Transcriptomic profile of microglia following inflammation-sensitized hypoxic-ischemic brain injury in neonatal rats suggests strong contribution to neutrophil chemotaxis and activation
Abstract Background Inflammation-sensitized hypoxic-ischemic brain injury significantly contributes to neonatal mortality as affected neonates do not benefit from standard cooling treatments. To get further insight into inflammatory responses involved, we experimentally investigated the immune respo...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-07-01
|
| Series: | Journal of Neuroinflammation |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12974-025-03516-1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849234585682968576 |
|---|---|
| author | Anna-Sophie Bremer Nico Henschel Hannah Burkard Maria Eugenia Bernis Thomas Ulas Hemmen Sabir |
| author_facet | Anna-Sophie Bremer Nico Henschel Hannah Burkard Maria Eugenia Bernis Thomas Ulas Hemmen Sabir |
| author_sort | Anna-Sophie Bremer |
| collection | DOAJ |
| description | Abstract Background Inflammation-sensitized hypoxic-ischemic brain injury significantly contributes to neonatal mortality as affected neonates do not benefit from standard cooling treatments. To get further insight into inflammatory responses involved, we experimentally investigated the immune response of microglia in an inflammation-sensitized neonatal hypoxia-ischemia (HI) model. Results Transcriptomic analysis of microglia isolated from brains following inflammation-sensitized HI brain injury revealed a strong upregulation of leukocyte recruitment and pro-inflammatory markers. Specifically, markers associated with neutrophil-mediated immune responses and chemotaxis were upregulated in the inflammation-sensitized HI group compared to the non-inflammation-sensitized HI and control groups. Serpine 1 and Selp could be identified as specifically upregulated markers indicating an acute inflammatory condition before HI injury. Conclusion Our study revealed preliminary data about a microglia population which is primed to recruit peripheral neutrophils to infiltrate the brain and mediate neutrophil immune response. We showed a contribution to neutrophil activation in case of inflammation following HI in the brain. Targeting microglia-mediated neutrophil recruitment can indicate a possible treatment approach in case of inflammation-sensitized HI brain injury. |
| format | Article |
| id | doaj-art-fcee4e7a6792443088d124a8ce3ea044 |
| institution | Kabale University |
| issn | 1742-2094 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Neuroinflammation |
| spelling | doaj-art-fcee4e7a6792443088d124a8ce3ea0442025-08-20T04:03:06ZengBMCJournal of Neuroinflammation1742-20942025-07-0122111310.1186/s12974-025-03516-1Transcriptomic profile of microglia following inflammation-sensitized hypoxic-ischemic brain injury in neonatal rats suggests strong contribution to neutrophil chemotaxis and activationAnna-Sophie Bremer0Nico Henschel1Hannah Burkard2Maria Eugenia Bernis3Thomas Ulas4Hemmen Sabir5Department of Neonatology and Pediatric Intensive Care, Children’s Hospital, University of BonnGerman Center for Neurodegenerative Disease (DZNE)Department of Neonatology and Pediatric Intensive Care, Children’s Hospital, University of BonnDepartment of Neonatology and Pediatric Intensive Care, Children’s Hospital, University of BonnGerman Center for Neurodegenerative Disease (DZNE)Department of Neonatology and Pediatric Intensive Care, Children’s Hospital, University of BonnAbstract Background Inflammation-sensitized hypoxic-ischemic brain injury significantly contributes to neonatal mortality as affected neonates do not benefit from standard cooling treatments. To get further insight into inflammatory responses involved, we experimentally investigated the immune response of microglia in an inflammation-sensitized neonatal hypoxia-ischemia (HI) model. Results Transcriptomic analysis of microglia isolated from brains following