Identification of sex-linked markers and genes in Portuguese oyster (Magallana angulata)
The Portuguese oyster (Magallana angulata) is a valuable aquaculture species with sexual dimorphism in growth and nutritional traits, making it a promising candidate for sex-controlled breeding. However, the genetic mechanisms underlying its sex determination remain poorly understood. This study rep...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2025.1542815/full |
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| author | Xiaoyan Zhou Yue Ning Jingyi Xie Yi Han Chaonan Tang Caiyuan Su Qijuan Wan Qisheng Wu Xiang Guo Jianfei Qi Yizhou Ke Hui Ge Mingyi Cai |
| author_facet | Xiaoyan Zhou Yue Ning Jingyi Xie Yi Han Chaonan Tang Caiyuan Su Qijuan Wan Qisheng Wu Xiang Guo Jianfei Qi Yizhou Ke Hui Ge Mingyi Cai |
| author_sort | Xiaoyan Zhou |
| collection | DOAJ |
| description | The Portuguese oyster (Magallana angulata) is a valuable aquaculture species with sexual dimorphism in growth and nutritional traits, making it a promising candidate for sex-controlled breeding. However, the genetic mechanisms underlying its sex determination remain poorly understood. This study represents the first population-level investigation to identify sex-linked genetic markers and candidate genes in M. angulata using genome-wide association studies (GWAS) and transcriptomic analysis. A total of 1,613 sex-associated SNPs were identified, primarily located on Chr. 9 (7.99 Mb–10.51Mb). SNP annotation revealed 57 genes around these sex-associated SNPs. Transcriptomic analysis identified 8,635 differentially expressed genes (DEGs) between male and female gonads, of which 12 overlapped with GWAS-identified genes. Among these, four genes (5-HTR, ADPKD1-a, SCP, and SLC28A3) exhibited strong sex-specific genetic differentiation, with 5-HTR showing the most pronounced association. Further population genetic analysis revealed higher genetic diversity and heterozygosity of 5-HTR in males. Six sex-associated haplotypes based on SNPs within 5-HTR were constructed and validated in an independent population, although with reduced significance, suggesting both continuity and variation in sex determination mechanisms across populations. These findings provide a critical foundation for understanding the genetic basis of sex determination and advancing sex-controlled breeding strategies in M. angulata. |
| format | Article |
| id | doaj-art-fdac502b0bef4c76b0d887f46e4f1591 |
| institution | Kabale University |
| issn | 2296-7745 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj-art-fdac502b0bef4c76b0d887f46e4f15912025-08-20T03:27:29ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452025-06-011210.3389/fmars.2025.15428151542815Identification of sex-linked markers and genes in Portuguese oyster (Magallana angulata)Xiaoyan Zhou0Yue Ning1Jingyi Xie2Yi Han3Chaonan Tang4Caiyuan Su5Qijuan Wan6Qisheng Wu7Xiang Guo8Jianfei Qi9Yizhou Ke10Hui Ge11Mingyi Cai12Fisheries College, Jimei University; Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural, Xiamen, Fujian, ChinaFisheries Research Institute of Fujian, Key laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian, Xiamen, ChinaFisheries College, Jimei University; Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural, Xiamen, Fujian, ChinaFisheries College, Jimei University; Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural, Xiamen, Fujian, ChinaFisheries College, Jimei University; Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural, Xiamen, Fujian, ChinaFisheries College, Jimei University; Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural, Xiamen, Fujian, ChinaFisheries College, Jimei University; Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural, Xiamen, Fujian, ChinaFisheries Research Institute of Fujian, Key laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian, Xiamen, ChinaFisheries Research Institute of Fujian, Key laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian, Xiamen, ChinaFisheries Research Institute of Fujian, Key laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian, Xiamen, ChinaFisheries College, Jimei University; Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural, Xiamen, Fujian, ChinaFisheries Research Institute of Fujian, Key laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian, Xiamen, ChinaFisheries College, Jimei University; Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural, Xiamen, Fujian, ChinaThe Portuguese oyster (Magallana angulata) is a valuable aquaculture species with sexual dimorphism in growth and nutritional traits, making it a promising candidate for sex-controlled breeding. However, the genetic mechanisms underlying its sex determination remain poorly understood. This study represents the first population-level investigation to identify sex-linked genetic markers and candidate genes in M. angulata using genome-wide association studies (GWAS) and transcriptomic analysis. A total of 1,613 sex-associated SNPs were identified, primarily located on Chr. 9 (7.99 Mb–10.51Mb). SNP annotation revealed 57 genes around these sex-associated SNPs. Transcriptomic analysis identified 8,635 differentially expressed genes (DEGs) between male and female gonads, of which 12 overlapped with GWAS-identified genes. Among these, four genes (5-HTR, ADPKD1-a, SCP, and SLC28A3) exhibited strong sex-specific genetic differentiation, with 5-HTR showing the most pronounced association. Further population genetic analysis revealed higher genetic diversity and heterozygosity of 5-HTR in males. Six sex-associated haplotypes based on SNPs within 5-HTR were constructed and validated in an independent population, although with reduced significance, suggesting both continuity and variation in sex determination mechanisms across populations. These findings provide a critical foundation for understanding the genetic basis of sex determination and advancing sex-controlled breeding strategies in M. angulata.https://www.frontiersin.org/articles/10.3389/fmars.2025.1542815/fullMagallana angulatasex-linked markersgenome-wide association analysistranscriptomic analysissex determination |
| spellingShingle | Xiaoyan Zhou Yue Ning Jingyi Xie Yi Han Chaonan Tang Caiyuan Su Qijuan Wan Qisheng Wu Xiang Guo Jianfei Qi Yizhou Ke Hui Ge Mingyi Cai Identification of sex-linked markers and genes in Portuguese oyster (Magallana angulata) Frontiers in Marine Science Magallana angulata sex-linked markers genome-wide association analysis transcriptomic analysis sex determination |
| title | Identification of sex-linked markers and genes in Portuguese oyster (Magallana angulata) |
| title_full | Identification of sex-linked markers and genes in Portuguese oyster (Magallana angulata) |
| title_fullStr | Identification of sex-linked markers and genes in Portuguese oyster (Magallana angulata) |
| title_full_unstemmed | Identification of sex-linked markers and genes in Portuguese oyster (Magallana angulata) |
| title_short | Identification of sex-linked markers and genes in Portuguese oyster (Magallana angulata) |
| title_sort | identification of sex linked markers and genes in portuguese oyster magallana angulata |
| topic | Magallana angulata sex-linked markers genome-wide association analysis transcriptomic analysis sex determination |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2025.1542815/full |
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