Histological ovarian features and hormonal determinations in assigned females at birth transgender individuals according to different testosterone preparations

IntroductionDistinct androgen formulations have been used as gender-affirming hormone treatment, but little is known about the specific changes that may occur in the ovary depending on the testosterone preparation used. The study aims to evaluate the histological modifications of the ovarian tissue...

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Main Authors: Aina Borrás, Yasmina Barral, Francesc Fabregues, Gemma Casals, Mireia Mora, Aida Orois, Marta Méndez, Adela Saco, Anna Goday, Dolors Manau
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-10-01
Series:Frontiers in Endocrinology
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Online Access:https://www.frontiersin.org/articles/10.3389/fendo.2024.1458846/full
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author Aina Borrás
Aina Borrás
Yasmina Barral
Francesc Fabregues
Francesc Fabregues
Gemma Casals
Gemma Casals
Mireia Mora
Aida Orois
Marta Méndez
Adela Saco
Anna Goday
Dolors Manau
Dolors Manau
author_facet Aina Borrás
Aina Borrás
Yasmina Barral
Francesc Fabregues
Francesc Fabregues
Gemma Casals
Gemma Casals
Mireia Mora
Aida Orois
Marta Méndez
Adela Saco
Anna Goday
Dolors Manau
Dolors Manau
author_sort Aina Borrás
collection DOAJ
description IntroductionDistinct androgen formulations have been used as gender-affirming hormone treatment, but little is known about the specific changes that may occur in the ovary depending on the testosterone preparation used. The study aims to evaluate the histological modifications of the ovarian tissue and the hormonal changes after gender-affirming surgery based on the testosterone preparation employed, such as testosterone cypionate or undecanoate.DesignUnicenter transversal cohort study.Materials and methodsSixty transmasculine persons before and after gender-affirming surgery. A histological examination of the ovaries was conducted, including the follicular population and the characterization of the ovarian stroma. Hormonal status (testosterone, estradiol, FSH, and LH) were also assessed before and after the procedure.ResultsThe median age of participants was similar between the two groups (27.9 vs. 26.7 years, p = 0.27). There were no differences in all hormonal determinations before gender-affirming surgery between the groups. After surgery, FSH levels increased significantly, especially in the testosterone undecanoate group compared to the cypionate group (72.3 vs. 38.3 U/L, p = 0.02), consistent with LH determinations (43.0 vs. 23.4 U/L, p = 0.02). However, no regimen modification was required for any individual. No statistical differences were observed in any parameter concerning the follicular population, nor were there any variances in the thickness of the tunica albuginea (p = 0.85) or the proportion of luteinized stromal cells. Nevertheless, there was a tendency toward increased luteinization in the testosterone cypionate group (88.2% vs. 76.9%, p > 0.05).ConclusionsIn a cohort of transmasculine individuals using different androgen preparations, histological analysis of ovarian tissue revealed comparable findings. Both groups exhibited similar follicular populations and comparable modifications in stromal tissue. However, significant differences were observed in hormonal profiles, although no modification in testosterone dosage was needed.
