The role of bioactive compounds in coconut water-herb fermentation: formation of biological nanoparticles and their application in the modulation of intestinal microbiota

This research investigates how bioactive compounds in fermented coconut water with herbal rhizomes (ginger, turmeric, lemongrass) contribute to biological nanoparticle formation and intestinal microbiota modulation. The study employed probiotic cultures to ferment the coconut water-herbal rhizome mi...

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Main Authors: Sofi Nabila, Arie Srihardyastutie, Aulanni’am, Tri Ardiyati
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:CyTA - Journal of Food
Subjects:
Online Access:https://www.tandfonline.com/doi/10.1080/19476337.2025.2542528
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author Sofi Nabila
Arie Srihardyastutie
Aulanni’am
Tri Ardiyati
author_facet Sofi Nabila
Arie Srihardyastutie
Aulanni’am
Tri Ardiyati
author_sort Sofi Nabila
collection DOAJ
description This research investigates how bioactive compounds in fermented coconut water with herbal rhizomes (ginger, turmeric, lemongrass) contribute to biological nanoparticle formation and intestinal microbiota modulation. The study employed probiotic cultures to ferment the coconut water-herbal rhizome mixture and conducted comprehensive analyses including 16S rRNA gene sequencing, antioxidant activity assessment, antibacterial testing against E. coli, metabolite profiling via LC-HRMS/MS, and particle size characterization. Results revealed Limosilactobacillus dominance (80.2 ± 2.1%) in the fermentation process, with peak antioxidant activity occurring on day 3 (6.0 ± 0.3-fold increase,p < .001). The optimal antibacterial activity against E. coli was achieved with equal ratios (1:1:1) of herbal rhizomes. Metabolite analysis identified bioactive compounds including chlorogenic acid (2.3-fold increase), curcumin (4.1-fold increase), and gingerol (3.2-fold increase). Notably, fermentation reduced particle sizes to nanoscale (100–300 nm vs 1000–2000 nm in controls), enhancing bioactive compound bioavailability through increase surface area effects. The findings suggest that this fermented product has significant potential as a nutraceutical for intestinal microbiota modulation and immune system enhancement.
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publishDate 2025-12-01
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series CyTA - Journal of Food
spelling doaj-art-fd92b4cf526c454b8edac814788d845c2025-08-20T02:55:09ZengTaylor & Francis GroupCyTA - Journal of Food1947-63371947-63452025-12-0123110.1080/19476337.2025.2542528The role of bioactive compounds in coconut water-herb fermentation: formation of biological nanoparticles and their application in the modulation of intestinal microbiotaSofi Nabila0Arie Srihardyastutie1Aulanni’am2Tri Ardiyati3Chemistry Department, Faculty of Mathematics and Natural Science, Brawijaya University, Malang, IndonesiaChemistry Department, Faculty of Mathematics and Natural Science, Brawijaya University, Malang, IndonesiaChemistry Department, Faculty of Mathematics and Natural Science, Brawijaya University, Malang, IndonesiaBiology Department, Faculty of Mathematics and Natural Science, Brawijaya University, Malang, IndonesiaThis research investigates how bioactive compounds in fermented coconut water with herbal rhizomes (ginger, turmeric, lemongrass) contribute to biological nanoparticle formation and intestinal microbiota modulation. The study employed probiotic cultures to ferment the coconut water-herbal rhizome mixture and conducted comprehensive analyses including 16S rRNA gene sequencing, antioxidant activity assessment, antibacterial testing against E. coli, metabolite profiling via LC-HRMS/MS, and particle size characterization. Results revealed Limosilactobacillus dominance (80.2 ± 2.1%) in the fermentation process, with peak antioxidant activity occurring on day 3 (6.0 ± 0.3-fold increase,p < .001). The optimal antibacterial activity against E. coli was achieved with equal ratios (1:1:1) of herbal rhizomes. Metabolite analysis identified bioactive compounds including chlorogenic acid (2.3-fold increase), curcumin (4.1-fold increase), and gingerol (3.2-fold increase). Notably, fermentation reduced particle sizes to nanoscale (100–300 nm vs 1000–2000 nm in controls), enhancing bioactive compound bioavailability through increase surface area effects. The findings suggest that this fermented product has significant potential as a nutraceutical for intestinal microbiota modulation and immune system enhancement.https://www.tandfonline.com/doi/10.1080/19476337.2025.2542528Fermentationmetagenomicuntargeted metabolomicsbiological nanoparticles
spellingShingle Sofi Nabila
Arie Srihardyastutie
Aulanni’am
Tri Ardiyati
The role of bioactive compounds in coconut water-herb fermentation: formation of biological nanoparticles and their application in the modulation of intestinal microbiota
CyTA - Journal of Food
Fermentation
metagenomic
untargeted metabolomics
biological nanoparticles
title The role of bioactive compounds in coconut water-herb fermentation: formation of biological nanoparticles and their application in the modulation of intestinal microbiota
title_full The role of bioactive compounds in coconut water-herb fermentation: formation of biological nanoparticles and their application in the modulation of intestinal microbiota
title_fullStr The role of bioactive compounds in coconut water-herb fermentation: formation of biological nanoparticles and their application in the modulation of intestinal microbiota
title_full_unstemmed The role of bioactive compounds in coconut water-herb fermentation: formation of biological nanoparticles and their application in the modulation of intestinal microbiota
title_short The role of bioactive compounds in coconut water-herb fermentation: formation of biological nanoparticles and their application in the modulation of intestinal microbiota
title_sort role of bioactive compounds in coconut water herb fermentation formation of biological nanoparticles and their application in the modulation of intestinal microbiota
topic Fermentation
metagenomic
untargeted metabolomics
biological nanoparticles
url https://www.tandfonline.com/doi/10.1080/19476337.2025.2542528
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