Isolation, screening, and molecular identification of endopytic fungus producing cellulose and cyanide degrading enzyme its application for waste cassava.
Objective: This research aims to isolate, screen, and identify some candidates for endophytic fungus-producing cellulase and cyanidase. Materials and Methods: Fungi were isolated from cassava leaves that had undergone surface sterilization. The fungal isolates were qualitatively tested for their ab...
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Network for the Veterinarians of Bangladesh
2025-03-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=220864 |
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| author | Yetti Marlida Husmaini Husmaini Ahadiyah Yuniza Lili Anggraini Wulansih Dwi Astuti Ridho Kurniawan Rusli Hera Dwi Triani Gusri Yanti. |
| author_facet | Yetti Marlida Husmaini Husmaini Ahadiyah Yuniza Lili Anggraini Wulansih Dwi Astuti Ridho Kurniawan Rusli Hera Dwi Triani Gusri Yanti. |
| author_sort | Yetti Marlida |
| collection | DOAJ |
| description | Objective: This research aims to isolate, screen, and identify some candidates for endophytic fungus-producing cellulase and cyanidase.
Materials and Methods: Fungi were isolated from cassava leaves that had undergone surface sterilization. The fungal isolates were qualitatively tested for their ability to produce cellulase and cyanidase enzymes by adding carboxy methyl cellulose (CMC) and KCN to the media. Enzyme production was indicated by the formation of clear zones around the growing colonies. Isolates that tested positive for cellulase and cyanidase production underwent further quantitative screening to measure enzyme activity using a spectrophotometer at wavelengths of 540 nm and 400 nm, respectively. The isolates showing the highest cellulase and cyanidase activity were identified through 18S rRNA analysis using the Sanger DNA sequencing method.
Results: The research obtained six pure isolates of endophytic fungus, namely Y1; Y2; Y3; Y4; Y5; and Y6. Four isolates had the ability to degrade CMC with a clear zone between 0.1 until 0.5 mm, and three isolates had the ability for KCN degrade. The highest activity for cellulase and cyanidase degrading enzymes was produced by isolate Y2. After molecular identification using 18S rRNA, isolate Y2 had 98.82% similarity to Phomopsis sp. 32PG/F.
Conclusion: Six isolates of endophytic fungi were obtained, Y1; Y2; Y3; Y4; Y5; and Y6. Four iso¬late the ability of to degrade CMC and three isolate the ability for KCN degrade. Isolate Y2 is the isolate with the best activity for cellulase and cyanidase degrading enzymes, namely 2.99 U/ml and 2.19 U/ml. After molecular identification using 18S rRNA, isolate Y2 had 98.82% similarity to Phomopsis sp. 32PG/F. [J Adv Vet Anim Res 2025; 12(1.000): 169-178] |
| format | Article |
| id | doaj-art-fd06e712d17341a9a7d6fbeb5c794bf7 |
| institution | DOAJ |
| issn | 2311-7710 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj-art-fd06e712d17341a9a7d6fbeb5c794bf72025-08-20T03:14:41ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102025-03-0112116917810.5455/javar.2025.l884220864Isolation, screening, and molecular identification of endopytic fungus producing cellulose and cyanide degrading enzyme its application for waste cassava.Yetti Marlida0Husmaini Husmaini1Ahadiyah Yuniza2Lili Anggraini3Wulansih Dwi Astuti4Ridho Kurniawan Rusli5Hera Dwi Triani6Gusri Yanti.7Department of Animal Nutrition, Faculty of Animal Science, Universitas Andalas, Padang, Indonesia Department of Animal Production, Faculty of Animal Science, Universitas Andalas, Padang, Indonesia Department of Animal Nutrition, Faculty of Animal Science, Universitas Andalas, Padang, Indonesia Research Center for Animal Husbandry, National Research and Innovation Agency (BRIN), Cibinong, Indonesia Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Cibinong, Indonesia Department of Animal Nutrition, Faculty of Animal Science, Universitas Andalas, Padang, Indonesia Department of Agricultural Extension, Faculty of Social, Science and Education, Universitas Prima Nusantara, Bukittinggi, Indonesia Department of Agricultural Extension, Faculty of Social, Science and Education, Universitas Prima Nusantara, Bukittinggi, Indonesia.Objective: This research aims to isolate, screen, and identify some candidates for endophytic fungus-producing cellulase and cyanidase. Materials and Methods: Fungi were isolated from cassava leaves that had undergone surface sterilization. The fungal isolates were qualitatively tested for their ability to produce cellulase and cyanidase enzymes by adding carboxy methyl cellulose (CMC) and KCN to the media. Enzyme production was indicated by the formation of clear zones around the growing colonies. Isolates that tested positive for cellulase and cyanidase production underwent further quantitative screening to measure enzyme activity using a spectrophotometer at wavelengths of 540 nm and 400 nm, respectively. The isolates showing the highest cellulase and cyanidase activity were identified through 18S rRNA analysis using the Sanger DNA sequencing method. Results: The research obtained six pure isolates of endophytic fungus, namely Y1; Y2; Y3; Y4; Y5; and Y6. Four isolates had the ability to degrade CMC with a clear zone between 0.1 until 0.5 mm, and three isolates had the ability for KCN degrade. The highest activity for cellulase and cyanidase degrading enzymes was produced by isolate Y2. After molecular identification using 18S rRNA, isolate Y2 had 98.82% similarity to Phomopsis sp. 32PG/F. Conclusion: Six isolates of endophytic fungi were obtained, Y1; Y2; Y3; Y4; Y5; and Y6. Four iso¬late the ability of to degrade CMC and three isolate the ability for KCN degrade. Isolate Y2 is the isolate with the best activity for cellulase and cyanidase degrading enzymes, namely 2.99 U/ml and 2.19 U/ml. After molecular identification using 18S rRNA, isolate Y2 had 98.82% similarity to Phomopsis sp. 32PG/F. [J Adv Vet Anim Res 2025; 12(1.000): 169-178]http://www.ejmanager.com/fulltextpdf.php?mno=220864cellulose; endophytic fungus; identification; isolation; screening |
| spellingShingle | Yetti Marlida Husmaini Husmaini Ahadiyah Yuniza Lili Anggraini Wulansih Dwi Astuti Ridho Kurniawan Rusli Hera Dwi Triani Gusri Yanti. Isolation, screening, and molecular identification of endopytic fungus producing cellulose and cyanide degrading enzyme its application for waste cassava. Journal of Advanced Veterinary and Animal Research cellulose; endophytic fungus; identification; isolation; screening |
| title | Isolation, screening, and molecular identification of endopytic fungus producing cellulose and cyanide degrading enzyme its application for waste cassava. |
| title_full | Isolation, screening, and molecular identification of endopytic fungus producing cellulose and cyanide degrading enzyme its application for waste cassava. |
| title_fullStr | Isolation, screening, and molecular identification of endopytic fungus producing cellulose and cyanide degrading enzyme its application for waste cassava. |
| title_full_unstemmed | Isolation, screening, and molecular identification of endopytic fungus producing cellulose and cyanide degrading enzyme its application for waste cassava. |
| title_short | Isolation, screening, and molecular identification of endopytic fungus producing cellulose and cyanide degrading enzyme its application for waste cassava. |
| title_sort | isolation screening and molecular identification of endopytic fungus producing cellulose and cyanide degrading enzyme its application for waste cassava |
| topic | cellulose; endophytic fungus; identification; isolation; screening |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=220864 |
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