Stress exacerbates DNCB-induced atopic dermatitis in BALB/c mice: association with modulation of the gut microbiome
Atopic dermatitis (AD) is a widely recognized chronic inflammatory skin disease influenced by dietary habits, stress, genetic factors, and environmental factors. This study aimed to explore the impact of stress on AD exacerbation, as well as the associated changes in the gut microbiota. We utilized...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Stress |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10253890.2025.2525801 |
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| author | So Min Lee Keun Hyang Eom Jeeyoun Jung Jun-Chul Kang Jae-Sang Ryu You Mee Ahn Ji-Yeun Park |
| author_facet | So Min Lee Keun Hyang Eom Jeeyoun Jung Jun-Chul Kang Jae-Sang Ryu You Mee Ahn Ji-Yeun Park |
| author_sort | So Min Lee |
| collection | DOAJ |
| description | Atopic dermatitis (AD) is a widely recognized chronic inflammatory skin disease influenced by dietary habits, stress, genetic factors, and environmental factors. This study aimed to explore the impact of stress on AD exacerbation, as well as the associated changes in the gut microbiota. We utilized a 2,4-dinitrochlorobenzene (DNCB)-induced AD mouse model subjected to chronic restraint stress (CRS). The animals were divided into four groups: normal, sham control (sham), AD, and AD+CRS. Scratching behavior was significantly increased in the AD+CRS group compared to the AD group on day 28, indicating that stress exacerbates pruritus in AD. Relative abundance analysis of the gut microbiota at the phylum level revealed an increased relative abundance of Bacteroidota in both the AD and AD+CRS groups. Principal coordinate analysis revealed distinct patterns between the AD and AD+CRS groups. The relative abundance of Heminiphilus was negatively correlated with immunoglobulin E (IgE) levels, while the relative abundance of Ruminococcus exhibited significant and negative correlations with both corticosterone and IgE levels. Alistipes, which is known to aggravate AD, was notably elevated in the AD+CRS group. These findings confirm that stress-related changes in the gut microbiota composition may contribute to the exacerbation of AD, highlighting the connection among stress, immune response, and microbiome dynamics in AD progression. |
| format | Article |
| id | doaj-art-fd56f71f8e0741369eb86aba97f52868 |
| institution | Kabale University |
| issn | 1025-3890 1607-8888 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Stress |
| spelling | doaj-art-fd56f71f8e0741369eb86aba97f528682025-08-20T03:28:52ZengTaylor & Francis GroupStress1025-38901607-88882025-12-0128110.1080/10253890.2025.2525801Stress exacerbates DNCB-induced atopic dermatitis in BALB/c mice: association with modulation of the gut microbiomeSo Min Lee0Keun Hyang Eom1Jeeyoun Jung2Jun-Chul Kang3Jae-Sang Ryu4You Mee Ahn5Ji-Yeun Park6KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon, Republic of KoreaCollege of Korean Medicine, Daejeon University, Daejeon, Republic of KoreaKM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon, Republic of KoreaCollege of Korean Medicine, Daejeon University, Daejeon, Republic of KoreaCollege of Korean Medicine, Daejeon University, Daejeon, Republic of KoreaKM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon, Republic of KoreaCollege of Korean Medicine, Daejeon University, Daejeon, Republic of KoreaAtopic dermatitis (AD) is a widely recognized chronic inflammatory skin disease influenced by dietary habits, stress, genetic factors, and environmental factors. This study aimed to explore the impact of stress on AD exacerbation, as well as the associated changes in the gut microbiota. We utilized a 2,4-dinitrochlorobenzene (DNCB)-induced AD mouse model subjected to chronic restraint stress (CRS). The animals were divided into four groups: normal, sham control (sham), AD, and AD+CRS. Scratching behavior was significantly increased in the AD+CRS group compared to the AD group on day 28, indicating that stress exacerbates pruritus in AD. Relative abundance analysis of the gut microbiota at the phylum level revealed an increased relative abundance of Bacteroidota in both the AD and AD+CRS groups. Principal coordinate analysis revealed distinct patterns between the AD and AD+CRS groups. The relative abundance of Heminiphilus was negatively correlated with immunoglobulin E (IgE) levels, while the relative abundance of Ruminococcus exhibited significant and negative correlations with both corticosterone and IgE levels. Alistipes, which is known to aggravate AD, was notably elevated in the AD+CRS group. These findings confirm that stress-related changes in the gut microbiota composition may contribute to the exacerbation of AD, highlighting the connection among stress, immune response, and microbiome dynamics in AD progression.https://www.tandfonline.com/doi/10.1080/10253890.2025.2525801Atopic dermatitischronic restraint stressEASImast cellepidermis thicknessmicrobiome |
| spellingShingle | So Min Lee Keun Hyang Eom Jeeyoun Jung Jun-Chul Kang Jae-Sang Ryu You Mee Ahn Ji-Yeun Park Stress exacerbates DNCB-induced atopic dermatitis in BALB/c mice: association with modulation of the gut microbiome Stress Atopic dermatitis chronic restraint stress EASI mast cell epidermis thickness microbiome |
| title | Stress exacerbates DNCB-induced atopic dermatitis in BALB/c mice: association with modulation of the gut microbiome |
| title_full | Stress exacerbates DNCB-induced atopic dermatitis in BALB/c mice: association with modulation of the gut microbiome |
| title_fullStr | Stress exacerbates DNCB-induced atopic dermatitis in BALB/c mice: association with modulation of the gut microbiome |
| title_full_unstemmed | Stress exacerbates DNCB-induced atopic dermatitis in BALB/c mice: association with modulation of the gut microbiome |
| title_short | Stress exacerbates DNCB-induced atopic dermatitis in BALB/c mice: association with modulation of the gut microbiome |
| title_sort | stress exacerbates dncb induced atopic dermatitis in balb c mice association with modulation of the gut microbiome |
| topic | Atopic dermatitis chronic restraint stress EASI mast cell epidermis thickness microbiome |
| url | https://www.tandfonline.com/doi/10.1080/10253890.2025.2525801 |
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