The role of mediator subunit MED7 in Arabidopsis development
MED7, a middle-module subunit of the transcriptional co-regulator Mediator complex, plays a critical role in gene regulation in Arabidopsis thaliana, where it is encoded by two paralogs, MED7A and MED7B. We present phenotypic analyses of homozygous MED7-silenced transgenic lines with significantly r...
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1542950/full |
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| author | Koppolu Raja Rajesh Kumar Koppolu Raja Rajesh Kumar Jeanette Blomberg Stefan Björklund |
| author_facet | Koppolu Raja Rajesh Kumar Koppolu Raja Rajesh Kumar Jeanette Blomberg Stefan Björklund |
| author_sort | Koppolu Raja Rajesh Kumar |
| collection | DOAJ |
| description | MED7, a middle-module subunit of the transcriptional co-regulator Mediator complex, plays a critical role in gene regulation in Arabidopsis thaliana, where it is encoded by two paralogs, MED7A and MED7B. We present phenotypic analyses of homozygous MED7-silenced transgenic lines with significantly reduced expression of both MED7 paralogs under autotrophic conditions. Our findings demonstrate that MED7 is essential for proper cotyledon opening during de-etiolation, as the silenced lines showed a marked delay in this process. Additionally, these lines displayed distinct morphological alterations, including hyponastic cotyledons, elongated hypocotyls, and modified root architecture, such as shorter primary roots and impaired root hair development in light-grown seedlings. MED7 silencing also significantly hindered light-induced adventitious root (AR) formation on the hypocotyls of etiolated seedlings, leading to a notable reduction in AR production. Moreover, MED7 silencing impacted the timing of floral transition and shoot branching, resulting in delayed flowering and an increased number of primary cauline branches on the inflorescence stem. Together, these results underscore a central role for MED7 in orchestrating key developmental processes in plants. |
| format | Article |
| id | doaj-art-fd9051805b7041aaa50e2cd9d4cd2f08 |
| institution | DOAJ |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-fd9051805b7041aaa50e2cd9d4cd2f082025-08-20T03:02:11ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-03-011610.3389/fpls.2025.15429501542950The role of mediator subunit MED7 in Arabidopsis developmentKoppolu Raja Rajesh Kumar0Koppolu Raja Rajesh Kumar1Jeanette Blomberg2Stefan Björklund3Department of Biotechnology, Indira Gandhi National Tribal University, Amarkantak, IndiaDepartment of Medical Biochemistry and Biophysics, Umeå University, Umeå, SwedenDepartment of Medical Biochemistry and Biophysics, Umeå University, Umeå, SwedenDepartment of Medical Biochemistry and Biophysics, Umeå University, Umeå, SwedenMED7, a middle-module subunit of the transcriptional co-regulator Mediator complex, plays a critical role in gene regulation in Arabidopsis thaliana, where it is encoded by two paralogs, MED7A and MED7B. We present phenotypic analyses of homozygous MED7-silenced transgenic lines with significantly reduced expression of both MED7 paralogs under autotrophic conditions. Our findings demonstrate that MED7 is essential for proper cotyledon opening during de-etiolation, as the silenced lines showed a marked delay in this process. Additionally, these lines displayed distinct morphological alterations, including hyponastic cotyledons, elongated hypocotyls, and modified root architecture, such as shorter primary roots and impaired root hair development in light-grown seedlings. MED7 silencing also significantly hindered light-induced adventitious root (AR) formation on the hypocotyls of etiolated seedlings, leading to a notable reduction in AR production. Moreover, MED7 silencing impacted the timing of floral transition and shoot branching, resulting in delayed flowering and an increased number of primary cauline branches on the inflorescence stem. Together, these results underscore a central role for MED7 in orchestrating key developmental processes in plants.https://www.frontiersin.org/articles/10.3389/fpls.2025.1542950/fullmediatorArabidopsis thalianaMED7hyponastic cotyledonselongated hypocotylsmodified root architecture |
| spellingShingle | Koppolu Raja Rajesh Kumar Koppolu Raja Rajesh Kumar Jeanette Blomberg Stefan Björklund The role of mediator subunit MED7 in Arabidopsis development Frontiers in Plant Science mediator Arabidopsis thaliana MED7 hyponastic cotyledons elongated hypocotyls modified root architecture |
| title | The role of mediator subunit MED7 in Arabidopsis development |
| title_full | The role of mediator subunit MED7 in Arabidopsis development |
| title_fullStr | The role of mediator subunit MED7 in Arabidopsis development |
| title_full_unstemmed | The role of mediator subunit MED7 in Arabidopsis development |
| title_short | The role of mediator subunit MED7 in Arabidopsis development |
| title_sort | role of mediator subunit med7 in arabidopsis development |
| topic | mediator Arabidopsis thaliana MED7 hyponastic cotyledons elongated hypocotyls modified root architecture |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1542950/full |
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