Dysregulation of Ephrin receptor and PPAR signaling pathways in neural progenitor cells infected by Zika virus
Zika virus (ZIKV) infection is a serious public threat with cases reported in about 70 countries and territories. One of the most serious consequences of ZIKV infection is congenital microcephaly in babies. Congenital microcephaly has been suggested to result from infection of neural progenitor cell...
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1818631 |
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| author | Sathya N. Thulasi Raman Elyse Latreille Jun Gao Wanyue Zhang Jianguo Wu Marsha S. Russell Lisa Walrond Terry Cyr Jessie R. Lavoie David Safronetz Jingxin Cao Simon Sauve Aaron Farnsworth Wangxue Chen Pei-Yong Shi Youchun Wang Lisheng Wang Michael Rosu-Myles Xuguang Li |
| author_facet | Sathya N. Thulasi Raman Elyse Latreille Jun Gao Wanyue Zhang Jianguo Wu Marsha S. Russell Lisa Walrond Terry Cyr Jessie R. Lavoie David Safronetz Jingxin Cao Simon Sauve Aaron Farnsworth Wangxue Chen Pei-Yong Shi Youchun Wang Lisheng Wang Michael Rosu-Myles Xuguang Li |
| author_sort | Sathya N. Thulasi Raman |
| collection | DOAJ |
| description | Zika virus (ZIKV) infection is a serious public threat with cases reported in about 70 countries and territories. One of the most serious consequences of ZIKV infection is congenital microcephaly in babies. Congenital microcephaly has been suggested to result from infection of neural progenitor cells (NPCs) in the developing fetal brain. However, the molecular and cellular mechanisms underlying microcephaly development remains to be fully elucidated. In this study, we employed quantitative proteomics to determine protein expression profile that occur during viral replication in NPCs. Bioinformatics analysis of the protein expression changes resulted in the identification of a wide range of cell signaling pathways. Specifically, pathways involved in neurogenesis and embryonic development were markedly altered, along with those associated with cell cycle, apoptosis, lipid metabolism and oxidative stress. Notably, the differential regulation of Ephrin Receptor and PPAR signaling pathways, as revealed by quantitative proteomics and validated by qPCR array, underscores the need to explore these pathways in disease development. Collectively, these results indicate that ZIKV-induced pathogenesis involves complex virus-host reactions; the findings reported here could help shed light on the mechanisms underlying ZIKV-induced microcephaly and ZIKV replication in NPCs. |
| format | Article |
| id | doaj-art-fdad955415bd458c92d94ba2bdcf4fe1 |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-fdad955415bd458c92d94ba2bdcf4fe12025-08-20T02:16:09ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-01912046206010.1080/22221751.2020.1818631Dysregulation of Ephrin receptor and PPAR signaling pathways in neural progenitor cells infected by Zika virusSathya N. Thulasi Raman0Elyse Latreille1Jun Gao2Wanyue Zhang3Jianguo Wu4Marsha S. Russell5Lisa Walrond6Terry Cyr7Jessie R. Lavoie8David Safronetz9Jingxin Cao10Simon Sauve11Aaron Farnsworth12Wangxue Chen13Pei-Yong Shi14Youchun Wang15Lisheng Wang16Michael Rosu-Myles17Xuguang Li18Centre for Biologics Evaluation, Biologics and Radiopharmaceutical Drugs Directorate, HPFB, Health Canada and WHO Collaborating Centre for Standardization and Evaluation of Biologicals, Ottawa, ON, CanadaCentre for Biologics Evaluation, Biologics and Radiopharmaceutical Drugs Directorate, HPFB, Health Canada and WHO Collaborating Centre for Standardization and Evaluation of Biologicals, Ottawa, ON, CanadaCentre for Biologics Evaluation, Biologics and Radiopharmaceutical Drugs Directorate, HPFB, Health Canada and WHO Collaborating Centre for Standardization and Evaluation of Biologicals, Ottawa, ON, CanadaCentre for Biologics Evaluation, Biologics and Radiopharmaceutical Drugs Directorate, HPFB, Health Canada and WHO Collaborating Centre for Standardization and Evaluation of Biologicals, Ottawa, ON, CanadaCentre for Biologics Evaluation, Biologics and Radiopharmaceutical Drugs Directorate, HPFB, Health Canada and WHO Collaborating Centre for Standardization and Evaluation of Biologicals, Ottawa, ON, CanadaCentre for Biologics Evaluation, Biologics and Radiopharmaceutical Drugs Directorate, HPFB, Health Canada and WHO Collaborating Centre for Standardization and Evaluation of Biologicals, Ottawa, ON, CanadaCentre for Biologics Evaluation, Biologics and Radiopharmaceutical Drugs Directorate, HPFB, Health Canada and WHO Collaborating Centre for Standardization and Evaluation of Biologicals, Ottawa, ON, CanadaCentre for Biologics Evaluation, Biologics and Radiopharmaceutical Drugs