Deterministic and stochastic allele specific gene expression in single mouse blastomeres.
Stochastic and deterministic allele specific gene expression (ASE) might influence single cell phenotype, but the extent and nature of the phenomenon at the onset of early mouse development is unknown. Here we performed single cell RNA-Seq analysis of single blastomeres of mouse embryos, which revea...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0021208&type=printable |
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| author | Fuchou Tang Catalin Barbacioru Ellen Nordman Siqin Bao Caroline Lee Xiaohui Wang Brian B Tuch Edith Heard Kaiqin Lao M Azim Surani |
| author_facet | Fuchou Tang Catalin Barbacioru Ellen Nordman Siqin Bao Caroline Lee Xiaohui Wang Brian B Tuch Edith Heard Kaiqin Lao M Azim Surani |
| author_sort | Fuchou Tang |
| collection | DOAJ |
| description | Stochastic and deterministic allele specific gene expression (ASE) might influence single cell phenotype, but the extent and nature of the phenomenon at the onset of early mouse development is unknown. Here we performed single cell RNA-Seq analysis of single blastomeres of mouse embryos, which revealed significant changes in the transcriptome. Importantly, over half of the transcripts with detectable genetic polymorphisms exhibit ASE, most notably, individual blastomeres from the same two-cell embryo show similar pattern of ASE. However, about 6% of them exhibit stochastic expression, indicated by altered expression ratio between the two alleles. Thus, we demonstrate that ASE is both deterministic and stochastic in early blastomeres. Furthermore, we also found that 1,718 genes express two isoforms with different lengths of 3'UTRs, with the shorter one on average 5-6 times more abundant in early blastomeres compared to the transcripts in epiblast cells, suggesting that microRNA mediated regulation of gene expression acquires increasing importance as development progresses. |
| format | Article |
| id | doaj-art-fd2ac4e7b75244b8bc77b5063a64f55a |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-fd2ac4e7b75244b8bc77b5063a64f55a2025-08-20T02:30:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0166e2120810.1371/journal.pone.0021208Deterministic and stochastic allele specific gene expression in single mouse blastomeres.Fuchou TangCatalin BarbacioruEllen NordmanSiqin BaoCaroline LeeXiaohui WangBrian B TuchEdith HeardKaiqin LaoM Azim SuraniStochastic and deterministic allele specific gene expression (ASE) might influence single cell phenotype, but the extent and nature of the phenomenon at the onset of early mouse development is unknown. Here we performed single cell RNA-Seq analysis of single blastomeres of mouse embryos, which revealed significant changes in the transcriptome. Importantly, over half of the transcripts with detectable genetic polymorphisms exhibit ASE, most notably, individual blastomeres from the same two-cell embryo show similar pattern of ASE. However, about 6% of them exhibit stochastic expression, indicated by altered expression ratio between the two alleles. Thus, we demonstrate that ASE is both deterministic and stochastic in early blastomeres. Furthermore, we also found that 1,718 genes express two isoforms with different lengths of 3'UTRs, with the shorter one on average 5-6 times more abundant in early blastomeres compared to the transcripts in epiblast cells, suggesting that microRNA mediated regulation of gene expression acquires increasing importance as development progresses.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0021208&type=printable |
| spellingShingle | Fuchou Tang Catalin Barbacioru Ellen Nordman Siqin Bao Caroline Lee Xiaohui Wang Brian B Tuch Edith Heard Kaiqin Lao M Azim Surani Deterministic and stochastic allele specific gene expression in single mouse blastomeres. PLoS ONE |
| title | Deterministic and stochastic allele specific gene expression in single mouse blastomeres. |
| title_full | Deterministic and stochastic allele specific gene expression in single mouse blastomeres. |
| title_fullStr | Deterministic and stochastic allele specific gene expression in single mouse blastomeres. |
| title_full_unstemmed | Deterministic and stochastic allele specific gene expression in single mouse blastomeres. |
| title_short | Deterministic and stochastic allele specific gene expression in single mouse blastomeres. |
| title_sort | deterministic and stochastic allele specific gene expression in single mouse blastomeres |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0021208&type=printable |
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