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spelling doaj-art-fcdacb84402d42bcbfbc12ba720f78bc2025-08-20T02:11:17ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922024-10-011510.3389/fendo.2024.14588461458846Histological ovarian features and hormonal determinations in assigned females at birth transgender individuals according to different testosterone preparationsAina Borrás0Aina Borrás1Yasmina Barral2Francesc Fabregues3Francesc Fabregues4Gemma Casals5Gemma Casals6Mireia Mora7Aida Orois8Marta Méndez9Adela Saco10Anna Goday11Dolors Manau12Dolors Manau13Clinical Institute of Gynecology, Obstetrics, and Neonatology, Hospital Clinic, Barcelona, SpainInstitut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Gynecology, Human Reproduction and Women’s Health Group, Barcelona, SpainClinical Institute of Gynecology, Obstetrics, and Neonatology, Hospital Clinic, Barcelona, SpainClinical Institute of Gynecology, Obstetrics, and Neonatology, Hospital Clinic, Barcelona, SpainInstitut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Gynecology, Human Reproduction and Women’s Health Group, Barcelona, SpainClinical Institute of Gynecology, Obstetrics, and Neonatology, Hospital Clinic, Barcelona, SpainInstitut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Gynecology, Human Reproduction and Women’s Health Group, Barcelona, SpainEndocrinology and Nutrition Department, Hospital Clínic, Universitat de Barcelona, Centro de Investigación Biomédica en Red. Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Barcelona, SpainEndocrinology and Nutrition Department, Hospital Clínic, Universitat de Barcelona, Centro de Investigación Biomédica en Red. Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Barcelona, SpainClinical Institute of Gynecology, Obstetrics, and Neonatology, Hospital Clinic, Barcelona, SpainDeparment of Pathology, Hospital Clinic, University of Barcelona, Barcelona, SpainClinical Institute of Gynecology, Obstetrics, and Neonatology, Hospital Clinic, Barcelona, SpainClinical Institute of Gynecology, Obstetrics, and Neonatology, Hospital Clinic, Barcelona, SpainInstitut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Gynecology, Human Reproduction and Women’s Health Group, Barcelona, SpainIntroductionDistinct androgen formulations have been used as gender-affirming hormone treatment, but little is known about the specific changes that may occur in the ovary depending on the testosterone preparation used. The study aims to evaluate the histological modifications of the ovarian tissue and the hormonal changes after gender-affirming surgery based on the testosterone preparation employed, such as testosterone cypionate or undecanoate.DesignUnicenter transversal cohort study.Materials and methodsSixty transmasculine persons before and after gender-affirming surgery. A histological examination of the ovaries was conducted, including the follicular population and the characterization of the ovarian stroma. Hormonal status (testosterone, estradiol, FSH, and LH) were also assessed before and after the procedure.ResultsThe median age of participants was similar between the two groups (27.9 vs. 26.7 years, p = 0.27). There were no differences in all hormonal determinations before gender-affirming surgery between the groups. After surgery, FSH levels increased significantly, especially in the testosterone undecanoate group compared to the cypionate group (72.3 vs. 38.3 U/L, p = 0.02), consistent with LH determinations (43.0 vs. 23.4 U/L, p = 0.02). However, no regimen modification was required for any individual. No statistical differences were observed in any parameter concerning the follicular population, nor were there any variances in the thickness of the tunica albuginea (p = 0.85) or the proportion of luteinized stromal cells. Nevertheless, there was a tendency toward increased luteinization in the testosterone cypionate group (88.2% vs. 76.9%, p > 0.05).ConclusionsIn a cohort of transmasculine individuals using different androgen preparations, histological analysis of ovarian tissue revealed comparable findings. Both groups exhibited similar follicular populations and comparable modifications in stromal tissue. However, significant differences were observed in hormonal profiles, although no modification in testosterone dosage was needed.https://www.frontiersin.org/articles/10.3389/fendo.2024.1458846/fullhormone treatmenthistological ovarian featuresdifferent testosterone preparationstransgenderhormonal profile
spellingShingle Aina Borrás
Aina Borrás
Yasmina Barral
Francesc Fabregues
Francesc Fabregues
Gemma Casals
Gemma Casals
Mireia Mora
Aida Orois
Marta Méndez
Adela Saco
Anna Goday
Dolors Manau
Dolors Manau
Histological ovarian features and hormonal determinations in assigned females at birth transgender individuals according to different testosterone preparations
Frontiers in Endocrinology
hormone treatment
histological ovarian features
different testosterone preparations
transgender
hormonal profile
title Histological ovarian features and hormonal determinations in assigned females at birth transgender individuals according to different testosterone preparations
title_full Histological ovarian features and hormonal determinations in assigned females at birth transgender individuals according to different testosterone preparations
title_fullStr Histological ovarian features and hormonal determinations in assigned females at birth transgender individuals according to different testosterone preparations
title_full_unstemmed Histological ovarian features and hormonal determinations in assigned females at birth transgender individuals according to different testosterone preparations
title_short Histological ovarian features and hormonal determinations in assigned females at birth transgender individuals according to different testosterone preparations
title_sort histological ovarian features and hormonal determinations in assigned females at birth transgender individuals according to different testosterone preparations
topic hormone treatment
histological ovarian features
different testosterone preparations
transgender
hormonal profile
url https://www.frontiersin.org/articles/10.3389/fendo.2024.1458846/full
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