inflammation-sensitized HI brain injury revealed a strong upregulation of leukocyte recruitment and pro-inflammatory markers. Specifically, markers associated with neutrophil-mediated immune responses and chemotaxis were upregulated in the inflammation-sensitized HI group compared to the non-inflammation-sensitized HI and control groups. Serpine 1 and Selp could be identified as specifically upregulated markers indicating an acute inflammatory condition before HI injury. Conclusion Our study revealed preliminary data about a microglia population which is primed to recruit peripheral neutrophils to infiltrate the brain and mediate neutrophil immune response. We showed a contribution to neutrophil activation in case of inflammation following HI in the brain. Targeting microglia-mediated neutrophil recruitment can indicate a possible treatment approach in case of inflammation-sensitized HI brain injury.https://doi.org/10.1186/s12974-025-03516-1Neonatal Hypoxia-IschemiaBrain inflammationMicroglia primingNeutrophil recruitmentTranscriptomic analysis |
| spellingShingle | Anna-Sophie Bremer Nico Henschel Hannah Burkard Maria Eugenia Bernis Thomas Ulas Hemmen Sabir Transcriptomic profile of microglia following inflammation-sensitized hypoxic-ischemic brain injury in neonatal rats suggests strong contribution to neutrophil chemotaxis and activation Journal of Neuroinflammation Neonatal Hypoxia-Ischemia Brain inflammation Microglia priming Neutrophil recruitment Transcriptomic analysis |
| title | Transcriptomic profile of microglia following inflammation-sensitized hypoxic-ischemic brain injury in neonatal rats suggests strong contribution to neutrophil chemotaxis and activation |
| title_full | Transcriptomic profile of microglia following inflammation-sensitized hypoxic-ischemic brain injury in neonatal rats suggests strong contribution to neutrophil chemotaxis and activation |
| title_fullStr | Transcriptomic profile of microglia following inflammation-sensitized hypoxic-ischemic brain injury in neonatal rats suggests strong contribution to neutrophil chemotaxis and activation |
| title_full_unstemmed | Transcriptomic profile of microglia following inflammation-sensitized hypoxic-ischemic brain injury in neonatal rats suggests strong contribution to neutrophil chemotaxis and activation |
| title_short | Transcriptomic profile of microglia following inflammation-sensitized hypoxic-ischemic brain injury in neonatal rats suggests strong contribution to neutrophil chemotaxis and activation |
| title_sort | transcriptomic profile of microglia following inflammation sensitized hypoxic ischemic brain injury in neonatal rats suggests strong contribution to neutrophil chemotaxis and activation |
| topic | Neonatal Hypoxia-Ischemia Brain inflammation Microglia priming Neutrophil recruitment Transcriptomic analysis |
| url | https://doi.org/10.1186/s12974-025-03516-1 |
| work_keys_str_mv | AT annasophiebremer transcriptomicprofileofmicrogliafollowinginflammationsensitizedhypoxicischemicbraininjuryinneonatalratssuggestsstrongcontributiontoneutrophilchemotaxisandactivation AT nicohenschel transcriptomicprofileofmicrogliafollowinginflammationsensitizedhypoxicischemicbraininjuryinneonatalratssuggestsstrongcontributiontoneutrophilchemotaxisandactivation AT hannahburkard transcriptomicprofileofmicrogliafollowinginflammationsensitizedhypoxicischemicbraininjuryinneonatalratssuggestsstrongcontributiontoneutrophilchemotaxisandactivation AT mariaeugeniabernis transcriptomicprofileofmicrogliafollowinginflammationsensitizedhypoxicischemicbraininjuryinneonatalratssuggestsstrongcontributiontoneutrophilchemotaxisandactivation AT thomasulas transcriptomicprofileofmicrogliafollowinginflammationsensitizedhypoxicischemicbraininjuryinneonatalratssuggestsstrongcontributiontoneutrophilchemotaxisandactivation AT hemmensabir transcriptomicprofileofmicrogliafollowinginflammationsensitizedhypoxicischemicbraininjuryinneonatalratssuggestsstrongcontributiontoneutrophilchemotaxisandactivation |