food and Public Health Applications with Relevance of Micrococcin P1 |
| title_short | Bacteriocin-Producing Staphylococci and Mammaliicocci Strains for Agro-Food and Public Health Applications with Relevance of Micrococcin P1 |
| title_sort | bacteriocin producing staphylococci and mammaliicocci strains for agro food and public health applications with relevance of micrococcin p1 |
| topic | bacteriocin staphylococcins mammaliicoccins bacteriocin-producing strains antimicrobial activity agro-food |
| url | https://www.mdpi.com/2079-6382/14/1/97 |
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