Tumor-Infiltrating Lymphocytes and Neutrophils and Their Location in Canine Mammary Neoplasms with a Solid Arrangement: A Prognostic Factor?
In canine mammary neoplasms, greater inflammation is associated with higher histological grade, lymphatic invasion, and metastases. This retrospective study assessed the density of peri- and intratumoral tumor-infiltrating lymphocytes (TILs), tumor-associated neutrophils (TANs), and CD3<sup>+&...
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2025-01-01
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| author | Mayra C. Flecher Marina P. Reys Débora Balabram Karen Y. R. Nakagaki Geovanni D. Cassali |
| author_facet | Mayra C. Flecher Marina P. Reys Débora Balabram Karen Y. R. Nakagaki Geovanni D. Cassali |
| author_sort | Mayra C. Flecher |
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| description | In canine mammary neoplasms, greater inflammation is associated with higher histological grade, lymphatic invasion, and metastases. This retrospective study assessed the density of peri- and intratumoral tumor-infiltrating lymphocytes (TILs), tumor-associated neutrophils (TANs), and CD3<sup>+</sup> and CD79<sup>+</sup> lymphocytes in canine mammary neoplasms with a solid arrangement, and associated such data with histological types, immunophenotype, prognostic factors, cyclooxygenase-2 (Cox-2) expression and overall and cancer-specific survival. Sixty-one neoplasms with a solid arrangement were classified as malignant myoepitheliomas (6/9.8%), solid papillary carcinomas (8/13.1%), carcinomas with a solid pattern (9/14.8%), basaloid carcinomas (BC) (19/31.1%), and malignant adenomyoepitheliomas (19/31.1%). Intra- and peritumoral TILs, TANs, and TCD3<sup>+</sup> and BCD79<sup>+</sup> lymphocytes were counted, and based on the resulting median, the neoplasms were divided into low or high cell infiltration. BCs had the lowest density of intratumoral TILs (<i>p</i> = 0.02), and luminal B neoplasms showed a significantly higher density of intratumoral TCD3<sup>+</sup> than luminal A cases. Neoplasms with a higher density of peritumoral CD3<sup>+</sup> and CD79<sup>+</sup> had significantly greater proliferative activity. High infiltration of intratumoral BCD79<sup>+</sup> lymphocytes was related to nodal metastasis (<i>p</i> = 0.03). Intratumoral TILs and TCD3<sup>+</sup> were associated with shorter survival time. Therefore, intratumoral lymphocyte infiltration is possibly an important feature in the progression of cancer and influences the survival in bitches with solid arrangement neoplasms. |
| format | Article |
| id | doaj-art-fd93a6e0916f463882c4a460cda4b168 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-fd93a6e0916f463882c4a460cda4b1682025-01-24T13:18:27ZengMDPI AGAnimals2076-26152025-01-0115228710.3390/ani15020287Tumor-Infiltrating Lymphocytes and Neutrophils and Their Location in Canine Mammary Neoplasms with a Solid Arrangement: A Prognostic Factor?Mayra C. Flecher0Marina P. Reys1Débora Balabram2Karen Y. R. Nakagaki3Geovanni D. Cassali4Department of General Pathology, Laboratory of Comparative Pathology, Federal University of Minas Gerais, Belo Horizonte 31270-091, Minas Gerais, BrazilDepartment of General Pathology, Laboratory of Comparative Pathology, Federal University of Minas Gerais, Belo Horizonte 31270-091, Minas Gerais, BrazilDepartment of Surgery, School of Medicine, Federal University of Minas Gerais, Belo Horizonte 31270-091, Minas Gerais, BrazilDepartment of General Pathology, Laboratory of Comparative Pathology, Federal University of Minas Gerais, Belo Horizonte 31270-091, Minas Gerais, BrazilDepartment of General Pathology, Laboratory of Comparative Pathology, Federal University of Minas Gerais, Belo Horizonte 31270-091, Minas Gerais, BrazilIn canine mammary neoplasms, greater inflammation is associated with higher histological grade, lymphatic invasion, and metastases. This retrospective study assessed the density of peri- and intratumoral tumor-infiltrating lymphocytes (TILs), tumor-associated neutrophils (TANs), and CD3<sup>+</sup> and CD79<sup>+</sup> lymphocytes in canine mammary neoplasms with a solid arrangement, and associated such data with histological types, immunophenotype, prognostic factors, cyclooxygenase-2 (Cox-2) expression and overall and cancer-specific survival. Sixty-one neoplasms with a solid arrangement were classified as malignant myoepitheliomas (6/9.8%), solid papillary carcinomas (8/13.1%), carcinomas with a solid pattern (9/14.8%), basaloid carcinomas (BC) (19/31.1%), and malignant adenomyoepitheliomas (19/31.1%). Intra- and peritumoral TILs, TANs, and TCD3<sup>+</sup> and BCD79<sup>+</sup> lymphocytes were counted, and based on the resulting median, the neoplasms were divided into low or high cell infiltration. BCs had the lowest density of intratumoral TILs (<i>p</i> = 0.02), and luminal B neoplasms showed a significantly higher density of intratumoral TCD3<sup>+</sup> than luminal A cases. Neoplasms with a higher density of peritumoral CD3<sup>+</sup> and CD79<sup>+</sup> had significantly greater proliferative activity. High infiltration of intratumoral BCD79<sup>+</sup> lymphocytes was related to nodal metastasis (<i>p</i> = 0.03). Intratumoral TILs and TCD3<sup>+</sup> were associated with shorter survival time. Therefore, intratumoral lymphocyte infiltration is possibly an important feature in the progression of cancer and influences the survival in bitches with solid arrangement neoplasms.https://www.mdpi.com/2076-2615/15/2/287mammary neoplasmstumor-infiltrating lymphocytessurvival analysisCD3CD79neutrophils |
| spellingShingle | Mayra C. Flecher Marina P. Reys Débora Balabram Karen Y. R. Nakagaki Geovanni D. Cassali Tumor-Infiltrating Lymphocytes and Neutrophils and Their Location in Canine Mammary Neoplasms with a Solid Arrangement: A Prognostic Factor? Animals mammary neoplasms tumor-infiltrating lymphocytes survival analysis CD3 CD79 neutrophils |
| title | Tumor-Infiltrating Lymphocytes and Neutrophils and Their Location in Canine Mammary Neoplasms with a Solid Arrangement: A Prognostic Factor? |
| title_full | Tumor-Infiltrating Lymphocytes and Neutrophils and Their Location in Canine Mammary Neoplasms with a Solid Arrangement: A Prognostic Factor? |
| title_fullStr | Tumor-Infiltrating Lymphocytes and Neutrophils and Their Location in Canine Mammary Neoplasms with a Solid Arrangement: A Prognostic Factor? |
| title_full_unstemmed | Tumor-Infiltrating Lymphocytes and Neutrophils and Their Location in Canine Mammary Neoplasms with a Solid Arrangement: A Prognostic Factor? |
| title_short | Tumor-Infiltrating Lymphocytes and Neutrophils and Their Location in Canine Mammary Neoplasms with a Solid Arrangement: A Prognostic Factor? |
| title_sort | tumor infiltrating lymphocytes and neutrophils and their location in canine mammary neoplasms with a solid arrangement a prognostic factor |
| topic | mammary neoplasms tumor-infiltrating lymphocytes survival analysis CD3 CD79 neutrophils |
| url | https://www.mdpi.com/2076-2615/15/2/287 |
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