PCYT2 mediates ovarian epithelial cancer metastasis by regulating cell membrane fluidity through the AMPK/FOXO1 signalling pathway
Abstract This study investigates the role of phosphatidylethanolamine cytidylyltransferase 2 (PCYT2) in ovarian epithelial cancer, specifically examining its effects on cell migration and membrane fluidity. To achieve this, we will examine how the AMPK and FOXO1 pathways regulate these processes. Ou...
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Nature Portfolio
2025-04-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-96405-9 |
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| author | Hailin Chen Yulian Xie Fei Xia Wengong Wei Ru Zhang Chenwu Liu Yinong Shi Qi Xu |
| author_facet | Hailin Chen Yulian Xie Fei Xia Wengong Wei Ru Zhang Chenwu Liu Yinong Shi Qi Xu |
| author_sort | Hailin Chen |
| collection | DOAJ |
| description | Abstract This study investigates the role of phosphatidylethanolamine cytidylyltransferase 2 (PCYT2) in ovarian epithelial cancer, specifically examining its effects on cell migration and membrane fluidity. To achieve this, we will examine how the AMPK and FOXO1 pathways regulate these processes. Our analysis revealed a significant upregulation of PCYT2 expression in metastatic ovarian cancer tissues compared to primary cancer sites, which correlates with altered membrane fluidity. Our data indicate that PCYT2 is essential for modulating the invasive characteristics of ovarian cancer cells. It does this by regulating the expression levels of AMPK and FOXO1, suggesting its role as an upstream regulator in this signaling pathway. Experiments that either inhibit or enhance PCYT2 activity suggest that it may influence cancer cell infiltration by changing membrane fluidity. These findings provide valuable insights into the molecular mechanisms of ovarian cancer metastasis and highlight PCYT2 as a promising therapeutic target. Future research should validate these findings in larger cohort studies, and also explore the therapeutic potential of targeting PCYT2 in ovarian cancer treatment. In conclusion, although there have been substantial advancements in ovarian cancer therapies, the intricate nature of its metastatic behavior remains a major challenge. Our research clearly demonstrates the critical role of PCYT2, urging the scientific community to deepen their understanding of its involvement in cancer progression and to develop innovative treatment strategies. |
| format | Article |
| id | doaj-art-fca8e55adf6e400a8d0775f4ca203a43 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-fca8e55adf6e400a8d0775f4ca203a432025-08-20T02:11:46ZengNature PortfolioScientific Reports2045-23222025-04-0115111210.1038/s41598-025-96405-9PCYT2 mediates ovarian epithelial cancer metastasis by regulating cell membrane fluidity through the AMPK/FOXO1 signalling pathwayHailin Chen0Yulian Xie1Fei Xia2Wengong Wei3Ru Zhang4Chenwu Liu5Yinong Shi6Qi Xu7Department of Reproductive Medicine Center, The First Affiliated Hospital of Soochow UniversityDepartment of Gynecology, Qingpu Branch, Zhongshan Hospital, Fudan UniversityDepartment of Reproductive Medicine Center, The First Affiliated Hospital of Soochow UniversityDepartment of Gynecology, Qingpu Branch, Zhongshan Hospital, Fudan UniversityDepartment of Gynecology, Qingpu Branch, Zhongshan Hospital, Fudan UniversityDepartment of Gynecology, Qingpu Branch, Zhongshan Hospital, Fudan UniversityDepartment of Gynecology, Qingpu Branch, Zhongshan Hospital, Fudan UniversityDepartment of Gynecology, Qingpu Branch, Zhongshan Hospital, Fudan UniversityAbstract This study investigates the role of phosphatidylethanolamine cytidylyltransferase 2 (PCYT2) in ovarian epithelial cancer, specifically examining its effects on cell migration and membrane fluidity. To achieve this, we will examine how the AMPK and FOXO1 pathways regulate these processes. Our analysis revealed a significant upregulation of PCYT2 expression in metastatic ovarian cancer tissues compared to primary cancer sites, which correlates with altered membrane fluidity. Our data indicate that PCYT2 is essential for modulating the invasive characteristics of ovarian cancer cells. It does this by regulating the expression levels of AMPK and FOXO1, suggesting its role as an upstream regulator in this signaling pathway. Experiments that either inhibit or enhance PCYT2 activity suggest that it may influence cancer cell infiltration by changing membrane fluidity. These findings provide valuable insights into the molecular mechanisms of ovarian cancer metastasis and highlight PCYT2 as a promising therapeutic target. Future research should validate these findings in larger cohort studies, and also explore the therapeutic potential of targeting PCYT2 in ovarian cancer treatment. In conclusion, although there have been substantial advancements in ovarian cancer therapies, the intricate nature of its metastatic behavior remains a major challenge. Our research clearly demonstrates the critical role of PCYT2, urging the scientific community to deepen their understanding of its involvement in cancer progression and to develop innovative treatment strategies.https://doi.org/10.1038/s41598-025-96405-9Ovarian epithelial cancerPCYT2AMPK/FOXO1 signalling pathwayCell membrane fluidityMetastasis. |
| spellingShingle | Hailin Chen Yulian Xie Fei Xia Wengong Wei Ru Zhang Chenwu Liu Yinong Shi Qi Xu PCYT2 mediates ovarian epithelial cancer metastasis by regulating cell membrane fluidity through the AMPK/FOXO1 signalling pathway Scientific Reports Ovarian epithelial cancer PCYT2 AMPK/FOXO1 signalling pathway Cell membrane fluidity Metastasis. |
| title | PCYT2 mediates ovarian epithelial cancer metastasis by regulating cell membrane fluidity through the AMPK/FOXO1 signalling pathway |
| title_full | PCYT2 mediates ovarian epithelial cancer metastasis by regulating cell membrane fluidity through the AMPK/FOXO1 signalling pathway |
| title_fullStr | PCYT2 mediates ovarian epithelial cancer metastasis by regulating cell membrane fluidity through the AMPK/FOXO1 signalling pathway |
| title_full_unstemmed | PCYT2 mediates ovarian epithelial cancer metastasis by regulating cell membrane fluidity through the AMPK/FOXO1 signalling pathway |
| title_short | PCYT2 mediates ovarian epithelial cancer metastasis by regulating cell membrane fluidity through the AMPK/FOXO1 signalling pathway |
| title_sort | pcyt2 mediates ovarian epithelial cancer metastasis by regulating cell membrane fluidity through the ampk foxo1 signalling pathway |
| topic | Ovarian epithelial cancer PCYT2 AMPK/FOXO1 signalling pathway Cell membrane fluidity Metastasis. |
| url | https://doi.org/10.1038/s41598-025-96405-9 |
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