Optimized Extraction of Resveratrol from Arachis repens Handro by Ultrasound and Microwave: A Correlation Study with the Antioxidant Properties and Phenol Contents
The vegetal species Arachis repens, commonly known as peanut grass, was studied and, for the first time, we detected the presence of the bioactive compound trans-resveratrol (t-RSV). We compared the efficiency of three different methodologies (conventional maceration [CM], ultrasound-assisted extrac...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2016/5890897 |
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| author | Leonardo Garcia Renata Garcia Georgia Pacheco Felipe Sutili Rodrigo De Souza Elisabeth Mansur Ivana Leal |
| author_facet | Leonardo Garcia Renata Garcia Georgia Pacheco Felipe Sutili Rodrigo De Souza Elisabeth Mansur Ivana Leal |
| author_sort | Leonardo Garcia |
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| description | The vegetal species Arachis repens, commonly known as peanut grass, was studied and, for the first time, we detected the presence of the bioactive compound trans-resveratrol (t-RSV). We compared the efficiency of three different methodologies (conventional maceration [CM], ultrasound-assisted extractions [UAE], and microwave-assisted extractions [MAE]) concerning total phenolics (TP) and resveratrol (t-RSV) content, followed by antioxidant activity (AA) evaluation. By CM, at 1 h, the highest RSV content (1.024±0.036 mg/L) and, correspondingly, the highest DPPH capture (23.90±0.04%) were found. The TP contents, at 1 h, presented the highest value (27.26±0.26 mg/g GAE). By the UAE, the maximum yields of TP (357.18 mg/g GAE) and RSV (2.14 mg/L), as well as, the highest AA (70.95%), were obtained by 5 min after a maceration pretreatment, on the solid-solvent ratio 1 : 40 w/v. For MAE, a central composite rotatable design (CCRD) was applied followed by the FFD design in order to evaluate the statistical effects of four independent variables on the extraction of RSV. The optimal conditions established for obtaining the highest recovery (2.516 mg/g) were 20 min; 90% MeOH aq.; 120 rpm; 60°C; and solid-solvent ratio: 1 : 35 w/v. Relevant correlations were established considering the TP and RSV contents, as well as the AA, corroborating obvious advantages of such techniques in terms of high extraction efficiency in shorter times. |
| format | Article |
| id | doaj-art-fd23e175d0ba4f579ed2301274993c0b |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-fd23e175d0ba4f579ed2301274993c0b2025-02-03T01:31:46ZengWileyThe Scientific World Journal2356-61401537-744X2016-01-01201610.1155/2016/58908975890897Optimized Extraction of Resveratrol from Arachis repens Handro by Ultrasound and Microwave: A Correlation Study with the Antioxidant Properties and Phenol ContentsLeonardo Garcia0Renata Garcia1Georgia Pacheco2Felipe Sutili3Rodrigo De Souza4Elisabeth Mansur5Ivana Leal6Laboratório de Micropropagação e Transformação de Plantas, Cellular Biology Department, State University of Rio de Janeiro, Rio de Janeiro, RJ, BrazilLaboratório de Micropropagação e Transformação de Plantas, Cellular Biology Department, State University of Rio de Janeiro, Rio de Janeiro, RJ, BrazilLaboratório de Micropropagação e Transformação de Plantas, Cellular Biology Department, State University of Rio de Janeiro, Rio de Janeiro, RJ, BrazilDepartamento de Engenharia de Bioprocessos e Biotecnologia, Universidade Estadual Paulista (UNESP), Campus Botucatu, 18610 307 Botucatu, SP, BrazilBiocatalysis and Organic Synthesis Group, Chemistry Institute, Federal University of Rio de Janeiro, 22941-909 Rio de Janeiro, RJ, BrazilLaboratório de Micropropagação e Transformação de Plantas, Cellular Biology Department, State University of Rio de Janeiro, Rio de Janeiro, RJ, BrazilLaboratório de Produtos Naturais e Ensaios Biológicos (LaProNEB), Pharmacy Faculty, Department of Natural Products and Food, Federal University of Rio de Janeiro, 21.941-902 Rio de Janeiro, RJ, BrazilThe vegetal species Arachis repens, commonly known as peanut grass, was studied and, for the first time, we detected the presence of the bioactive compound trans-resveratrol (t-RSV). We compared the efficiency of three different methodologies (conventional maceration [CM], ultrasound-assisted extractions [UAE], and microwave-assisted extractions [MAE]) concerning total phenolics (TP) and resveratrol (t-RSV) content, followed by antioxidant activity (AA) evaluation. By CM, at 1 h, the highest RSV content (1.024±0.036 mg/L) and, correspondingly, the highest DPPH capture (23.90±0.04%) were found. The TP contents, at 1 h, presented the highest value (27.26±0.26 mg/g GAE). By the UAE, the maximum yields of TP (357.18 mg/g GAE) and RSV (2.14 mg/L), as well as, the highest AA (70.95%), were obtained by 5 min after a maceration pretreatment, on the solid-solvent ratio 1 : 40 w/v. For MAE, a central composite rotatable design (CCRD) was applied followed by the FFD design in order to evaluate the statistical effects of four independent variables on the extraction of RSV. The optimal conditions established for obtaining the highest recovery (2.516 mg/g) were 20 min; 90% MeOH aq.; 120 rpm; 60°C; and solid-solvent ratio: 1 : 35 w/v. Relevant correlations were established considering the TP and RSV contents, as well as the AA, corroborating obvious advantages of such techniques in terms of high extraction efficiency in shorter times.http://dx.doi.org/10.1155/2016/5890897 |
| spellingShingle | Leonardo Garcia Renata Garcia Georgia Pacheco Felipe Sutili Rodrigo De Souza Elisabeth Mansur Ivana Leal Optimized Extraction of Resveratrol from Arachis repens Handro by Ultrasound and Microwave: A Correlation Study with the Antioxidant Properties and Phenol Contents The Scientific World Journal |
| title | Optimized Extraction of Resveratrol from Arachis repens Handro by Ultrasound and Microwave: A Correlation Study with the Antioxidant Properties and Phenol Contents |
| title_full | Optimized Extraction of Resveratrol from Arachis repens Handro by Ultrasound and Microwave: A Correlation Study with the Antioxidant Properties and Phenol Contents |
| title_fullStr | Optimized Extraction of Resveratrol from Arachis repens Handro by Ultrasound and Microwave: A Correlation Study with the Antioxidant Properties and Phenol Contents |
| title_full_unstemmed | Optimized Extraction of Resveratrol from Arachis repens Handro by Ultrasound and Microwave: A Correlation Study with the Antioxidant Properties and Phenol Contents |
| title_short | Optimized Extraction of Resveratrol from Arachis repens Handro by Ultrasound and Microwave: A Correlation Study with the Antioxidant Properties and Phenol Contents |
| title_sort | optimized extraction of resveratrol from arachis repens handro by ultrasound and microwave a correlation study with the antioxidant properties and phenol contents |
| url | http://dx.doi.org/10.1155/2016/5890897 |
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