Combating oral biofilms in Nigerian schoolchildren: a synergistic approach using Macrosphyra longistyla extracts and titanium-ferrite nanoparticles
Introduction: The burden of infectious and non-infectious debilitating diseases of oral etiology is common in developing countries. The pathogenicity of oral infectious diseases is believed to be exacerbated by the uncontrolled progression of biofilm-producing bacteria. In contemporary research ende...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | deu |
| Published: |
German Medical Science GMS Publishing House
2025-05-01
|
| Series: | GMS Hygiene and Infection Control |
| Subjects: | |
| Online Access: | https://journals.publisso.de/en/journals/hic/volume20/dgkh000551 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849744356238426112 |
|---|---|
| author | Nwankwo, Chukwuemeka E. Osho, Adeleke Adewuy, Adewale Otuechere, Chiagoziem Olawoye, Idowu B. Fayemi, Scott O. Oguzie, Judith U. Uwanibe, Jessica Adesina, Adedotun F. Durugbo, Ernest U. Adedokun, Oluwatobi Ajisegiri, Damilola Akinlawon, Ladimeji Eromon, Philomena Folarin, Onikepe Happi, Christian |
| author_facet | Nwankwo, Chukwuemeka E. Osho, Adeleke Adewuy, Adewale Otuechere, Chiagoziem Olawoye, Idowu B. Fayemi, Scott O. Oguzie, Judith U. Uwanibe, Jessica Adesina, Adedotun F. Durugbo, Ernest U. Adedokun, Oluwatobi Ajisegiri, Damilola Akinlawon, Ladimeji Eromon, Philomena Folarin, Onikepe Happi, Christian |
| author_sort | Nwankwo, Chukwuemeka E. |
| collection | DOAJ |
| description | Introduction: The burden of infectious and non-infectious debilitating diseases of oral etiology is common in developing countries. The pathogenicity of oral infectious diseases is believed to be exacerbated by the uncontrolled progression of biofilm-producing bacteria. In contemporary research endeavours, there is a proposition to utilize anti-infective compounds in the control of biofilm-induced infections. This research was carried out to isolate and control biofilm-producing bacteria using anti-infective nanoparticles and a plant extract.
Methods: Biofilm-producing bacteria were isolated and characterized using microbiological techniques and next-generation sequencing. Antimicrobial susceptibility testing and minimum inhibitory concentration were determined using titanium ferrite (TF) coupled with Macrosphyra longistyla plant extracts. Bioactive antimicrobials were analyzed by Fourier-transform Infrared (FTIR) spectroscopy. The surface morphology was determined using a scanning electron microscope (SEM), and toxicological properties were characterized on adult Wistar rats.
Results: Biofilm-producing bacteria isolated and sequenced in this study are Pseudomonas aeruginosa, Aeromonas caviae, Proteus mirabilis and Serratia marcescens.
The plant extracts coupled with nanoparticles were found to be more bioactive against the biofilm producers than either the plant extracts or the nanoparticles alone. The MICs observed here showed these complexes to be more bioactive against the pathogens in lower concentrations compared to that observed in similar studies.
FTIR revealed that the bands at around 3,000–2,800 cm–1 correspond to C–H stretching vibrations. The bands at around 1,700–1,600 cm–1 corresponded to C=O stretching vibrations. The bands at around 1,500–1,400 cm–1 corresponded to N–H bending vibrations. The presence of these functional groups suggests that Macrosphyra longistyla doped with TF nanoparticles (MSLNP) is a complex compound that contains a variety of different chemical groups.
