Differences in the effects and action modes of gut commensals against dextran sulfate sodium-induced intestinal inflammation
Inflammatory bowel disease (IBD) is a complex relapsing inflammatory disease in the gut and is driven by complicated host-gut microbiome interactions. Gut commensals have shown different functions in IBD prevention and treatment. To gain a mechanistic understanding of how different commensals affect...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2024-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250100 |
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| author | Dingwu Qu Zhennan Gu Saisai Feng Leilei Yu Fengwei Tian Hao Zhang Wei Chen Qixiao Zhai |
| author_facet | Dingwu Qu Zhennan Gu Saisai Feng Leilei Yu Fengwei Tian Hao Zhang Wei Chen Qixiao Zhai |
| author_sort | Dingwu Qu |
| collection | DOAJ |
| description | Inflammatory bowel disease (IBD) is a complex relapsing inflammatory disease in the gut and is driven by complicated host-gut microbiome interactions. Gut commensals have shown different functions in IBD prevention and treatment. To gain a mechanistic understanding of how different commensals affect intestinal inflammation, we compared the protective effects of 6 probiotics (belonging to the genera Akkermansia, Bifidobacterium, Clostridium, and Enterococcus) on dextran sulfate sodium (DSS)-induced colitis in mice with or without gut microbiota. Anti-inflammatory properties (ratio of interleukin (IL)-10 and IL-12) of these strains were also evaluated in an in vitro mesenteric lymph nodes (MLN) co-culture system. Results showed that 4 probiotics (belonging to the species Bifidobacterium breve, Bifidobacterium bifidum, and Enterococcus faecalis) can alleviate colitis in normal mice. The probiotic strains differed in regulating the intestinal microbiota, cytokines (IL-10, IL-1β and interferon (IFN)-γ), and tight junction function (Zonulin-1 and Occludin). By constrast, Akkermansia muciniphila AH39 and Clostridium butyricum FHuNHHMY49T1 were not protective. Interestingly, B. breve JSNJJNM2 with high anti-inflammatory potential in the MLN model could relieve colitis symptoms in antibiotic cocktail (Abx)-treated mice. Meanwhile, E. faecalis FJSWX25M1 induced low levels of cytokines in vitro and showed no beneficial effects. Therefore, we provided insight into the clinical application of probiotics in IBD treatment. |
| format | Article |
| id | doaj-art-fd4d928856694744b30ad36986863c43 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-fd4d928856694744b30ad36986863c432025-01-10T06:54:23ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-05-011331201121110.26599/FSHW.2022.9250100Differences in the effects and action modes of gut commensals against dextran sulfate sodium-induced intestinal inflammationDingwu Qu0Zhennan Gu1Saisai Feng2Leilei Yu3Fengwei Tian4Hao Zhang5Wei Chen6Qixiao Zhai7State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaInflammatory bowel disease (IBD) is a complex relapsing inflammatory disease in the gut and is driven by complicated host-gut microbiome interactions. Gut commensals have shown different functions in IBD prevention and treatment. To gain a mechanistic understanding of how different commensals affect intestinal inflammation, we compared the protective effects of 6 probiotics (belonging to the genera Akkermansia, Bifidobacterium, Clostridium, and Enterococcus) on dextran sulfate sodium (DSS)-induced colitis in mice with or without gut microbiota. Anti-inflammatory properties (ratio of interleukin (IL)-10 and IL-12) of these strains were also evaluated in an in vitro mesenteric lymph nodes (MLN) co-culture system. Results showed that 4 probiotics (belonging to the species Bifidobacterium breve, Bifidobacterium bifidum, and Enterococcus faecalis) can alleviate colitis in normal mice. The probiotic strains differed in regulating the intestinal microbiota, cytokines (IL-10, IL-1β and interferon (IFN)-γ), and tight junction function (Zonulin-1 and Occludin). By constrast, Akkermansia muciniphila AH39 and Clostridium butyricum FHuNHHMY49T1 were not protective. Interestingly, B. breve JSNJJNM2 with high anti-inflammatory potential in the MLN model could relieve colitis symptoms in antibiotic cocktail (Abx)-treated mice. Meanwhile, E. faecalis FJSWX25M1 induced low levels of cytokines in vitro and showed no beneficial effects. Therefore, we provided insight into the clinical application of probiotics in IBD treatment.https://www.sciopen.com/article/10.26599/FSHW.2022.9250100gut commensalsdextran sulfate sodium (dss) colitisintestinal barrierimmunoregulation |
| spellingShingle | Dingwu Qu Zhennan Gu Saisai Feng Leilei Yu Fengwei Tian Hao Zhang Wei Chen Qixiao Zhai Differences in the effects and action modes of gut commensals against dextran sulfate sodium-induced intestinal inflammation Food Science and Human Wellness gut commensals dextran sulfate sodium (dss) colitis intestinal barrier immunoregulation |
| title | Differences in the effects and action modes of gut commensals against dextran sulfate sodium-induced intestinal inflammation |
| title_full | Differences in the effects and action modes of gut commensals against dextran sulfate sodium-induced intestinal inflammation |
| title_fullStr | Differences in the effects and action modes of gut commensals against dextran sulfate sodium-induced intestinal inflammation |
| title_full_unstemmed | Differences in the effects and action modes of gut commensals against dextran sulfate sodium-induced intestinal inflammation |
| title_short | Differences in the effects and action modes of gut commensals against dextran sulfate sodium-induced intestinal inflammation |
| title_sort | differences in the effects and action modes of gut commensals against dextran sulfate sodium induced intestinal inflammation |
| topic | gut commensals dextran sulfate sodium (dss) colitis intestinal barrier immunoregulation |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250100 |
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