STUDY OF THE STABILITY IN REAL TIME OF CRYOPRESERVED STRAIN BANKS
The stability in real time of four strains cryopreserved in 10% v/v of glycerol was evaluated during a 6-month period. The strains studied were Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus niger. The Master Cell Bank (MCB), cryopreserved at -70ºC and -20ºC, was activ...
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Pontificia Universidad Javeriana
2004-12-01
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| Series: | Universitas Scientiarum |
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| Online Access: | https://docs.google.com/folder/d/0B_IdlsctkRrxVXFtcGt3eHgxZGM/edit?usp=sharing&pli=1&docId=0B_IdlsctkRrxd3ZBa0puWHZLaDQ |
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| _version_ | 1850162151872790528 |
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| author | Rodrigo Antonio Meza Andrea Fernanda Monro Marcela Mercado Raúl Alberto Poutou Patricia Rodríguez Aura Marina Pedroza |
| author_facet | Rodrigo Antonio Meza Andrea Fernanda Monro Marcela Mercado Raúl Alberto Poutou Patricia Rodríguez Aura Marina Pedroza |
| author_sort | Rodrigo Antonio Meza |
| collection | DOAJ |
| description | The stability in real time of four strains cryopreserved in 10% v/v of glycerol was evaluated during a 6-month period. The strains studied were Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus niger. The Master Cell Bank (MCB), cryopreserved at -70ºC and -20ºC, was activated using two thawing protocols, a fast one (F) and a slow one (S). A better cell recovery was achieved with the -70ºC (F) protocol reaching a viability for Escherichia coli of 97.6% in the first 48 hours (p: 7.2x10-4). The viability was retained in the 4 th (p: 1.5 x10 -4), 5 th (p: 4.6 x10-3) and 6 th months (p: 1.9 x10-2). Bacillus subtilis retained a viability of 92.5% after the 2nd (p: 4.7x10-4), 4th (p: 1.76x10-1), 5th (p: 3.4x10-5) and 6th months (p: 3x10-2). Saccharomyces cerevisiae demonstrated a viability of 95% in the second month (p:9x10-3) and for Aspergillus niger, the viability was 94.8%, in the first 48 hours (p: 7.2 x10-4) and after the 4th month (p: 2.79x10-2). With the other protocols, -20ºC F and S, some changes in viability were observed due probably to the formation of eutectic mixtures, nucleation and re-crystallization processes. The Master Bank microbial purity was maintained at 100% during the time of the study. |
| format | Article |
| id | doaj-art-fcd5d63cbf0147aa9bf8304c0fcef3f6 |
| institution | OA Journals |
| issn | 0122-7483 2027-1352 |
| language | English |
| publishDate | 2004-12-01 |
| publisher | Pontificia Universidad Javeriana |
| record_format | Article |
| series | Universitas Scientiarum |
| spelling | doaj-art-fcd5d63cbf0147aa9bf8304c0fcef3f62025-08-20T02:22:38ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522004-12-01923542STUDY OF THE STABILITY IN REAL TIME OF CRYOPRESERVED STRAIN BANKSRodrigo Antonio MezaAndrea Fernanda MonroMarcela MercadoRaúl Alberto PoutouPatricia RodríguezAura Marina PedrozaThe stability in real time of four strains cryopreserved in 10% v/v of glycerol was evaluated during a 6-month period. The strains studied were Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus niger. The Master Cell Bank (MCB), cryopreserved at -70ºC and -20ºC, was activated using two thawing protocols, a fast one (F) and a slow one (S). A better cell recovery was achieved with the -70ºC (F) protocol reaching a viability for Escherichia coli of 97.6% in the first 48 hours (p: 7.2x10-4). The viability was retained in the 4 th (p: 1.5 x10 -4), 5 th (p: 4.6 x10-3) and 6 th months (p: 1.9 x10-2). Bacillus subtilis retained a viability of 92.5% after the 2nd (p: 4.7x10-4), 4th (p: 1.76x10-1), 5th (p: 3.4x10-5) and 6th months (p: 3x10-2). Saccharomyces cerevisiae demonstrated a viability of 95% in the second month (p:9x10-3) and for Aspergillus niger, the viability was 94.8%, in the first 48 hours (p: 7.2 x10-4) and after the 4th month (p: 2.79x10-2). With the other protocols, -20ºC F and S, some changes in viability were observed due probably to the formation of eutectic mixtures, nucleation and re-crystallization processes. The Master Bank microbial purity was maintained at 100% during the time of the study.https://docs.google.com/folder/d/0B_IdlsctkRrxVXFtcGt3eHgxZGM/edit?usp=sharing&pli=1&docId=0B_IdlsctkRrxd3ZBa0puWHZLaDQCryopreservationeutecticthawingfreezing. |
| spellingShingle | Rodrigo Antonio Meza Andrea Fernanda Monro Marcela Mercado Raúl Alberto Poutou Patricia Rodríguez Aura Marina Pedroza STUDY OF THE STABILITY IN REAL TIME OF CRYOPRESERVED STRAIN BANKS Universitas Scientiarum Cryopreservation eutectic thawing freezing. |
| title | STUDY OF THE STABILITY IN REAL TIME OF CRYOPRESERVED STRAIN BANKS |
| title_full | STUDY OF THE STABILITY IN REAL TIME OF CRYOPRESERVED STRAIN BANKS |
| title_fullStr | STUDY OF THE STABILITY IN REAL TIME OF CRYOPRESERVED STRAIN BANKS |
| title_full_unstemmed | STUDY OF THE STABILITY IN REAL TIME OF CRYOPRESERVED STRAIN BANKS |
| title_short | STUDY OF THE STABILITY IN REAL TIME OF CRYOPRESERVED STRAIN BANKS |
| title_sort | study of the stability in real time of cryopreserved strain banks |
| topic | Cryopreservation eutectic thawing freezing. |
| url | https://docs.google.com/folder/d/0B_IdlsctkRrxVXFtcGt3eHgxZGM/edit?usp=sharing&pli=1&docId=0B_IdlsctkRrxd3ZBa0puWHZLaDQ |
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