Automated Indirect Immunofluorescence Evaluation of Antinuclear Autoantibodies on HEp-2 Cells
Indirect immunofluorescence (IIF) on human epithelial (HEp-2) cells is considered as the gold standard screening method for the detection of antinuclear autoantibodies (ANA). However, in terms of automation and standardization, it has not been able to keep pace with most other analytical techniques...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/651058 |
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| author | Jörn Voigt Christopher Krause Edda Rohwäder Sandra Saschenbrecker Melanie Hahn Maick Danckwardt Christian Feirer Konstantin Ens Kai Fechner Erhardt Barth Thomas Martinetz Winfried Stöcker |
| author_facet | Jörn Voigt Christopher Krause Edda Rohwäder Sandra Saschenbrecker Melanie Hahn Maick Danckwardt Christian Feirer Konstantin Ens Kai Fechner Erhardt Barth Thomas Martinetz Winfried Stöcker |
| author_sort | Jörn Voigt |
| collection | DOAJ |
| description | Indirect immunofluorescence (IIF) on human epithelial (HEp-2) cells is considered as the gold standard screening method for the detection of antinuclear autoantibodies (ANA). However, in terms of automation and standardization, it has not been able to keep pace with most other analytical techniques used in diagnostic laboratories. Although there are already some automation solutions for IIF incubation in the market, the automation of result evaluation is still in its infancy. Therefore, the EUROPattern Suite has been developed as a comprehensive automated processing and interpretation system for standardized and efficient ANA detection by HEp-2 cell-based IIF. In this study, the automated pattern recognition was compared to conventional visual interpretation in a total of 351 sera. In the discrimination of positive from negative samples, concordant results between visual and automated evaluation were obtained for 349 sera (99.4%, kappa = 0.984). The system missed out none of the 272 antibody-positive samples and identified 77 out of 79 visually negative samples (analytical sensitivity/specificity: 100%/97.5%). Moreover, 94.0% of all main antibody patterns were recognized correctly by the software. Owing to its performance characteristics, EUROPattern enables fast, objective, and economic IIF ANA analysis and has the potential to reduce intra- and interlaboratory variability. |
| format | Article |
| id | doaj-art-fd15ef22e6e24fb2969dcec5e11d35a1 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
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| series | Clinical and Developmental Immunology |
| spelling | doaj-art-fd15ef22e6e24fb2969dcec5e11d35a12025-02-03T05:54:01ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/651058651058Automated Indirect Immunofluorescence Evaluation of Antinuclear Autoantibodies on HEp-2 CellsJörn Voigt0Christopher Krause1Edda Rohwäder2Sandra Saschenbrecker3Melanie Hahn4Maick Danckwardt5Christian Feirer6Konstantin Ens7Kai Fechner8Erhardt Barth9Thomas Martinetz10Winfried Stöcker11Institute for Experimental Immunology, Euroimmun AG, Seekamp 31, 23560 Lübeck, GermanyInstitute for Experimental Immunology, Euroimmun AG, Seekamp 31, 23560 Lübeck, GermanyInstitute for Experimental Immunology, Euroimmun AG, Seekamp 31, 23560 Lübeck, GermanyInstitute for Experimental Immunology, Euroimmun AG, Seekamp 31, 23560 Lübeck, GermanyInstitute for Experimental Immunology, Euroimmun AG, Seekamp 31, 23560 Lübeck, GermanyInstitute for Experimental Immunology, Euroimmun AG, Seekamp 31, 23560 Lübeck, GermanyInstitute for Experimental Immunology, Euroimmun AG, Seekamp 31, 23560 Lübeck, GermanyInstitute for Experimental Immunology, Euroimmun AG, Seekamp 31, 23560 Lübeck, GermanyInstitute for Experimental Immunology, Euroimmun AG, Seekamp 31, 23560 Lübeck, GermanyInstitute for Neuro- and Bioinformatics, University of Lübeck, Ratzeburger Allee 160, 23562 Lübeck, GermanyInstitute for Neuro- and Bioinformatics, University of Lübeck, Ratzeburger Allee 160, 23562 Lübeck, GermanyInstitute for Experimental Immunology, Euroimmun AG, Seekamp 31, 23560 Lübeck, GermanyIndirect immunofluorescence (IIF) on human epithelial (HEp-2) cells is considered as the gold standard screening method for the detection of antinuclear autoantibodies (ANA). However, in terms of automation and standardization, it has not been able to keep pace with most other analytical techniques used in diagnostic laboratories. Although there are already some automation solutions for IIF incubation in the market, the automation of result evaluation is still in its infancy. Therefore, the EUROPattern Suite has been developed as a comprehensive automated processing and interpretation system for standardized and efficient ANA detection by HEp-2 cell-based IIF. In this study, the automated pattern recognition was compared to conventional visual interpretation in a total of 351 sera. In the discrimination of positive from negative samples, concordant results between visual and automated evaluation were obtained for 349 sera (99.4%, kappa = 0.984). The system missed out none of the 272 antibody-positive samples and identified 77 out of 79 visually negative samples (analytical sensitivity/specificity: 100%/97.5%). Moreover, 94.0% of all main antibody patterns were recognized correctly by the software. Owing to its performance characteristics, EUROPattern enables fast, objective, and economic IIF ANA analysis and has the potential to reduce intra- and interlaboratory variability.http://dx.doi.org/10.1155/2012/651058 |
| spellingShingle | Jörn Voigt Christopher Krause Edda Rohwäder Sandra Saschenbrecker Melanie Hahn Maick Danckwardt Christian Feirer Konstantin Ens Kai Fechner Erhardt Barth Thomas Martinetz Winfried Stöcker Automated Indirect Immunofluorescence Evaluation of Antinuclear Autoantibodies on HEp-2 Cells Clinical and Developmental Immunology |
| title | Automated Indirect Immunofluorescence Evaluation of Antinuclear Autoantibodies on HEp-2 Cells |
| title_full | Automated Indirect Immunofluorescence Evaluation of Antinuclear Autoantibodies on HEp-2 Cells |
| title_fullStr | Automated Indirect Immunofluorescence Evaluation of Antinuclear Autoantibodies on HEp-2 Cells |
| title_full_unstemmed | Automated Indirect Immunofluorescence Evaluation of Antinuclear Autoantibodies on HEp-2 Cells |
| title_short | Automated Indirect Immunofluorescence Evaluation of Antinuclear Autoantibodies on HEp-2 Cells |
| title_sort | automated indirect immunofluorescence evaluation of antinuclear autoantibodies on hep 2 cells |
| url | http://dx.doi.org/10.1155/2012/651058 |
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