Interleukin (IL)-4 Induces Leukocyte Infiltration In Vivo by an Indirect Mechanism
Interleukin (IL)-4 is a cytokine known mainly for its anti-inflammatory activity. Using the in vivo murine air pouch model, we found that IL-4 significantly increased the number of leukocytes after 9 hours of treatment, consisting mainly of neutrophil (60%) and monocytic (40%) cell populations. Usin...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2009/193970 |
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| author | Claude Ratthé Jamila Ennaciri David M. Garcês Gonçalves Sonia Chiasson Denis Girard |
| author_facet | Claude Ratthé Jamila Ennaciri David M. Garcês Gonçalves Sonia Chiasson Denis Girard |
| author_sort | Claude Ratthé |
| collection | DOAJ |
| description | Interleukin (IL)-4 is a cytokine known mainly for its anti-inflammatory activity. Using the in vivo murine air pouch model, we found that IL-4 significantly increased the number of leukocytes after 9 hours of treatment, consisting mainly of neutrophil (60%) and monocytic (40%) cell populations. Using an antibody array, we found that the expression of several analytes (predominantly CCL2) was increased by IL-4 before the arrival of leukocytes. The IL-4-induced expression of CCL-2 was confirmed by ELISA. Air pouch resident lining cells were harvested and were found to express IL-4Rα. CCL2 mRNA expression was monitored in lining cells, cells isolated from the air pouch skin, in RAW264.7 macrophage and in epithelial Mode-K cells and its expression was increased in response to IL-4 in all conditions. We conclude that IL-4 can attract leukocytes in vivo by an indirect mechanism involving the production of several analytes by, at least, resident cells. |
| format | Article |
| id | doaj-art-fd5f70ff0e24491a984890e5e17a5333 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-fd5f70ff0e24491a984890e5e17a53332025-08-20T03:25:26ZengWileyMediators of Inflammation0962-93511466-18612009-01-01200910.1155/2009/193970193970Interleukin (IL)-4 Induces Leukocyte Infiltration In Vivo by an Indirect MechanismClaude Ratthé0Jamila Ennaciri1David M. Garcês Gonçalves2Sonia Chiasson3Denis Girard4INRS-Institut Armand-Frappier, Université du Québec, Laval, Québec, H7V 1B7, CanadaINRS-Institut Armand-Frappier, Université du Québec, Laval, Québec, H7V 1B7, CanadaINRS-Institut Armand-Frappier, Université du Québec, Laval, Québec, H7V 1B7, CanadaINRS-Institut Armand-Frappier, Université du Québec, Laval, Québec, H7V 1B7, CanadaINRS-Institut Armand-Frappier, Université du Québec, Laval, Québec, H7V 1B7, CanadaInterleukin (IL)-4 is a cytokine known mainly for its anti-inflammatory activity. Using the in vivo murine air pouch model, we found that IL-4 significantly increased the number of leukocytes after 9 hours of treatment, consisting mainly of neutrophil (60%) and monocytic (40%) cell populations. Using an antibody array, we found that the expression of several analytes (predominantly CCL2) was increased by IL-4 before the arrival of leukocytes. The IL-4-induced expression of CCL-2 was confirmed by ELISA. Air pouch resident lining cells were harvested and were found to express IL-4Rα. CCL2 mRNA expression was monitored in lining cells, cells isolated from the air pouch skin, in RAW264.7 macrophage and in epithelial Mode-K cells and its expression was increased in response to IL-4 in all conditions. We conclude that IL-4 can attract leukocytes in vivo by an indirect mechanism involving the production of several analytes by, at least, resident cells.http://dx.doi.org/10.1155/2009/193970 |
| spellingShingle | Claude Ratthé Jamila Ennaciri David M. Garcês Gonçalves Sonia Chiasson Denis Girard Interleukin (IL)-4 Induces Leukocyte Infiltration In Vivo by an Indirect Mechanism Mediators of Inflammation |
| title | Interleukin (IL)-4 Induces Leukocyte Infiltration In Vivo by an Indirect Mechanism |
| title_full | Interleukin (IL)-4 Induces Leukocyte Infiltration In Vivo by an Indirect Mechanism |
| title_fullStr | Interleukin (IL)-4 Induces Leukocyte Infiltration In Vivo by an Indirect Mechanism |
| title_full_unstemmed | Interleukin (IL)-4 Induces Leukocyte Infiltration In Vivo by an Indirect Mechanism |
| title_short | Interleukin (IL)-4 Induces Leukocyte Infiltration In Vivo by an Indirect Mechanism |
| title_sort | interleukin il 4 induces leukocyte infiltration in vivo by an indirect mechanism |
| url | http://dx.doi.org/10.1155/2009/193970 |
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