Dietary effect of Galega orientalis Lam. flavonoid extract on broilers’ growth performance, antioxidant capacity, immune function, and bone metabolism

This study aimed to evaluate the impact of dietary Galega orientalis Lam. flavonoid extract (GOLFE) on production performance, biological markers, and bone health of white-feather broilers. A total of 540 one-day-old Cobb broilers were randomly assigned to six dietary groups: a negative control grou...

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Main Authors: Nana I. Tchana, Huiyan Zhang, Yinchuan Pan, Shiyi Wang, Dieudonné M. Dansou, Xiaoxuan Xia, Qingyu Zhao, Chaohua Tang, Junmin Zhang
Format: Article
Language:English
Published: Elsevier 2025-05-01
Series:Poultry Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S0032579125003001
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author Nana I. Tchana
Huiyan Zhang
Yinchuan Pan
Shiyi Wang
Dieudonné M. Dansou
Xiaoxuan Xia
Qingyu Zhao
Chaohua Tang
Junmin Zhang
author_facet Nana I. Tchana
Huiyan Zhang
Yinchuan Pan
Shiyi Wang
Dieudonné M. Dansou
Xiaoxuan Xia
Qingyu Zhao
Chaohua Tang
Junmin Zhang
author_sort Nana I. Tchana
collection DOAJ
description This study aimed to evaluate the impact of dietary Galega orientalis Lam. flavonoid extract (GOLFE) on production performance, biological markers, and bone health of white-feather broilers. A total of 540 one-day-old Cobb broilers were randomly assigned to six dietary groups: a negative control group (without additives; G0), four nutritional additive groups supplemented with GOLFE (G1:200 mg, G2: 400 mg, G3:800 mg, and G4:1600 mg), and a positive control group (supplemented with chlortetracycline; G5:100 mg). Each group consisted of 90 birds (6 replicates with 15 birds each) and the feeding trial lasted 42 days. Growth performance and blood profiles were assessed during two phases (days 1–21 and 22–42), while bone health, serum biochemical, antioxidant, and immune parameters were evaluated in the second phase (22–42 days). The results demonstrated that GOLFE supplementation significantly reduced mortality in birds, improved feed conversion ratio, and reduced feed intake (P < 0.05) without significant effect on body weight (P > 0.05). GOLFE also led to a reduction in white blood cell counts (P < 0.05). Serum biochemical and antioxidant analyses revealed substantial improvements in calcium (Ca) and phosphorus (P) concentrations (P < 0.05), alongside a decrease in MDA biomarkers, with an increase in SOD, CAT, and TAOC content (P < 0.05). Significant decrease of pro-inflammatory cytokines (P < 0.05), including interleukins (IL): IL-1β, IL-2, IL-4, IL-6, and IL-8 were also noticed. Additionally, growth plate histomorphometry indicated that GOLFE enhanced the microstructure of bone tissue, and tibia bone characteristics such as weight, length, strength (P < 0.05), and mineral content (Ca and P), (P < 0.05) were also improved. These findings suggest that GOLFE supplementation may promote better growth performance and overall health in broilers, positioning it as a potential natural alternative to antibiotics in poultry production.
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spelling doaj-art-fcec418d898f472a8a547f36af5d212e2025-08-20T02:31:00ZengElsevierPoultry Science0032-57912025-05-01104510506110.1016/j.psj.2025.105061Dietary effect of Galega orientalis Lam. flavonoid extract on broilers’ growth performance, antioxidant capacity, immune function, and bone metabolismNana I. Tchana0Huiyan Zhang1Yinchuan Pan2Shiyi Wang3Dieudonné M. Dansou4Xiaoxuan Xia5Qingyu Zhao6Chaohua Tang7Junmin Zhang8State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, ChinaCorresponding author.; State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, ChinaThis study aimed to evaluate the impact of dietary Galega orientalis Lam. flavonoid extract (GOLFE) on production performance, biological markers, and bone health of white-feather broilers. A total of 540 one-day-old Cobb broilers were randomly assigned to six dietary groups: a negative control group (without additives; G0), four nutritional additive groups supplemented with GOLFE (G1:200 mg, G2: 400 mg, G3:800 mg, and G4:1600 mg), and a positive control group (supplemented with chlortetracycline; G5:100 mg). Each group consisted of 90 birds (6 replicates with 15 birds each) and the feeding trial lasted 42 days. Growth performance and blood profiles were assessed during two phases (days 1–21 and 22–42), while bone health, serum biochemical, antioxidant, and immune parameters were evaluated in the second phase (22–42 days). The results demonstrated that GOLFE supplementation significantly reduced mortality in birds, improved feed conversion ratio, and reduced feed intake (P < 0.05) without significant effect on body weight (P > 0.05). GOLFE also led to a reduction in white blood cell counts (P < 0.05). Serum biochemical and antioxidant analyses revealed substantial improvements in calcium (Ca) and phosphorus (P) concentrations (P < 0.05), alongside a decrease in MDA biomarkers, with an increase in SOD, CAT, and TAOC content (P < 0.05). Significant decrease of pro-inflammatory cytokines (P < 0.05), including interleukins (IL): IL-1β, IL-2, IL-4, IL-6, and IL-8 were also noticed. Additionally, growth plate histomorphometry indicated that GOLFE enhanced the microstructure of bone tissue, and tibia bone characteristics such as weight, length, strength (P < 0.05), and mineral content (Ca and P), (P < 0.05) were also improved. These findings suggest that GOLFE supplementation may promote better growth performance and overall health in broilers, positioning it as a potential natural alternative to antibiotics in poultry production.http://www.sciencedirect.com/science/article/pii/S0032579125003001Antibiotic alternativeBroiler performanceFlavonoid extractGalega orientalis Lam.Bone quality
spellingShingle Nana I. Tchana
Huiyan Zhang
Yinchuan Pan
Shiyi Wang
Dieudonné M. Dansou
Xiaoxuan Xia
Qingyu Zhao
Chaohua Tang
Junmin Zhang
Dietary effect of Galega orientalis Lam. flavonoid extract on broilers’ growth performance, antioxidant capacity, immune function, and bone metabolism
Poultry Science
Antibiotic alternative
Broiler performance
Flavonoid extract
Galega orientalis Lam.
Bone quality
title Dietary effect of Galega orientalis Lam. flavonoid extract on broilers’ growth performance, antioxidant capacity, immune function, and bone metabolism
title_full Dietary effect of Galega orientalis Lam. flavonoid extract on broilers’ growth performance, antioxidant capacity, immune function, and bone metabolism
title_fullStr Dietary effect of Galega orientalis Lam. flavonoid extract on broilers’ growth performance, antioxidant capacity, immune function, and bone metabolism
title_full_unstemmed Dietary effect of Galega orientalis Lam. flavonoid extract on broilers’ growth performance, antioxidant capacity, immune function, and bone metabolism
title_short Dietary effect of Galega orientalis Lam. flavonoid extract on broilers’ growth performance, antioxidant capacity, immune function, and bone metabolism
title_sort dietary effect of galega orientalis lam flavonoid extract on broilers growth performance antioxidant capacity immune function and bone metabolism
topic Antibiotic alternative
Broiler performance
Flavonoid extract
Galega orientalis Lam.
Bone quality
url http://www.sciencedirect.com/science/article/pii/S0032579125003001
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