Investigation of the relationship between virulence factors and genotype of Candida spp. isolated from blood cultures
Introduction: The aim of study was to investigate the virulence factors of phospholipase, proteinase, esterase production and biofilm formation in Candida species isolated from patients with candidemia, and to assess their relationship with Candida genotypes derived after repetitive sequence-based p...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2015-08-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/5359 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849313618395398144 |
|---|---|
| author | Fatma Mutlu Sariguzel Elife Berk Ayse Nedret Koc Hafize Sav Gonca Demir |
| author_facet | Fatma Mutlu Sariguzel Elife Berk Ayse Nedret Koc Hafize Sav Gonca Demir |
| author_sort | Fatma Mutlu Sariguzel |
| collection | DOAJ |
| description | Introduction: The aim of study was to investigate the virulence factors of phospholipase, proteinase, esterase production and biofilm formation in Candida species isolated from patients with candidemia, and to assess their relationship with Candida genotypes derived after repetitive sequence-based polymerase chain reaction (rep-PCR) fingerprinting.
Methodology: Fifty-two strains were identified to species level according to conventional methods and sequencing. The DiversiLab system was used for the genotyping. Enzyme activities and biofilm formation were evaluated using microbiological methods.
Results: The 52 strains were identified as follows: 29 C. parapsilosis, 19 C. albicans, 2 C. glabrata, and 2 C. tropicalis. Phospholipase and proteinase activities were observed to have statistically significant differences between C. albicans and non-albicans Candida (NAC) strains (p < 0.05), with C. albicans strains showing higher virulence. Rep-PCR revealed eight major genotypes (A-H).The 19 C. albicans and the 33 non-albicans Candida isolates yielded seven (A-G) and four (A, B, C, H) genotypes, respectively. C. albicans strains were not shown to have a predominant genotype and showed higher phospholipase and proteinase activitiy than did NAC, regardless of genotype. Genotype H (52%) was the predominant genotype for the NAC including 27 C. parapsilosis strains, but the majority of strains showed low virulence.
Conclusions: NAC species were the most common causative agent for candidemia. Genotyping showed low transmission of C. albicans strains, but transmission of C. parapsilosis was high. In candidemia, several Candida virulence factors may be responsible at the same time. However, different genotypes of Candida strains showed different virulence activity.
|
| format | Article |
| id | doaj-art-fd06a0ea10774176920fb886c071fdcb |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2015-08-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-fd06a0ea10774176920fb886c071fdcb2025-08-20T03:52:42ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802015-08-0190810.3855/jidc.5359Investigation of the relationship between virulence factors and genotype of Candida spp. isolated from blood culturesFatma Mutlu Sariguzel0Elife Berk1Ayse Nedret Koc2Hafize Sav3Gonca Demir4Kayseri Education and Research Hospital, Kayseri, TurkeyErciyes University Medical School, Kayseri, TurkeyErciyes University Medical School, Kayseri, TurkeyErciyes University Medical School, Kayseri, TurkeyErciyes University Medical School, Kayseri, TurkeyIntroduction: The aim of study was to investigate the virulence factors of phospholipase, proteinase, esterase production and biofilm formation in Candida species isolated from patients with candidemia, and to assess their relationship with Candida genotypes derived after repetitive sequence-based polymerase chain reaction (rep-PCR) fingerprinting. Methodology: Fifty-two strains were identified to species level according to conventional methods and sequencing. The DiversiLab system was used for the genotyping. Enzyme activities and biofilm formation were evaluated using microbiological methods. Results: The 52 strains were identified as follows: 29 C. parapsilosis, 19 C. albicans, 2 C. glabrata, and 2 C. tropicalis. Phospholipase and proteinase activities were observed to have statistically significant differences between C. albicans and non-albicans Candida (NAC) strains (p < 0.05), with C. albicans strains showing higher virulence. Rep-PCR revealed eight major genotypes (A-H).The 19 C. albicans and the 33 non-albicans Candida isolates yielded seven (A-G) and four (A, B, C, H) genotypes, respectively. C. albicans strains were not shown to have a predominant genotype and showed higher phospholipase and proteinase activitiy than did NAC, regardless of genotype. Genotype H (52%) was the predominant genotype for the NAC including 27 C. parapsilosis strains, but the majority of strains showed low virulence. Conclusions: NAC species were the most common causative agent for candidemia. Genotyping showed low transmission of C. albicans strains, but transmission of C. parapsilosis was high. In candidemia, several Candida virulence factors may be responsible at the same time. However, different genotypes of Candida strains showed different virulence activity. https://jidc.org/index.php/journal/article/view/5359Candida spp.clonal relationshipgenotypingvirulence factors |
| spellingShingle | Fatma Mutlu Sariguzel Elife Berk Ayse Nedret Koc Hafize Sav Gonca Demir Investigation of the relationship between virulence factors and genotype of Candida spp. isolated from blood cultures Journal of Infection in Developing Countries Candida spp. clonal relationship genotyping virulence factors |
| title | Investigation of the relationship between virulence factors and genotype of Candida spp. isolated from blood cultures |
| title_full | Investigation of the relationship between virulence factors and genotype of Candida spp. isolated from blood cultures |
| title_fullStr | Investigation of the relationship between virulence factors and genotype of Candida spp. isolated from blood cultures |
| title_full_unstemmed | Investigation of the relationship between virulence factors and genotype of Candida spp. isolated from blood cultures |
| title_short | Investigation of the relationship between virulence factors and genotype of Candida spp. isolated from blood cultures |
| title_sort | investigation of the relationship between virulence factors and genotype of candida spp isolated from blood cultures |
| topic | Candida spp. clonal relationship genotyping virulence factors |
| url | https://jidc.org/index.php/journal/article/view/5359 |
| work_keys_str_mv | AT fatmamutlusariguzel investigationoftherelationshipbetweenvirulencefactorsandgenotypeofcandidasppisolatedfrombloodcultures AT elifeberk investigationoftherelationshipbetweenvirulencefactorsandgenotypeofcandidasppisolatedfrombloodcultures AT aysenedretkoc investigationoftherelationshipbetweenvirulencefactorsandgenotypeofcandidasppisolatedfrombloodcultures AT hafizesav investigationoftherelationshipbetweenvirulencefactorsandgenotypeofcandidasppisolatedfrombloodcultures AT goncademir investigationoftherelationshipbetweenvirulencefactorsandgenotypeofcandidasppisolatedfrombloodcultures |