Escherichia coli in septic arthritis: prevalence and antibiotics susceptibility patterns
Background and Objectives: Septic arthritis (SA) is an orthopedic emergency mainly caused by bacteria. SA due to Escherichia coli (E. coli) is rare with a poor prognosis. This study aimed to assess the occurrence and antibiotic resistance patterns of E. coli in SA patients in Quetta, Balochistan, P...
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| Language: | English |
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Tehran University of Medical Sciences
2025-02-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4761 |
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| author | Masroora Ali Khan Muhammad Kamran Taj Zahid Khan Imran Taj Muhammad Naeem Saima Azam |
| author_facet | Masroora Ali Khan Muhammad Kamran Taj Zahid Khan Imran Taj Muhammad Naeem Saima Azam |
| author_sort | Masroora Ali Khan |
| collection | DOAJ |
| description |
Background and Objectives: Septic arthritis (SA) is an orthopedic emergency mainly caused by bacteria. SA due to Escherichia coli (E. coli) is rare with a poor prognosis. This study aimed to assess the occurrence and antibiotic resistance patterns of E. coli in SA patients in Quetta, Balochistan, Pakistan.
Materials and Methods: A cross-sectional study was conducted from March 2021 to December 2023. 220 samples were collected from SA patients from tertiary care hospitals. Joint aspirates (2ml) and blood (5ml) were analyzed for microbial and hematological examination.
Results: There were 5.45% samples positive, and 94.5% negative for E. coli. SA due to E. coli was more common in male (6.2%) than female (4.6%) patients with the knee being the most affected joint (6.3%). E. coli was more common in patients aged 41-60 years (7.7%), lower socioeconomic (6.9%), and illiterate (8.6%) patients. Suspected patients showed a significant increase in the levels of white blood cells (WBC), C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR), notably, these levels were further elevated in E. coli-positive patients. The polymerase chain reaction (PCR) based identification of E. coli showed clear bands of 204bp of the 16S rRNA gene. Sequence analysis using the Basic Local Alignment Search Tool found high similarity with pathogenic E. coli from Egypt and China. The identified E. coli strain showed significant resistance to common antibiotics: amoxicillin, amoxicillin-clavulanate, ceftriaxone, sulfamethoxazole/trimethoprim, gentamicin, tetracycline, and erythromycin.
Conclusion: Antibiotic resistance in E. coli from SA patients suggests the need for accurate antibiotic selection to ensure prompt treatment.
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| format | Article |
| id | doaj-art-fd4e2b63bd754fc5bf8372ab683495fb |
| institution | Kabale University |
| issn | 2008-3289 2008-4447 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj-art-fd4e2b63bd754fc5bf8372ab683495fb2025-02-09T09:01:34ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472025-02-01171Escherichia coli in septic arthritis: prevalence and antibiotics susceptibility patternsMasroora Ali Khan0Muhammad Kamran Taj1Zahid Khan2Imran Taj3Muhammad Naeem4Saima Azam5Department of Microbiology, Center for Advanced Studies in Vaccinology and Biotechnology, University of Balochistan, Quetta, Pakistan; Department of Microbiology, Sardar Bahadur Khan Women’s University, Quetta, PakistanDepartment of Microbiology, Center for Advanced Studies in Vaccinology and Biotechnology, University of Balochistan, Quetta, PakistanDepartment of Orthopaedics Surgery, Sandeman Provincial Hospital, Quetta, PakistanDepartment of Microbiology, Center for Advanced Studies in Vaccinology and Biotechnology, University of Balochistan, Quetta, PakistanDepartment of Microbiology, Center for Advanced Studies in Vaccinology and Biotechnology, University of Balochistan, Quetta, PakistanDepartment of Microbiology, Center for Advanced Studies in Vaccinology and Biotechnology, University of Balochistan, Quetta, Pakistan Background and Objectives: Septic arthritis (SA) is an orthopedic emergency mainly caused by bacteria. SA due to Escherichia coli (E. coli) is rare with a poor prognosis. This study aimed to assess the occurrence and antibiotic resistance patterns of E. coli in SA patients in Quetta, Balochistan, Pakistan. Materials and Methods: A cross-sectional study was conducted from March 2021 to December 2023. 220 samples were collected from SA patients from tertiary care hospitals. Joint aspirates (2ml) and blood (5ml) were analyzed for microbial and hematological examination. Results: There were 5.45% samples positive, and 94.5% negative for E. coli. SA due to E. coli was more common in male (6.2%) than female (4.6%) patients with the knee being the most affected joint (6.3%). E. coli was more common in patients aged 41-60 years (7.7%), lower socioeconomic (6.9%), and illiterate (8.6%) patients. Suspected patients showed a significant increase in the levels of white blood cells (WBC), C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR), notably, these levels were further elevated in E. coli-positive patients. The polymerase chain reaction (PCR) based identification of E. coli showed clear bands of 204bp of the 16S rRNA gene. Sequence analysis using the Basic Local Alignment Search Tool found high similarity with pathogenic E. coli from Egypt and China. The identified E. coli strain showed significant resistance to common antibiotics: amoxicillin, amoxicillin-clavulanate, ceftriaxone, sulfamethoxazole/trimethoprim, gentamicin, tetracycline, and erythromycin. Conclusion: Antibiotic resistance in E. coli from SA patients suggests the need for accurate antibiotic selection to ensure prompt treatment. https://ijm.tums.ac.ir/index.php/ijm/article/view/4761Antibiotic resistance;Escherichia coli;Septic arthritis;Sequence analysis |
| spellingShingle | Masroora Ali Khan Muhammad Kamran Taj Zahid Khan Imran Taj Muhammad Naeem Saima Azam Escherichia coli in septic arthritis: prevalence and antibiotics susceptibility patterns Iranian Journal of Microbiology Antibiotic resistance; Escherichia coli; Septic arthritis; Sequence analysis |
| title | Escherichia coli in septic arthritis: prevalence and antibiotics susceptibility patterns |
| title_full | Escherichia coli in septic arthritis: prevalence and antibiotics susceptibility patterns |
| title_fullStr | Escherichia coli in septic arthritis: prevalence and antibiotics susceptibility patterns |
| title_full_unstemmed | Escherichia coli in septic arthritis: prevalence and antibiotics susceptibility patterns |
| title_short | Escherichia coli in septic arthritis: prevalence and antibiotics susceptibility patterns |
| title_sort | escherichia coli in septic arthritis prevalence and antibiotics susceptibility patterns |
| topic | Antibiotic resistance; Escherichia coli; Septic arthritis; Sequence analysis |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4761 |
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