Study on the mechanism of no. 8 burn ointment in burn treatment based on network pharmacology and experimental verification

IntroductionAs a traditional Chinese compound formulation, No.8 burn ointment has demonstrated notable efficacy over half a century of clinical use, particularly in facilitating the healing of burn wounds.MethodsThis study integrates clinical data, liquid chromatography-mass spectrometry, network ph...

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Main Authors: Le Qiu, Danlei Xing, Yuanqiang Zhu, Zhiwei Zhuang, Yexiang Sun, Peng Gong, Xulin Chen
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-07-01
Series:Frontiers in Pharmacology
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Online Access:https://www.frontiersin.org/articles/10.3389/fphar.2025.1511741/full
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author Le Qiu
Danlei Xing
Yuanqiang Zhu
Zhiwei Zhuang
Yexiang Sun
Peng Gong
Xulin Chen
author_facet Le Qiu
Danlei Xing
Yuanqiang Zhu
Zhiwei Zhuang
Yexiang Sun
Peng Gong
Xulin Chen
author_sort Le Qiu
collection DOAJ
description IntroductionAs a traditional Chinese compound formulation, No.8 burn ointment has demonstrated notable efficacy over half a century of clinical use, particularly in facilitating the healing of burn wounds.MethodsThis study integrates clinical data, liquid chromatography-mass spectrometry, network pharmacology and experimental validation to elucidate the mechanisms by which No.8 burn ointment promotes burn wound healing and to identify its key active components.ResultsThe findings reveal that the ointment may accelerate wound repair by modulating the HIF-1 signaling pathway. The network pharmacology analysis has identified HIF-1α as a core target of the No.8 burn ointment’s action and has identified the key active molecules β-Elemonic acid and Rubiadin, which interact with the HIF-1α protein and influence the HIF-1 pathway.ConclusionThis research not only provides a theoretical foundation for the selection of targeted drugs in burn treatment and the optimization of traditional Chinese compounds but also provides new ideas for future research in related fields.
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publishDate 2025-07-01
publisher Frontiers Media S.A.
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spelling doaj-art-fd49b67989714f2caf5589fc918e3f012025-08-20T03:51:19ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-07-011610.3389/fphar.2025.15117411511741Study on the mechanism of no. 8 burn ointment in burn treatment based on network pharmacology and experimental verificationLe Qiu0Danlei Xing1Yuanqiang Zhu2Zhiwei Zhuang3Yexiang Sun4Peng Gong5Xulin Chen6Department of Burns, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Burns, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Burns, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Burns, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Burns, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Pharmacy, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Burns, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaIntroductionAs a traditional Chinese compound formulation, No.8 burn ointment has demonstrated notable efficacy over half a century of clinical use, particularly in facilitating the healing of burn wounds.MethodsThis study integrates clinical data, liquid chromatography-mass spectrometry, network pharmacology and experimental validation to elucidate the mechanisms by which No.8 burn ointment promotes burn wound healing and to identify its key active components.ResultsThe findings reveal that the ointment may accelerate wound repair by modulating the HIF-1 signaling pathway. The network pharmacology analysis has identified HIF-1α as a core target of the No.8 burn ointment’s action and has identified the key active molecules β-Elemonic acid and Rubiadin, which interact with the HIF-1α protein and influence the HIF-1 pathway.ConclusionThis research not only provides a theoretical foundation for the selection of targeted drugs in burn treatment and the optimization of traditional Chinese compounds but also provides new ideas for future research in related fields.https://www.frontiersin.org/articles/10.3389/fphar.2025.1511741/fullNo.8 burn ointmentnetwork pharmacologyburnHIF-1αmolecular docking
spellingShingle Le Qiu
Danlei Xing
Yuanqiang Zhu
Zhiwei Zhuang
Yexiang Sun
Peng Gong
Xulin Chen
Study on the mechanism of no. 8 burn ointment in burn treatment based on network pharmacology and experimental verification
Frontiers in Pharmacology
No.8 burn ointment
network pharmacology
burn
HIF-1α
molecular docking
title Study on the mechanism of no. 8 burn ointment in burn treatment based on network pharmacology and experimental verification
title_full Study on the mechanism of no. 8 burn ointment in burn treatment based on network pharmacology and experimental verification
title_fullStr Study on the mechanism of no. 8 burn ointment in burn treatment based on network pharmacology and experimental verification
title_full_unstemmed Study on the mechanism of no. 8 burn ointment in burn treatment based on network pharmacology and experimental verification
title_short Study on the mechanism of no. 8 burn ointment in burn treatment based on network pharmacology and experimental verification
title_sort study on the mechanism of no 8 burn ointment in burn treatment based on network pharmacology and experimental verification
topic No.8 burn ointment
network pharmacology
burn
HIF-1α
molecular docking
url https://www.frontiersin.org/articles/10.3389/fphar.2025.1511741/full
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