Evaluation of Antioxidant Capacity of Solanum sessiliflorum (Cubiu) Extract: An In Vitro Assay
Cubiu is a vegetable of Solanaceae family, native to the Amazon, which is widely distributed through Brazil, Peru, and Colombia. It is used in food, medicine, and cosmetics by native populations. Research has shown that cubiu extracts have antioxidant activities with great biological relevance. We p...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Nutrition and Metabolism |
| Online Access: | http://dx.doi.org/10.1155/2015/364185 |
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| author | Diego Rocha de Lucena Herrera Mascato Janice B. Monteiro Michele M. Passarinho Denise Morais Lopes Galeno Rubén J. Cruz Carmen Ortiz Luisa Morales Emerson Silva Lima Rosany Piccolotto Carvalho |
| author_facet | Diego Rocha de Lucena Herrera Mascato Janice B. Monteiro Michele M. Passarinho Denise Morais Lopes Galeno Rubén J. Cruz Carmen Ortiz Luisa Morales Emerson Silva Lima Rosany Piccolotto Carvalho |
| author_sort | Diego Rocha de Lucena Herrera Mascato |
| collection | DOAJ |
| description | Cubiu is a vegetable of Solanaceae family, native to the Amazon, which is widely distributed through Brazil, Peru, and Colombia. It is used in food, medicine, and cosmetics by native populations. Research has shown that cubiu extracts have antioxidant activities with great biological relevance. We performed a phytochemical screening to identify the main chemical groups that could confer antioxidant activity to this extract. Several tests and qualitative precipitation specific staining for major classes of secondary metabolites were used. Antioxidant capacity in vitro tests (DPPH and ABTS) were also used to assess the extract’s ability to sequester free radicals of 70% hydroethanolic and aqueous extracts of cubiu flour. Alkaloids, organic acids, phenols, flavonoid glycosides, and coumarins were found in the hydroethanolic extract while the aqueous extract presented anthocyanins, gums, tannins and mucilage, amino groups, and volatile and fixed acids. For in vitro tests, the IC50 value obtained in the DPPH assay was 606.3 ± 3.5 μg/mL while that for the ABTS assay was 290.3 ± 10.7 µg/mL. Although cubiu extracts present chemical compounds directly related to antioxidant activity, our results show that it has a low antioxidant activity. Additional studies will be needed to isolate and characterize specific compounds to further assess antioxidant activity. |
| format | Article |
| id | doaj-art-fd22e0dd9da44adcbd7186688bccb682 |
| institution | Kabale University |
| issn | 2090-0724 2090-0732 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nutrition and Metabolism |
| spelling | doaj-art-fd22e0dd9da44adcbd7186688bccb6822025-08-20T03:25:26ZengWileyJournal of Nutrition and Metabolism2090-07242090-07322015-01-01201510.1155/2015/364185364185Evaluation of Antioxidant Capacity of Solanum sessiliflorum (Cubiu) Extract: An In Vitro AssayDiego Rocha de Lucena Herrera Mascato0Janice B. Monteiro1Michele M. Passarinho2Denise Morais Lopes Galeno3Rubén J. Cruz4Carmen Ortiz5Luisa Morales6Emerson Silva Lima7Rosany Piccolotto Carvalho8Programa Multi-Institucional de Pós-Graduação em Biotecnologia, Universidade Federal do Amazonas, 69077-000 Manaus, AM, BrazilDivision of Biochemistry, Ponce Health Sciences University, School of Medicine, Ponce Research Institute, Ponce, PR 00732-7004, USAPrograma Multi-Institucional de Pós-Graduação em Biotecnologia, Universidade Federal do Amazonas, 69077-000 Manaus, AM, BrazilPrograma Multi-Institucional de Pós-Graduação em Biotecnologia, Universidade Federal do Amazonas, 69077-000 Manaus, AM, BrazilBiology Department, University of Puerto Rico at Ponce, Ponce, PR 00734, USADivisions of Pharmacology & Toxicology & Cancer Biology, Ponce Health Sciences University, School of Medicine, Ponce Research Institute, Ponce, PR 00732-7004, USAPublic Health Program, Ponce Health Sciences University, Ponce, PR 00732-7004, USAFaculdade de Ciências Farmacêuticas, Universidade Federal do Amazonas, 69010-300 Manaus, AM, BrazilPrograma Multi-Institucional de Pós-Graduação em Biotecnologia, Universidade Federal do Amazonas, 69077-000 Manaus, AM, BrazilCubiu is a vegetable of Solanaceae family, native to the Amazon, which is widely distributed through Brazil, Peru, and Colombia. It is used in food, medicine, and cosmetics by native populations. Research has shown that cubiu extracts have antioxidant activities with great biological relevance. We performed a phytochemical screening to identify the main chemical groups that could confer antioxidant activity to this extract. Several tests and qualitative precipitation specific staining for major classes of secondary metabolites were used. Antioxidant capacity in vitro tests (DPPH and ABTS) were also used to assess the extract’s ability to sequester free radicals of 70% hydroethanolic and aqueous extracts of cubiu flour. Alkaloids, organic acids, phenols, flavonoid glycosides, and coumarins were found in the hydroethanolic extract while the aqueous extract presented anthocyanins, gums, tannins and mucilage, amino groups, and volatile and fixed acids. For in vitro tests, the IC50 value obtained in the DPPH assay was 606.3 ± 3.5 μg/mL while that for the ABTS assay was 290.3 ± 10.7 µg/mL. Although cubiu extracts present chemical compounds directly related to antioxidant activity, our results show that it has a low antioxidant activity. Additional studies will be needed to isolate and characterize specific compounds to further assess antioxidant activity.http://dx.doi.org/10.1155/2015/364185 |
| spellingShingle | Diego Rocha de Lucena Herrera Mascato Janice B. Monteiro Michele M. Passarinho Denise Morais Lopes Galeno Rubén J. Cruz Carmen Ortiz Luisa Morales Emerson Silva Lima Rosany Piccolotto Carvalho Evaluation of Antioxidant Capacity of Solanum sessiliflorum (Cubiu) Extract: An In Vitro Assay Journal of Nutrition and Metabolism |
| title | Evaluation of Antioxidant Capacity of Solanum sessiliflorum (Cubiu) Extract: An In Vitro Assay |
| title_full | Evaluation of Antioxidant Capacity of Solanum sessiliflorum (Cubiu) Extract: An In Vitro Assay |
| title_fullStr | Evaluation of Antioxidant Capacity of Solanum sessiliflorum (Cubiu) Extract: An In Vitro Assay |
| title_full_unstemmed | Evaluation of Antioxidant Capacity of Solanum sessiliflorum (Cubiu) Extract: An In Vitro Assay |
| title_short | Evaluation of Antioxidant Capacity of Solanum sessiliflorum (Cubiu) Extract: An In Vitro Assay |
| title_sort | evaluation of antioxidant capacity of solanum sessiliflorum cubiu extract an in vitro assay |
| url | http://dx.doi.org/10.1155/2015/364185 |
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