MiR-129-5p influences the progression of gastric cancer cells through interacting with
The purpose of our study is to clarify the effect of microRNA-129-5p in the progression of human gastric cancer cells by regulating SPOCK1 . The expression of microRNA-129-5p and SPOCK1 was tested by quantitative real-time polymerase chain reaction in tissues and cell lines. We validated the targete...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-06-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317706916 |
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| author | Lei Yan Kai Sun Yang Liu Jun Liang Kerui Cai Jinqiu Gui |
| author_facet | Lei Yan Kai Sun Yang Liu Jun Liang Kerui Cai Jinqiu Gui |
| author_sort | Lei Yan |
| collection | DOAJ |
| description | The purpose of our study is to clarify the effect of microRNA-129-5p in the progression of human gastric cancer cells by regulating SPOCK1 . The expression of microRNA-129-5p and SPOCK1 was tested by quantitative real-time polymerase chain reaction in tissues and cell lines. We validated the targeted relationship between microRNA-129-5p and SPOCK1 by dual luciferase reporter gene assay. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, colony formation, flow cytometry, transwell, and wound scratch assays were used to analyze the effects of microRNA-129-5p on SGC-7901 cell viability, proliferation, cell cycle and apoptosis, invasiveness, and migration. MicroRNA-129-5p was downregulated while SPOCK1 was upregulated in gastric cancer tissues and cell lines. The result of luciferase reporter gene assay demonstrated that microRNA-129-5p can target SPOCK1 by binding to the 3′untranslated region. The overexpression of microRNA-129-5p or the inhibition of SPOCK1 inhibited SGC-7901 viability, proliferation, migration, and invasion while promoted cell cycle arrest in G0/G1 stage and cell apoptosis. Our results suggested that microRNA-129-5p could directly specifically suppress SPOCK1 , which might be one of the potential mechanisms in inhibiting cell processes including viability, proliferation, cell mitosis, migration, and invasiveness of gastric cancer cells. |
| format | Article |
| id | doaj-art-fd7485ebbbe94484bc8fa97af7b552a0 |
| institution | DOAJ |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-06-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-fd7485ebbbe94484bc8fa97af7b552a02025-08-20T02:49:49ZengSAGE PublishingTumor Biology1423-03802017-06-013910.1177/1010428317706916MiR-129-5p influences the progression of gastric cancer cells through interacting withLei Yan0Kai Sun1Yang Liu2Jun Liang3Kerui Cai4Jinqiu Gui5Department of Histology and Embryology, Mudanjiang Medical University, Mudanjiang, People’s Republic of ChinaDepartment of Biology, Mudanjiang Medical University, Mudanjiang, People’s Republic of ChinaDepartment of Pathogenic Microbiology and Immunology, Mudanjiang Medical University, Mudanjiang, People’s Republic of ChinaDepartment of Histology and Embryology, Mudanjiang Medical University, Mudanjiang, People’s Republic of ChinaDepartment of Histology and Embryology, Mudanjiang Medical University, Mudanjiang, People’s Republic of ChinaDepartment of Pathogenic Microbiology and Immunology, Mudanjiang Medical University, Mudanjiang, People’s Republic of ChinaThe purpose of our study is to clarify the effect of microRNA-129-5p in the progression of human gastric cancer cells by regulating SPOCK1 . The expression of microRNA-129-5p and SPOCK1 was tested by quantitative real-time polymerase chain reaction in tissues and cell lines. We validated the targeted relationship between microRNA-129-5p and SPOCK1 by dual luciferase reporter gene assay. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, colony formation, flow cytometry, transwell, and wound scratch assays were used to analyze the effects of microRNA-129-5p on SGC-7901 cell viability, proliferation, cell cycle and apoptosis, invasiveness, and migration. MicroRNA-129-5p was downregulated while SPOCK1 was upregulated in gastric cancer tissues and cell lines. The result of luciferase reporter gene assay demonstrated that microRNA-129-5p can target SPOCK1 by binding to the 3′untranslated region. The overexpression of microRNA-129-5p or the inhibition of SPOCK1 inhibited SGC-7901 viability, proliferation, migration, and invasion while promoted cell cycle arrest in G0/G1 stage and cell apoptosis. Our results suggested that microRNA-129-5p could directly specifically suppress SPOCK1 , which might be one of the potential mechanisms in inhibiting cell processes including viability, proliferation, cell mitosis, migration, and invasiveness of gastric cancer cells.https://doi.org/10.1177/1010428317706916 |
| spellingShingle | Lei Yan Kai Sun Yang Liu Jun Liang Kerui Cai Jinqiu Gui MiR-129-5p influences the progression of gastric cancer cells through interacting with Tumor Biology |
| title | MiR-129-5p influences the progression of gastric cancer cells through interacting with |
| title_full | MiR-129-5p influences the progression of gastric cancer cells through interacting with |
| title_fullStr | MiR-129-5p influences the progression of gastric cancer cells through interacting with |
| title_full_unstemmed | MiR-129-5p influences the progression of gastric cancer cells through interacting with |
| title_short | MiR-129-5p influences the progression of gastric cancer cells through interacting with |
| title_sort | mir 129 5p influences the progression of gastric cancer cells through interacting with |
| url | https://doi.org/10.1177/1010428317706916 |
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