Identification of Significant Secreted or Membrane-Located Proteins in Laryngeal Squamous Cell Carcinoma
Background. This study is aimed at investigating the expressions and prognostic values of secreted or membrane-located proteins (SMPs) in laryngeal squamous cell carcinoma (LSCC). The correlations between the expressions of SMPs and immune cells’ infiltrations were also investigated. Methods. The ex...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/9089397 |
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| author | Li Yan Chunyan Hu Yangyang Ji Lifen Zou Yang Zhao Yi Zhu Xiaoshen Wang |
| author_facet | Li Yan Chunyan Hu Yangyang Ji Lifen Zou Yang Zhao Yi Zhu Xiaoshen Wang |
| author_sort | Li Yan |
| collection | DOAJ |
| description | Background. This study is aimed at investigating the expressions and prognostic values of secreted or membrane-located proteins (SMPs) in laryngeal squamous cell carcinoma (LSCC). The correlations between the expressions of SMPs and immune cells’ infiltrations were also investigated. Methods. The expression data of normal laryngeal and LSCC samples were obtained from the TCGA and GEO datasets. The differentially expressed SMPs were identified, and their prognostic values were analyzed. The biological functions of differentially expressed and worse-survival-related SMPs were explored. LASSO regression, Cox multivariate analysis, and nomogram were used to construct a model to predict the survival. Then, the infiltrations of the 24 immune cell populations were calculated using the GSVA method, and the correlations between the expression of SMPs and the immune infiltration were investigated. Results. 122 samples (12 normal and 120 LSCC) of the TCGA database and 114 samples (57 normal and 57 LSCC) of GSE127165 were included. We identified that 138 SMPs were significantly upregulated in LSCC samples of both the TCGA and GEO datasets, among which 52 SMPs were significantly correlated with worse survival. GO and KEGG analyses revealed those 52 SMPs significantly participate in tumor microenvironment and immune cells’ communication. Nine of 52 SMPs (ABCC5, ATP1B3, CLEC11A, FLNA, FSTL3, MMP1, NME1, OAS3, and PHLDB2) were included in the nomogram to effectively and accurately predict the LSCC patients’ survival. The expressions of most SMPs, such as MMP1 and FSTL3, were significantly positively correlated with the immune infiltration of LSCC. Conclusions. In this study, the expression, prognostic values, and correlations with immune infiltration of SMPs were analyzed in LSCC samples. Our analyses identified several significant SMPs differentially expressed between normal laryngeal and LSCC samples, correlated with worse survival, and related to the immune infiltration. |
| format | Article |
| id | doaj-art-fdbaf69c67b1473597695e52a3cd1221 |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-fdbaf69c67b1473597695e52a3cd12212025-08-20T03:25:27ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/9089397Identification of Significant Secreted or Membrane-Located Proteins in Laryngeal Squamous Cell CarcinomaLi Yan0Chunyan Hu1Yangyang Ji2Lifen Zou3Yang Zhao4Yi Zhu5Xiaoshen Wang6Department of Radiation OncologyDepartment of PathologyDepartment of OtolaryngologyDepartment of Radiation OncologyDepartment of Radiation OncologyDepartment of Radiation OncologyDepartment of Radiation OncologyBackground. This study is aimed at investigating the expressions and prognostic values of secreted or membrane-located proteins (SMPs) in laryngeal squamous cell carcinoma (LSCC). The correlations between the expressions of SMPs and immune cells’ infiltrations were also investigated. Methods. The expression data of normal laryngeal and LSCC samples were obtained from the TCGA and GEO datasets. The differentially expressed SMPs were identified, and their prognostic values were analyzed. The biological functions of differentially expressed and worse-survival-related SMPs were explored. LASSO regression, Cox multivariate analysis, and nomogram were used to construct a model to predict the survival. Then, the infiltrations of the 24 immune cell populations were calculated using the GSVA method, and the correlations between the expression of SMPs and the immune infiltration were investigated. Results. 122 samples (12 normal and 120 LSCC) of the TCGA database and 114 samples (57 normal and 57 LSCC) of GSE127165 were included. We identified that 138 SMPs were significantly upregulated in LSCC samples of both the TCGA and GEO datasets, among which 52 SMPs were significantly correlated with worse survival. GO and KEGG analyses revealed those 52 SMPs significantly participate in tumor microenvironment and immune cells’ communication. Nine of 52 SMPs (ABCC5, ATP1B3, CLEC11A, FLNA, FSTL3, MMP1, NME1, OAS3, and PHLDB2) were included in the nomogram to effectively and accurately predict the LSCC patients’ survival. The expressions of most SMPs, such as MMP1 and FSTL3, were significantly positively correlated with the immune infiltration of LSCC. Conclusions. In this study, the expression, prognostic values, and correlations with immune infiltration of SMPs were analyzed in LSCC samples. Our analyses identified several significant SMPs differentially expressed between normal laryngeal and LSCC samples, correlated with worse survival, and related to the immune infiltration.http://dx.doi.org/10.1155/2022/9089397 |
| spellingShingle | Li Yan Chunyan Hu Yangyang Ji Lifen Zou Yang Zhao Yi Zhu Xiaoshen Wang Identification of Significant Secreted or Membrane-Located Proteins in Laryngeal Squamous Cell Carcinoma Journal of Immunology Research |
| title | Identification of Significant Secreted or Membrane-Located Proteins in Laryngeal Squamous Cell Carcinoma |
| title_full | Identification of Significant Secreted or Membrane-Located Proteins in Laryngeal Squamous Cell Carcinoma |
| title_fullStr | Identification of Significant Secreted or Membrane-Located Proteins in Laryngeal Squamous Cell Carcinoma |
| title_full_unstemmed | Identification of Significant Secreted or Membrane-Located Proteins in Laryngeal Squamous Cell Carcinoma |
| title_short | Identification of Significant Secreted or Membrane-Located Proteins in Laryngeal Squamous Cell Carcinoma |
| title_sort | identification of significant secreted or membrane located proteins in laryngeal squamous cell carcinoma |
| url | http://dx.doi.org/10.1155/2022/9089397 |
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