Histology revealed no significant derangements observed in the histoarchitecture of experimental groups. This suggests that the compound shows potential as antimicrobial therapy in battling bacterial oral biofilms. It is recommended that the compound undergo further testing in the drug design process. |
| format | Article |
| id | doaj-art-fdc254b82958461d8075862ec35b0ebb |
| institution | DOAJ |
| issn | 2196-5226 |
| language | deu |
| publishDate | 2025-05-01 |
| publisher | German Medical Science GMS Publishing House |
| record_format | Article |
| series | GMS Hygiene and Infection Control |
| spelling | doaj-art-fdc254b82958461d8075862ec35b0ebb2025-08-20T03:19:59ZdeuGerman Medical Science GMS Publishing HouseGMS Hygiene and Infection Control2196-52262025-05-0120Doc2210.3205/dgkh000551Combating oral biofilms in Nigerian schoolchildren: a synergistic approach using Macrosphyra longistyla extracts and titanium-ferrite nanoparticlesNwankwo, Chukwuemeka E.0https://orcid.org/0000-0003-0206-8589Osho, Adeleke1Adewuy, Adewale2Otuechere, Chiagoziem3Olawoye, Idowu B.4Fayemi, Scott O.5Oguzie, Judith U.6Uwanibe, Jessica7Adesina, Adedotun F.8Durugbo, Ernest U.9Adedokun, Oluwatobi10Ajisegiri, Damilola11Akinlawon, Ladimeji12Eromon, Philomena13Folarin, Onikepe14Happi, Christian15Department of Biological Sciences, Redeemer’s University, Ede, NigeriaDepartment of Biological Sciences, Redeemer’s University, Ede, NigeriaDepartment of Chemical Sciences, Redeemer’s University, Ede, NigeriaDepartment of Chemical Sciences, Redeemer’s University, Ede, NigeriaDepartment of Microbiology and Immunology, Schulich School of Medicine and Dentistry, Western University, London, Ontario, CanadaDepartment of Biological Sciences and Industrial Biotechnology, Caleb University, Imota, Lagos, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer’s University, Ede, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer’s University, Ede, NigeriaDepartment of Chemical Sciences, Redeemer’s University, Ede, Nigeriaepartment of Biological Sciences, Redeemer’s University, Ede, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer’s University, Ede, NigeriaDepartment of Biological Sciences, Redeemer’s University, Ede, NigeriaDepartment of Chemical Sciences, Redeemer’s University, Ede, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer’s University, Ede, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer’s University, Ede, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer’s University, Ede, NigeriaIntroduction: The burden of infectious and non-infectious debilitating diseases of oral etiology is common in developing countries. The pathogenicity of oral infectious diseases is believed to be exacerbated by the uncontrolled progression of biofilm-producing bacteria. In contemporary research endeavours, there is a proposition to utilize anti-infective compounds in the control of biofilm-induced infections. This research was carried out to isolate and control biofilm-producing bacteria using anti-infective nanoparticles and a plant extract. Methods: Biofilm-producing bacteria were isolated and characterized using microbiological techniques and next-generation sequencing. Antimicrobial susceptibility testing and minimum inhibitory concentration were determined using titanium ferrite (TF) coupled with Macrosphyra longistyla plant extracts. Bioactive antimicrobials were analyzed by Fourier-transform Infrared (FTIR) spectroscopy. The surface morphology was determined using a scanning electron microscope (SEM), and toxicological properties were characterized on adult Wistar rats. Results: Biofilm-producing bacteria isolated and sequenced in this study are Pseudomonas aeruginosa, Aeromonas caviae, Proteus mirabilis and Serratia marcescens. The plant extracts coupled with nanoparticles were found to be more bioactive against the biofilm producers than either the plant extracts or the nanoparticles alone. The MICs observed here showed these complexes to be more bioactive against the pathogens in lower concentrations compared to that observed in similar studies. FTIR revealed that the bands at around 3,000–2,800 cm–1 correspond to C–H stretching vibrations. The bands at around 1,700–1,600 cm–1 corresponded to C=O stretching vibrations. The bands at around 1,500–1,400 cm–1 corresponded to N–H bending vibrations. The presence of these functional groups suggests that Macrosphyra longistyla doped with TF nanoparticles (MSLNP) is a complex compound that contains a variety of different chemical groups. Histology revealed no significant derangements observed in the histoarchitecture of experimental groups. This suggests that the compound shows potential as antimicrobial therapy in battling bacterial oral biofilms. It is recommended that the compound undergo further testing in the drug design process.https://journals.publisso.de/en/journals/hic/volume20/dgkh000551biofilm-producing bacteriapseudomonas aeruginosaaeromonas caviaeproteus mirabilisserratia marcescensmacrosphyra longistyla nanoparticle complexminimum inhibitory concentration |
