Development of approaches to enzyme-free isolation of pancreatic islets
The success of pancreatic islet allotransplantation in the treatment of patients with a difficult-to-manage type 1 diabetes depends mainly on the quantity and quality of islets isolated from the pancreas of deceased donors using enzyme preparations, primarily collagenase. Numerous studies on improve...
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| Format: | Article |
| Language: | Russian |
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Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov
2022-04-01
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| Series: | Вестник трансплантологии и искусственных органов |
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| Online Access: | https://journal.transpl.ru/vtio/article/view/1441 |
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| author | G. N. Skaletskaya N. N. Skaletskiy G. N. Bubentsova V. I. Sevastianov |
| author_facet | G. N. Skaletskaya N. N. Skaletskiy G. N. Bubentsova V. I. Sevastianov |
| author_sort | G. N. Skaletskaya |
| collection | DOAJ |
| description | The success of pancreatic islet allotransplantation in the treatment of patients with a difficult-to-manage type 1 diabetes depends mainly on the quantity and quality of islets isolated from the pancreas of deceased donors using enzyme preparations, primarily collagenase. Numerous studies on improvement and standardization of islet isolation techniques have reached their limits in the last decade. This has made it impossible to further boost the number and quality of clinical transplants. Taking into account the negative impact of collagenase technique on the morphofunctional properties of isolated islets, this work has studied the possibility of enzyme-free isolation of islet tissue purified of exocrine ballast. Experiments using the pancreas of newborn and young rabbits showed that developing methodological approaches to obtaining islet-like cultures without the use of exogenous enzymes is feasible. |
| format | Article |
| id | doaj-art-fd62125edcfa4209bc3f91e1bceb5bda |
| institution | Kabale University |
| issn | 1995-1191 |
| language | Russian |
| publishDate | 2022-04-01 |
| publisher | Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov |
| record_format | Article |
| series | Вестник трансплантологии и искусственных органов |
| spelling | doaj-art-fd62125edcfa4209bc3f91e1bceb5bda2025-08-20T03:59:53ZrusFederal Research Center of Transplantology and Artificial Organs named after V.I.ShumakovВестник трансплантологии и искусственных органов1995-11912022-04-01241485510.15825/1995-1191-2022-1-48-551076Development of approaches to enzyme-free isolation of pancreatic isletsG. N. Skaletskaya0N. N. Skaletskiy1G. N. Bubentsova2V. I. Sevastianov3Shumakov National Medical Research Center of Transplantology and Artificial OrgansShumakov National Medical Research Center of Transplantology and Artificial OrgansShumakov National Medical Research Center of Transplantology and Artificial OrgansShumakov National Medical Research Center of Transplantology and Artificial OrgansThe success of pancreatic islet allotransplantation in the treatment of patients with a difficult-to-manage type 1 diabetes depends mainly on the quantity and quality of islets isolated from the pancreas of deceased donors using enzyme preparations, primarily collagenase. Numerous studies on improvement and standardization of islet isolation techniques have reached their limits in the last decade. This has made it impossible to further boost the number and quality of clinical transplants. Taking into account the negative impact of collagenase technique on the morphofunctional properties of isolated islets, this work has studied the possibility of enzyme-free isolation of islet tissue purified of exocrine ballast. Experiments using the pancreas of newborn and young rabbits showed that developing methodological approaches to obtaining islet-like cultures without the use of exogenous enzymes is feasible.https://journal.transpl.ru/vtio/article/view/1441pancreasexocrine tissueisletsisolationcollagenaserabbitsfloating islet-like cultures |
| spellingShingle | G. N. Skaletskaya N. N. Skaletskiy G. N. Bubentsova V. I. Sevastianov Development of approaches to enzyme-free isolation of pancreatic islets Вестник трансплантологии и искусственных органов pancreas exocrine tissue islets isolation collagenase rabbits floating islet-like cultures |
| title | Development of approaches to enzyme-free isolation of pancreatic islets |
| title_full | Development of approaches to enzyme-free isolation of pancreatic islets |
| title_fullStr | Development of approaches to enzyme-free isolation of pancreatic islets |
| title_full_unstemmed | Development of approaches to enzyme-free isolation of pancreatic islets |
| title_short | Development of approaches to enzyme-free isolation of pancreatic islets |
| title_sort | development of approaches to enzyme free isolation of pancreatic islets |
| topic | pancreas exocrine tissue islets isolation collagenase rabbits floating islet-like cultures |
| url | https://journal.transpl.ru/vtio/article/view/1441 |
| work_keys_str_mv | AT gnskaletskaya developmentofapproachestoenzymefreeisolationofpancreaticislets AT nnskaletskiy developmentofapproachestoenzymefreeisolationofpancreaticislets AT gnbubentsova developmentofapproachestoenzymefreeisolationofpancreaticislets AT visevastianov developmentofapproachestoenzymefreeisolationofpancreaticislets |