A Comparative Study on Application of Computer Vision and Fluorescence Imaging Spectroscopy for Detection of Huanglongbing Citrus Disease in the USA and Brazil

The overall objective of this work was to develop and evaluate computer vision and machine learning technique for classification of Huanglongbing-(HLB)-infected and healthy leaves using fluorescence imaging spectroscopy. The fluorescence images were segmented using normalized graph cut, and texture...

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Main Authors: Caio B. Wetterich, Ratnesh Kumar, Sindhuja Sankaran, José Belasque Junior, Reza Ehsani, Luis G. Marcassa
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:Journal of Spectroscopy
Online Access:http://dx.doi.org/10.1155/2013/841738
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author Caio B. Wetterich
Ratnesh Kumar
Sindhuja Sankaran
José Belasque Junior
Reza Ehsani
Luis G. Marcassa
author_facet Caio B. Wetterich
Ratnesh Kumar
Sindhuja Sankaran
José Belasque Junior
Reza Ehsani
Luis G. Marcassa
author_sort Caio B. Wetterich
collection DOAJ
description The overall objective of this work was to develop and evaluate computer vision and machine learning technique for classification of Huanglongbing-(HLB)-infected and healthy leaves using fluorescence imaging spectroscopy. The fluorescence images were segmented using normalized graph cut, and texture features were extracted from the segmented images using cooccurrence matrix. The extracted features were used as an input into the classifier, support vector machine (SVM). The classification results were evaluated based on classification accuracies and number of false positives and false negatives. The results indicated that the SVM could classify HLB-infected leaf fluorescence intensities with up to 90% classification accuracy. Though the fluorescence intensities from leaves collected in Brazil and the USA were different, the method shows potential for detecting HLB.
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issn 2314-4920
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publishDate 2013-01-01
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spelling doaj-art-fd074aea07224f228bbb3bb17e796b2f2025-02-03T00:59:44ZengWileyJournal of Spectroscopy2314-49202314-49392013-01-01201310.1155/2013/841738841738A Comparative Study on Application of Computer Vision and Fluorescence Imaging Spectroscopy for Detection of Huanglongbing Citrus Disease in the USA and BrazilCaio B. Wetterich0Ratnesh Kumar1Sindhuja Sankaran2José Belasque Junior3Reza Ehsani4Luis G. Marcassa5Instituto de Física de São Carlos, Universidade de São Paulo, Cx. Postal 369, 13560-970 São Carlos, SP, BrazilCitrus Research and Education Center, IFAS, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USACitrus Research and Education Center, IFAS, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USADepartamento Científico, Fundecitrus, Avenida Dr. Adhemar P. de Barros, 20114 807-040 Araraquara, SP, BrazilCitrus Research and Education Center, IFAS, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USAInstituto de Física de São Carlos, Universidade de São Paulo, Cx. Postal 369, 13560-970 São Carlos, SP, BrazilThe overall objective of this work was to develop and evaluate computer vision and machine learning technique for classification of Huanglongbing-(HLB)-infected and healthy leaves using fluorescence imaging spectroscopy. The fluorescence images were segmented using normalized graph cut, and texture features were extracted from the segmented images using cooccurrence matrix. The extracted features were used as an input into the classifier, support vector machine (SVM). The classification results were evaluated based on classification accuracies and number of false positives and false negatives. The results indicated that the SVM could classify HLB-infected leaf fluorescence intensities with up to 90% classification accuracy. Though the fluorescence intensities from leaves collected in Brazil and the USA were different, the method shows potential for detecting HLB.http://dx.doi.org/10.1155/2013/841738
spellingShingle Caio B. Wetterich
Ratnesh Kumar
Sindhuja Sankaran
José Belasque Junior
Reza Ehsani
Luis G. Marcassa
A Comparative Study on Application of Computer Vision and Fluorescence Imaging Spectroscopy for Detection of Huanglongbing Citrus Disease in the USA and Brazil
Journal of Spectroscopy
title A Comparative Study on Application of Computer Vision and Fluorescence Imaging Spectroscopy for Detection of Huanglongbing Citrus Disease in the USA and Brazil
title_full A Comparative Study on Application of Computer Vision and Fluorescence Imaging Spectroscopy for Detection of Huanglongbing Citrus Disease in the USA and Brazil
title_fullStr A Comparative Study on Application of Computer Vision and Fluorescence Imaging Spectroscopy for Detection of Huanglongbing Citrus Disease in the USA and Brazil
title_full_unstemmed A Comparative Study on Application of Computer Vision and Fluorescence Imaging Spectroscopy for Detection of Huanglongbing Citrus Disease in the USA and Brazil
title_short A Comparative Study on Application of Computer Vision and Fluorescence Imaging Spectroscopy for Detection of Huanglongbing Citrus Disease in the USA and Brazil
title_sort comparative study on application of computer vision and fluorescence imaging spectroscopy for detection of huanglongbing citrus disease in the usa and brazil
url http://dx.doi.org/10.1155/2013/841738
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