New approach methodologies for risk assessment using deep learning

Abstract The advancement of technologies and the development of more efficient artificial intelligence (AI) enable the processing of large amounts of data in a very short time. Concurrently, the increase in information within biological databases, such as 3D molecular structures or networks of funct...

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Main Authors: Enol Junquera, Irene Díaz, Susana Montes, Ferdinando Febbraio
Format: Article
Language:English
Published: Wiley 2024-12-01
Series:EFSA Journal
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Online Access:https://doi.org/10.2903/j.efsa.2024.e221105
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author Enol Junquera
Irene Díaz
Susana Montes
Ferdinando Febbraio
author_facet Enol Junquera
Irene Díaz
Susana Montes
Ferdinando Febbraio
author_sort Enol Junquera
collection DOAJ
description Abstract The advancement of technologies and the development of more efficient artificial intelligence (AI) enable the processing of large amounts of data in a very short time. Concurrently, the increase in information within biological databases, such as 3D molecular structures or networks of functional macromolecule associations, will facilitate the creation of new methods for risk assessment that can serve as alternatives to animal testing. Specifically, the predictive capabilities of AI as new approach methodologies (NAMs) are poised to revolutionise risk assessment approaches. Our previous studies on molecular docking predictions, using the software Autodock Vina, indicated high‐affinity binding of certain toxic chemicals to the 3D structures of human proteins associated with nervous and reproductive functions. Similar approaches revealed potential sublethal interactions of neonicotinoids with proteins linked to the bees' immune system. Building on these findings, we plan to develop an AI‐based decision tool that exploits the data available on the toxicity of the most know chemical, such as LD50, and the data obtainable by their interaction with the human proteins to support risk assessment studies for multiple stressors still not characterised. Our focus will be on utilising these new bioinformatics methodologies to develop specific experimental designs that allow for confident and predictable study of the toxic and sublethal effects of pesticides on humans. We will also validate the developed NAMs by integrating existing in vivo information from scientific literature and technical reports. These approaches will significantly impact toxicity studies, guiding researchers' experiments and greatly reducing the need for animal testing.
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spelling doaj-art-fd2f8ca32f934f1990e686aa2f2c7d8c2025-08-20T02:35:30ZengWileyEFSA Journal1831-47322024-12-0122S1n/an/a10.2903/j.efsa.2024.e221105New approach methodologies for risk assessment using deep learningEnol Junquera0Irene Díaz1Susana Montes2Ferdinando Febbraio3University of Oviedo Oviedo SpainUniversity of Oviedo Oviedo SpainUniversity of Oviedo Oviedo SpainInstitute of Biochemistry and Cell Biology National Research Council (CNR) Naples ItalyAbstract The advancement of technologies and the development of more efficient artificial intelligence (AI) enable the processing of large amounts of data in a very short time. Concurrently, the increase in information within biological databases, such as 3D molecular structures or networks of functional macromolecule associations, will facilitate the creation of new methods for risk assessment that can serve as alternatives to animal testing. Specifically, the predictive capabilities of AI as new approach methodologies (NAMs) are poised to revolutionise risk assessment approaches. Our previous studies on molecular docking predictions, using the software Autodock Vina, indicated high‐affinity binding of certain toxic chemicals to the 3D structures of human proteins associated with nervous and reproductive functions. Similar approaches revealed potential sublethal interactions of neonicotinoids with proteins linked to the bees' immune system. Building on these findings, we plan to develop an AI‐based decision tool that exploits the data available on the toxicity of the most know chemical, such as LD50, and the data obtainable by their interaction with the human proteins to support risk assessment studies for multiple stressors still not characterised. Our focus will be on utilising these new bioinformatics methodologies to develop specific experimental designs that allow for confident and predictable study of the toxic and sublethal effects of pesticides on humans. We will also validate the developed NAMs by integrating existing in vivo information from scientific literature and technical reports. These approaches will significantly impact toxicity studies, guiding researchers' experiments and greatly reducing the need for animal testing.https://doi.org/10.2903/j.efsa.2024.e221105artificial intelligencemolecular dockingmolecular stressorspesticide toxicityprotein 3D structurerisk assessment
spellingShingle Enol Junquera
Irene Díaz
Susana Montes
Ferdinando Febbraio
New approach methodologies for risk assessment using deep learning
EFSA Journal
artificial intelligence
molecular docking
molecular stressors
pesticide toxicity
protein 3D structure
risk assessment
title New approach methodologies for risk assessment using deep learning
title_full New approach methodologies for risk assessment using deep learning
title_fullStr New approach methodologies for risk assessment using deep learning
title_full_unstemmed New approach methodologies for risk assessment using deep learning
title_short New approach methodologies for risk assessment using deep learning
title_sort new approach methodologies for risk assessment using deep learning
topic artificial intelligence
molecular docking
molecular stressors
pesticide toxicity
protein 3D structure
risk assessment
url https://doi.org/10.2903/j.efsa.2024.e221105
work_keys_str_mv AT enoljunquera newapproachmethodologiesforriskassessmentusingdeeplearning
AT irenediaz newapproachmethodologiesforriskassessmentusingdeeplearning
AT susanamontes newapproachmethodologiesforriskassessmentusingdeeplearning
AT ferdinandofebbraio newapproachmethodologiesforriskassessmentusingdeeplearning