Bioassay of conidial thermotolerance of Beauveria bassiana strains from different hosts and geographic origins
In order to search for fungal biocontrol agents adaptable to pest control during hot summer of southern China, aerial conidia of 12 Beauveria bassiana strains from different host insects and geographic origins were separately suspended in a solution containing 2% sucrose and 0.5% peptone. For each s...
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Zhejiang University Press
2008-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.02.006 |
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| author | LI Hong-wen FENG Ming-guang |
| author_facet | LI Hong-wen FENG Ming-guang |
| author_sort | LI Hong-wen |
| collection | DOAJ |
| description | In order to search for fungal biocontrol agents adaptable to pest control during hot summer of southern China, aerial conidia of 12 Beauveria bassiana strains from different host insects and geographic origins were separately suspended in a solution containing 2% sucrose and 0.5% peptone. For each strain, the conidial suspension of 1 mL in a 10 mL glass tube was exposed to the thermal stress of 48 ℃in water bath. The residual viability of the conidia after a given period of exposure was measured as survival index, which was defined as the ratio of their germination rate (observed after 24 h incubation at 25 ℃) over the germination rate of unexposed conidia (blank control). Observations made at 6 min intervals during the thermal stress fitted well to a logistic equation (r<sup>2</sup>>0.98), which yielded the estimates of median lethal time (LT<sub>50</sub>) depicting fungal thermotolerance. As a result, the LT<sub>50</sub> estimates fell in a range of 6.7-21.2 min, being significantly different among the 12 strains tested. The method developed is simple and convenient for the evaluation of conidial thermotolerance, and could be useful for screening of thermotolerable fungal biocontrol agents and enhancement of their field persistence. |
| format | Article |
| id | doaj-art-fd0b6e1b987249f2b87ffcc0fbfa6f49 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2008-03-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-fd0b6e1b987249f2b87ffcc0fbfa6f492025-08-20T03:16:11ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552008-03-013415816210.3785/j.issn.1008-9209.2008.02.00610089209Bioassay of conidial thermotolerance of Beauveria bassiana strains from different hosts and geographic originsLI Hong-wenFENG Ming-guangIn order to search for fungal biocontrol agents adaptable to pest control during hot summer of southern China, aerial conidia of 12 Beauveria bassiana strains from different host insects and geographic origins were separately suspended in a solution containing 2% sucrose and 0.5% peptone. For each strain, the conidial suspension of 1 mL in a 10 mL glass tube was exposed to the thermal stress of 48 ℃in water bath. The residual viability of the conidia after a given period of exposure was measured as survival index, which was defined as the ratio of their germination rate (observed after 24 h incubation at 25 ℃) over the germination rate of unexposed conidia (blank control). Observations made at 6 min intervals during the thermal stress fitted well to a logistic equation (r<sup>2</sup>>0.98), which yielded the estimates of median lethal time (LT<sub>50</sub>) depicting fungal thermotolerance. As a result, the LT<sub>50</sub> estimates fell in a range of 6.7-21.2 min, being significantly different among the 12 strains tested. The method developed is simple and convenient for the evaluation of conidial thermotolerance, and could be useful for screening of thermotolerable fungal biocontrol agents and enhancement of their field persistence.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.02.006<italic>Beauveria bassiana</italic>fungal biocontrol agentsconidial thermotolerancethermal stressmedian lethal time |
| spellingShingle | LI Hong-wen FENG Ming-guang Bioassay of conidial thermotolerance of Beauveria bassiana strains from different hosts and geographic origins 浙江大学学报. 农业与生命科学版 <italic>Beauveria bassiana</italic> fungal biocontrol agents conidial thermotolerance thermal stress median lethal time |
| title | Bioassay of conidial thermotolerance of Beauveria bassiana strains from different hosts and geographic origins |
| title_full | Bioassay of conidial thermotolerance of Beauveria bassiana strains from different hosts and geographic origins |
| title_fullStr | Bioassay of conidial thermotolerance of Beauveria bassiana strains from different hosts and geographic origins |
| title_full_unstemmed | Bioassay of conidial thermotolerance of Beauveria bassiana strains from different hosts and geographic origins |
| title_short | Bioassay of conidial thermotolerance of Beauveria bassiana strains from different hosts and geographic origins |
| title_sort | bioassay of conidial thermotolerance of beauveria bassiana strains from different hosts and geographic origins |
| topic | <italic>Beauveria bassiana</italic> fungal biocontrol agents conidial thermotolerance thermal stress median lethal time |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.02.006 |
| work_keys_str_mv | AT lihongwen bioassayofconidialthermotoleranceofbeauveriabassianastrainsfromdifferenthostsandgeographicorigins AT fengmingguang bioassayofconidialthermotoleranceofbeauveriabassianastrainsfromdifferenthostsandgeographicorigins |