Directorate, HPFB, Health Canada and WHO Collaborating Centre for Standardization and Evaluation of Biologicals, Ottawa, ON, CanadaCentre for Biologics Evaluation, Biologics and Radiopharmaceutical Drugs Directorate, HPFB, Health Canada and WHO Collaborating Centre for Standardization and Evaluation of Biologicals, Ottawa, ON, CanadaNational Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, CanadaNational Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, CanadaCentre for Biologics Evaluation, Biologics and Radiopharmaceutical Drugs Directorate, HPFB, Health Canada and WHO Collaborating Centre for Standardization and Evaluation of Biologicals, Ottawa, ON, CanadaCentre for Biologics Evaluation, Biologics and Radiopharmaceutical Drugs Directorate, HPFB, Health Canada and WHO Collaborating Centre for Standardization and Evaluation of Biologicals, Ottawa, ON, CanadaNational Research Council of Canada, Human Health Therapeutics, Ottawa, ON, CanadaDepartment of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX, USANational Institute for Food and Drug Control and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, People’s Republic of ChinaDepartment of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, CanadaCentre for Biologics Evaluation, Biologics and Radiopharmaceutical Drugs Directorate, HPFB, Health Canada and WHO Collaborating Centre for Standardization and Evaluation of Biologicals, Ottawa, ON, CanadaCentre for Biologics Evaluation, Biologics and Radiopharmaceutical Drugs Directorate, HPFB, Health Canada and WHO Collaborating Centre for Standardization and Evaluation of Biologicals, Ottawa, ON, CanadaZika virus (ZIKV) infection is a serious public threat with cases reported in about 70 countries and territories. One of the most serious consequences of ZIKV infection is congenital microcephaly in babies. Congenital microcephaly has been suggested to result from infection of neural progenitor cells (NPCs) in the developing fetal brain. However, the molecular and cellular mechanisms underlying microcephaly development remains to be fully elucidated. In this study, we employed quantitative proteomics to determine protein expression profile that occur during viral replication in NPCs. Bioinformatics analysis of the protein expression changes resulted in the identification of a wide range of cell signaling pathways. Specifically, pathways involved in neurogenesis and embryonic development were markedly altered, along with those associated with cell cycle, apoptosis, lipid metabolism and oxidative stress. Notably, the differential regulation of Ephrin Receptor and PPAR signaling pathways, as revealed by quantitative proteomics and validated by qPCR array, underscores the need to explore these pathways in disease development. Collectively, these results indicate that ZIKV-induced pathogenesis involves complex virus-host reactions; the findings reported here could help shed light on the mechanisms underlying ZIKV-induced microcephaly and ZIKV replication in NPCs.https://www.tandfonline.com/doi/10.1080/22221751.2020.1818631Neural progenitor cellsZIKVEphrin signalingPPAR signalingproteomicsingenuity pathway analysis |
| spellingShingle | Sathya N. Thulasi Raman Elyse Latreille Jun Gao Wanyue Zhang Jianguo Wu Marsha S. Russell Lisa Walrond Terry Cyr Jessie R. Lavoie David Safronetz Jingxin Cao Simon Sauve Aaron Farnsworth Wangxue Chen Pei-Yong Shi Youchun Wang Lisheng Wang Michael Rosu-Myles Xuguang Li Dysregulation of Ephrin receptor and PPAR signaling pathways in neural progenitor cells infected by Zika virus Emerging Microbes and Infections Neural progenitor cells ZIKV Ephrin signaling PPAR signaling proteomics ingenuity pathway analysis |
| title | Dysregulation of Ephrin receptor and PPAR signaling pathways in neural progenitor cells infected by Zika virus |
| title_full | Dysregulation of Ephrin receptor and PPAR signaling pathways in neural progenitor cells infected by Zika virus |
| title_fullStr | Dysregulation of Ephrin receptor and PPAR signaling pathways in neural progenitor cells infected by Zika virus |
| title_full_unstemmed | Dysregulation of Ephrin receptor and PPAR signaling pathways in neural progenitor cells infected by Zika virus |
| title_short | Dysregulation of Ephrin receptor and PPAR signaling pathways in neural progenitor cells infected by Zika virus |
| title_sort | dysregulation of ephrin receptor and ppar signaling pathways in neural progenitor cells infected by zika virus |
| topic | Neural progenitor cells ZIKV Ephrin signaling PPAR signaling proteomics ingenuity pathway analysis |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1818631 |
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