Anthocyanins and flavonoids derived from Clitoria ternatea L. flower inhibit bladder cancer growth via suppressing fatty acid synthesis mediated by SREBP1 pathway
Clitoria ternatea L. flowers are used as traditional herbal medicines and are known for their advanced pharmacological activities. Flavonoids and anthocyanins reportedly contribute to the therapeutic properties of C. ternatea flowers; however, their potential...
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| Format: | Article |
| Language: | English |
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China Science Publishing & Media Ltd.
2024-10-01
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| Series: | Acta Biochimica et Biophysica Sinica |
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| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2024192 |
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| author | Liu Chenkai Liu Jue Liu Gao Song Yusong Yang Xiuyu Gao Honglei Xiang Cheng Sang Jie Xu Tianrui Sang Jun |
| author_facet | Liu Chenkai Liu Jue Liu Gao Song Yusong Yang Xiuyu Gao Honglei Xiang Cheng Sang Jie Xu Tianrui Sang Jun |
| author_sort | Liu Chenkai |
| collection | DOAJ |
| description | Clitoria ternatea L. flowers are used as traditional herbal medicines and are known for their advanced pharmacological activities. Flavonoids and anthocyanins reportedly contribute to the therapeutic properties of C. ternatea flowers; however, their potential anti-bladder cancer effects and molecular mechanisms remain unknown. In this study, flavonoid- and anthocyanin-rich samples from C. ternatea flowers (DDH) are prepared via macroporous resin-based extraction coupled with an efficient and reliable two-dimensional UPLC-DAD-MS/MS method. In vitro and in vivo studies reveal that DDH can inhibit bladder cancer cell growth and enhance the anti-bladder cancer activity of cisplatin. RNA-seq combined with KEGG analysis reveals that fatty acid synthesis is closely related to the anti-bladder cancer effect of DDH. Furthermore, DDH dose-dependently reduces cellular fatty acid levels in bladder cancer cells, and the addition of fatty acids significantly mitigates DDH-induced cell growth inhibition. Subsequent findings reveal that DDH downregulates sterol regulatory element-binding protein 1 (SREBP1), a key transcriptional regulator of de novo fatty acid synthesis in cancer cells, and its downstream targets (FASN, SCD1, and ACC). Additionally, this study demonstrates that gallic acid not only enhances the stability of DDH but also synergistically potentiates its anti-bladder cancer activity. Our study suggests that targeting the SREBP1 pathway is an effective strategy in bladder cancer therapy, and the ability of DDH to induce cell death by inhibiting the SREBP1 pathway and its good tolerance in mice make it a promising strategy for preventing and treating bladder cancer. |
| format | Article |
| id | doaj-art-fd4b7ca685f34b3e8ee8cff615118841 |
| institution | DOAJ |
| issn | 1672-9145 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj-art-fd4b7ca685f34b3e8ee8cff6151188412025-08-20T03:05:45ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452024-10-015777078110.3724/abbs.202419220d259ccAnthocyanins and flavonoids derived from Clitoria ternatea L. flower inhibit bladder cancer growth via suppressing fatty acid synthesis mediated by SREBP1 pathwayLiu Chenkai0Liu Jue1Liu Gao2Song Yusong3Yang Xiuyu4Gao Honglei5Xiang Cheng6Sang Jie7Xu Tianrui8Sang Jun9["Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China"]["Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China"]["Yunnan University of Traditional Chinese Medicine, Kunming 650500, China"]["Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China"]["Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China"]["Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China"]["Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China"]["School of Medicine, Ankang University, Ankang 725000, China"]["Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China"]["Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China"] Clitoria ternatea L. flowers are used as traditional herbal medicines and are known for their advanced pharmacological activities. Flavonoids and anthocyanins reportedly contribute to the therapeutic properties of C. ternatea flowers; however, their potential anti-bladder cancer effects and molecular mechanisms remain unknown. In this study, flavonoid- and anthocyanin-rich samples from C. ternatea flowers (DDH) are prepared via macroporous resin-based extraction coupled with an efficient and reliable two-dimensional UPLC-DAD-MS/MS method. In vitro and in vivo studies reveal that DDH can inhibit bladder cancer cell growth and enhance the anti-bladder cancer activity of cisplatin. RNA-seq combined with KEGG analysis reveals that fatty acid synthesis is closely related to the anti-bladder cancer effect of DDH. Furthermore, DDH dose-dependently reduces cellular fatty acid levels in bladder cancer cells, and the addition of fatty acids significantly mitigates DDH-induced cell growth inhibition. Subsequent findings reveal that DDH downregulates sterol regulatory element-binding protein 1 (SREBP1), a key transcriptional regulator of de novo fatty acid synthesis in cancer cells, and its downstream targets (FASN, SCD1, and ACC). Additionally, this study demonstrates that gallic acid not only enhances the stability of DDH but also synergistically potentiates its anti-bladder cancer activity. Our study suggests that targeting the SREBP1 pathway is an effective strategy in bladder cancer therapy, and the ability of DDH to induce cell death by inhibiting the SREBP1 pathway and its good tolerance in mice make it a promising strategy for preventing and treating bladder cancer.https://www.sciengine.com/doi/10.3724/abbs.2024192combination therapybladder cancerextraction optimizationlipogenesisphytochemicalstwo-dimensional UPLC-DAD-MS/MS |
| spellingShingle | Liu Chenkai Liu Jue Liu Gao Song Yusong Yang Xiuyu Gao Honglei Xiang Cheng Sang Jie Xu Tianrui Sang Jun Anthocyanins and flavonoids derived from Clitoria ternatea L. flower inhibit bladder cancer growth via suppressing fatty acid synthesis mediated by SREBP1 pathway Acta Biochimica et Biophysica Sinica combination therapy bladder cancer extraction optimization lipogenesis phytochemicals two-dimensional UPLC-DAD-MS/MS |
| title | Anthocyanins and flavonoids derived from Clitoria ternatea L. flower inhibit bladder cancer growth via suppressing fatty acid synthesis mediated by SREBP1 pathway |
| title_full | Anthocyanins and flavonoids derived from Clitoria ternatea L. flower inhibit bladder cancer growth via suppressing fatty acid synthesis mediated by SREBP1 pathway |
| title_fullStr | Anthocyanins and flavonoids derived from Clitoria ternatea L. flower inhibit bladder cancer growth via suppressing fatty acid synthesis mediated by SREBP1 pathway |
| title_full_unstemmed | Anthocyanins and flavonoids derived from Clitoria ternatea L. flower inhibit bladder cancer growth via suppressing fatty acid synthesis mediated by SREBP1 pathway |
| title_short | Anthocyanins and flavonoids derived from Clitoria ternatea L. flower inhibit bladder cancer growth via suppressing fatty acid synthesis mediated by SREBP1 pathway |
| title_sort | anthocyanins and flavonoids derived from clitoria ternatea l flower inhibit bladder cancer growth via suppressing fatty acid synthesis mediated by srebp1 pathway |
| topic | combination therapy bladder cancer extraction optimization lipogenesis phytochemicals two-dimensional UPLC-DAD-MS/MS |
| url | https://www.sciengine.com/doi/10.3724/abbs.2024192 |
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