| spellingShingle | Nwankwo, Chukwuemeka E. Osho, Adeleke Adewuy, Adewale Otuechere, Chiagoziem Olawoye, Idowu B. Fayemi, Scott O. Oguzie, Judith U. Uwanibe, Jessica Adesina, Adedotun F. Durugbo, Ernest U. Adedokun, Oluwatobi Ajisegiri, Damilola Akinlawon, Ladimeji Eromon, Philomena Folarin, Onikepe Happi, Christian Combating oral biofilms in Nigerian schoolchildren: a synergistic approach using Macrosphyra longistyla extracts and titanium-ferrite nanoparticles GMS Hygiene and Infection Control biofilm-producing bacteria pseudomonas aeruginosa aeromonas caviae proteus mirabilis serratia marcescens macrosphyra longistyla nanoparticle complex minimum inhibitory concentration |
| title | Combating oral biofilms in Nigerian schoolchildren: a synergistic approach using Macrosphyra longistyla extracts and titanium-ferrite nanoparticles |
| title_full | Combating oral biofilms in Nigerian schoolchildren: a synergistic approach using Macrosphyra longistyla extracts and titanium-ferrite nanoparticles |
| title_fullStr | Combating oral biofilms in Nigerian schoolchildren: a synergistic approach using Macrosphyra longistyla extracts and titanium-ferrite nanoparticles |
| title_full_unstemmed | Combating oral biofilms in Nigerian schoolchildren: a synergistic approach using Macrosphyra longistyla extracts and titanium-ferrite nanoparticles |
| title_short | Combating oral biofilms in Nigerian schoolchildren: a synergistic approach using Macrosphyra longistyla extracts and titanium-ferrite nanoparticles |
| title_sort | combating oral biofilms in nigerian schoolchildren a synergistic approach using macrosphyra longistyla extracts and titanium ferrite nanoparticles |
| topic | biofilm-producing bacteria pseudomonas aeruginosa aeromonas caviae proteus mirabilis serratia marcescens macrosphyra longistyla nanoparticle complex minimum inhibitory concentration |
| url | https://journals.publisso.de/en/journals/hic/volume20/dgkh000551 |
| work_keys_str_mv | AT nwankwochukwuemekae combatingoralbiofilmsinnigerianschoolchildrenasynergisticapproachusingmacrosphyralongistylaextractsandtitaniumferritenanoparticles AT oshoadeleke combatingoralbiofilmsinnigerianschoolchildrenasynergisticapproachusingmacrosphyralongistylaextractsandtitaniumferritenanoparticles AT adewuyadewale combatingoralbiofilmsinnigerianschoolchildrenasynergisticapproachusingmacrosphyralongistylaextractsandtitaniumferritenanoparticles AT otuecherechiagoziem combatingoralbiofilmsinnigerianschoolchildrenasynergisticapproachusingmacrosphyralongistylaextractsandtitaniumferritenanoparticles AT olawoyeidowub combatingoralbiofilmsinnigerianschoolchildrenasynergisticapproachusingmacrosphyralongistylaextractsandtitaniumferritenanoparticles AT fayemiscotto combatingoralbiofilmsinnigerianschoolchildrenasynergisticapproachusingmacrosphyralongistylaextractsandtitaniumferritenanoparticles AT oguziejudithu combatingoralbiofilmsinnigerianschoolchildrenasynergisticapproachusingmacrosphyralongistylaextractsandtitaniumferritenanoparticles AT uwanibejessica combatingoralbiofilmsinnigerianschoolchildrenasynergisticapproachusingmacrosphyralongistylaextractsandtitaniumferritenanoparticles AT adesinaadedotunf combatingoralbiofilmsinnigerianschoolchildrenasynergisticapproachusingmacrosphyralongistylaextractsandtitaniumferritenanoparticles AT durugboernestu combatingoralbiofilmsinnigerianschoolchildrenasynergisticapproachusingmacrosphyralongistylaextractsandtitaniumferritenanoparticles AT adedokunoluwatobi combatingoralbiofilmsinnigerianschoolchildrenasynergisticapproachusingmacrosphyralongistylaextractsandtitaniumferritenanoparticles AT ajisegiridamilola combatingoralbiofilmsinnigerianschoolchildrenasynergisticapproachusingmacrosphyralongistylaextractsandtitaniumferritenanoparticles AT akinlawonladimeji combatingoralbiofilmsinnigerianschoolchildrenasynergisticapproachusingmacrosphyralongistylaextractsandtitaniumferritenanoparticles AT eromonphilomena combatingoralbiofilmsinnigerianschoolchildrenasynergisticapproachusingmacrosphyralongistylaextractsandtitaniumferritenanoparticles AT folarinonikepe combatingoralbiofilmsinnigerianschoolchildrenasynergisticapproachusingmacrosphyralongistylaextractsandtitaniumferritenanoparticles AT happichristian combatingoralbiofilmsinnigerianschoolchildrenasynergisticapproachusingmacrosphyralongistylaextractsandtitaniumferritenanoparticles |