Evaluation of CYP1B1, oxidative stress and phase II detoxification enzyme status in oral cancer progression model
Background: Tobacco is one of the main etiological factors for oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMD). CYP1B1 is an enzyme which plays a major role in the phase I detoxification of tobacco, the byproducts of which are subsequently detoxified by phase II e...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426824000241 |
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| author | Anirudha R. Bartake Sachin Sarode Sangeeta Palaskar Amit Girme Gargi Sarode Samruddhi Kamble Bindiya Narang Pradnya Bhale |
| author_facet | Anirudha R. Bartake Sachin Sarode Sangeeta Palaskar Amit Girme Gargi Sarode Samruddhi Kamble Bindiya Narang Pradnya Bhale |
| author_sort | Anirudha R. Bartake |
| collection | DOAJ |
| description | Background: Tobacco is one of the main etiological factors for oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMD). CYP1B1 is an enzyme which plays a major role in the phase I detoxification of tobacco, the byproducts of which are subsequently detoxified by phase II enzymes Glutathione S Transferase (GST). We attempted to evaluate the L432V polymorphism and tissue expression of CYP1B1, along with the oxidant-antioxidant status in OSCC progression model. Methodology: Tissue biopsies and blood samples were collected from the subjects; L432V polymorphism was evaluated by TaqMan RT-PCR, immunohistochemistry was performed on the tissue sample using CYP1B1 polyclonal primary antibody and Allred quick scoring system was used to evaluate the stained slides. Malonaldehyde (MDA) and GST activity were measured spectrophotometrically to assess oxidative-antioxidative status. Results: When the L432V polymorphism was analyzed, it was observed that in oral epithelial dysplasia (OED) and OSCC, CG was more common than GG genotype. Highest mean Allred score was observed in tobacco users (6.27), highest GST activity was seen in oral epithelial dysplasia (5.006 U/ml) and highest MDA activity was observed in OSCC (1553.94 nm/ml). Conclusion: Tobacco users with CG and GG genotypes are at equal risk of developing oral epithelial dysplasia or OSCC and L432V polymorphism does not appear to increase the risk of malignant transformation in oral epithelial dysplasia. Moreover, tobacco users with GG genotype and tissue expression of CYP1B1 may be at a greater risk of oxidative damage. |
| format | Article |
| id | doaj-art-fd8ff4534d6f404da9b341c542704174 |
| institution | OA Journals |
| issn | 2212-4268 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-fd8ff4534d6f404da9b341c5427041742025-08-20T02:02:57ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682024-03-0114216917410.1016/j.jobcr.2024.02.001Evaluation of CYP1B1, oxidative stress and phase II detoxification enzyme status in oral cancer progression modelAnirudha R. Bartake0Sachin Sarode1Sangeeta Palaskar2Amit Girme3Gargi Sarode4Samruddhi Kamble5Bindiya Narang6Pradnya Bhale7Department of Oral Pathology and Microbiology, Sinhgad Dental College and Hospital, Pune, Maharashtra, India; Department of Oral Pathology and Microbiology, Dr. D.Y. Patil Dental College & Hospital, Dr. D.Y. Patil Vidyapeeth, Pune, Maharashtra, IndiaDepartment of Oral Pathology and Microbiology, Dr. D.Y. Patil Dental College & Hospital, Dr. D.Y. Patil Vidyapeeth, Pune, Maharashtra, India; Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Dr. D.Y. Patil Unitech Society, Pimpri Pune, 411018, India; Corresponding author.Department of Oral Pathology and Microbiology, Dr. D.Y. Patil Dental College and Hospital, Dr. D.Y. Patil Vidyapeeth Sant-Tukaram Nagar, Pimpri, Pune: 18, Maharashtra, India.Department of Oral Pathology and Microbiology, Sinhgad Dental College and Hospital, Pune, Maharashtra, IndiaDepartment of Surgery, Dr. DY Patil Medical College & Research Centre, Dr. D.Y. Patil Vidyapeeth, Pune, Maharashtra, IndiaDepartment of Oral Pathology and Microbiology, Sinhgad Dental College and Hospital, Pune, Maharashtra, IndiaDepartment of Oral Pathology and Microbiology, Sinhgad Dental College and Hospital, Pune, Maharashtra, IndiaDepartment of Oral Pathology and Microbiology, Sinhgad Dental College and Hospital, Pune, Maharashtra, IndiaDepartment of Oral Pathology and Microbiology, Sinhgad Dental College and Hospital, Pune, Maharashtra, IndiaBackground: Tobacco is one of the main etiological factors for oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMD). CYP1B1 is an enzyme which plays a major role in the phase I detoxification of tobacco, the byproducts of which are subsequently detoxified by phase II enzymes Glutathione S Transferase (GST). We attempted to evaluate the L432V polymorphism and tissue expression of CYP1B1, along with the oxidant-antioxidant status in OSCC progression model. Methodology: Tissue biopsies and blood samples were collected from the subjects; L432V polymorphism was evaluated by TaqMan RT-PCR, immunohistochemistry was performed on the tissue sample using CYP1B1 polyclonal primary antibody and Allred quick scoring system was used to evaluate the stained slides. Malonaldehyde (MDA) and GST activity were measured spectrophotometrically to assess oxidative-antioxidative status. Results: When the L432V polymorphism was analyzed, it was observed that in oral epithelial dysplasia (OED) and OSCC, CG was more common than GG genotype. Highest mean Allred score was observed in tobacco users (6.27), highest GST activity was seen in oral epithelial dysplasia (5.006 U/ml) and highest MDA activity was observed in OSCC (1553.94 nm/ml). Conclusion: Tobacco users with CG and GG genotypes are at equal risk of developing oral epithelial dysplasia or OSCC and L432V polymorphism does not appear to increase the risk of malignant transformation in oral epithelial dysplasia. Moreover, tobacco users with GG genotype and tissue expression of CYP1B1 may be at a greater risk of oxidative damage.http://www.sciencedirect.com/science/article/pii/S2212426824000241CYP1B1MalonaldehydeOral squamous cell carcinomaOral epithelial dysplasiaTobacco |
| spellingShingle | Anirudha R. Bartake Sachin Sarode Sangeeta Palaskar Amit Girme Gargi Sarode Samruddhi Kamble Bindiya Narang Pradnya Bhale Evaluation of CYP1B1, oxidative stress and phase II detoxification enzyme status in oral cancer progression model Journal of Oral Biology and Craniofacial Research CYP1B1 Malonaldehyde Oral squamous cell carcinoma Oral epithelial dysplasia Tobacco |
| title | Evaluation of CYP1B1, oxidative stress and phase II detoxification enzyme status in oral cancer progression model |
| title_full | Evaluation of CYP1B1, oxidative stress and phase II detoxification enzyme status in oral cancer progression model |
| title_fullStr | Evaluation of CYP1B1, oxidative stress and phase II detoxification enzyme status in oral cancer progression model |
| title_full_unstemmed | Evaluation of CYP1B1, oxidative stress and phase II detoxification enzyme status in oral cancer progression model |
| title_short | Evaluation of CYP1B1, oxidative stress and phase II detoxification enzyme status in oral cancer progression model |
| title_sort | evaluation of cyp1b1 oxidative stress and phase ii detoxification enzyme status in oral cancer progression model |
| topic | CYP1B1 Malonaldehyde Oral squamous cell carcinoma Oral epithelial dysplasia Tobacco |
| url | http://www.sciencedirect.com/science/article/pii/S2212426824000241 |
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