Bioluminescence Imaging of Vascular Endothelial Growth Factor Promoter Activity in Murine Mammary Tumorigenesis

Vascular endothelial growth factor (VEGF) is a major inducer of angiogenesis. We generated a transgenic reporter mouse, VEGF-GL, in which an enhanced green fluorescent protein-luciferase fusion protein is expressed under the control of a human VEGF-A promoter. The VEGF-GL mouse exhibited intense bio...

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Main Authors: Shannon L. Faley, Keiko Takahashi, Cornelia E. Crooke, Joshua T. Beckham, Takuya Tomemori, Scott B. Shappell, E. Duco Jansen, Takamune Takahashi
Format: Article
Language:English
Published: SAGE Publishing 2007-09-01
Series:Molecular Imaging
Online Access:https://doi.org/10.2310/7290.2007.00029
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author Shannon L. Faley
Keiko Takahashi
Cornelia E. Crooke
Joshua T. Beckham
Takuya Tomemori
Scott B. Shappell
E. Duco Jansen
Takamune Takahashi
author_facet Shannon L. Faley
Keiko Takahashi
Cornelia E. Crooke
Joshua T. Beckham
Takuya Tomemori
Scott B. Shappell
E. Duco Jansen
Takamune Takahashi
author_sort Shannon L. Faley
collection DOAJ
description Vascular endothelial growth factor (VEGF) is a major inducer of angiogenesis. We generated a transgenic reporter mouse, VEGF-GL, in which an enhanced green fluorescent protein-luciferase fusion protein is expressed under the control of a human VEGF-A promoter. The VEGF-GL mouse exhibited intense bioluminescence throughout the body at 1 week of age. The signals rapidly declined to a relatively low level as the mice grew. The adult VEGF-GL mouse showed restricted bioluminescence to the areas undergoing wound healing. In contrast, the VEGF-GL mice, which were crossed with mouse mammary tumor virus-polyoma virus middle T antigen transgenic mammary tumor mice, exhibited prominent bioluminescence in the tumors, correlating with VEGF transcription. Tumor bioluminescence was observed in the bigenic mice as early as 8 weeks, before tumors were palpable, and the signals increased with tumor growth. In conclusion, the VEGF-GL mouse permits longitudinal and quantitative assessment of VEGF promoter activity in vivo. The model should facilitate understanding of the molecular controls and pathways that regulate VEGF transcription in vivo.
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series Molecular Imaging
spelling doaj-art-fc81167362c9454bb2d95ea9ce6b73252025-01-03T00:10:41ZengSAGE PublishingMolecular Imaging1536-01212007-09-01610.2310/7290.2007.0002910.2310_7290.2007.00029Bioluminescence Imaging of Vascular Endothelial Growth Factor Promoter Activity in Murine Mammary TumorigenesisShannon L. FaleyKeiko TakahashiCornelia E. CrookeJoshua T. BeckhamTakuya TomemoriScott B. ShappellE. Duco JansenTakamune TakahashiVascular endothelial growth factor (VEGF) is a major inducer of angiogenesis. We generated a transgenic reporter mouse, VEGF-GL, in which an enhanced green fluorescent protein-luciferase fusion protein is expressed under the control of a human VEGF-A promoter. The VEGF-GL mouse exhibited intense bioluminescence throughout the body at 1 week of age. The signals rapidly declined to a relatively low level as the mice grew. The adult VEGF-GL mouse showed restricted bioluminescence to the areas undergoing wound healing. In contrast, the VEGF-GL mice, which were crossed with mouse mammary tumor virus-polyoma virus middle T antigen transgenic mammary tumor mice, exhibited prominent bioluminescence in the tumors, correlating with VEGF transcription. Tumor bioluminescence was observed in the bigenic mice as early as 8 weeks, before tumors were palpable, and the signals increased with tumor growth. In conclusion, the VEGF-GL mouse permits longitudinal and quantitative assessment of VEGF promoter activity in vivo. The model should facilitate understanding of the molecular controls and pathways that regulate VEGF transcription in vivo.https://doi.org/10.2310/7290.2007.00029
spellingShingle Shannon L. Faley
Keiko Takahashi
Cornelia E. Crooke
Joshua T. Beckham
Takuya Tomemori
Scott B. Shappell
E. Duco Jansen
Takamune Takahashi
Bioluminescence Imaging of Vascular Endothelial Growth Factor Promoter Activity in Murine Mammary Tumorigenesis
Molecular Imaging
title Bioluminescence Imaging of Vascular Endothelial Growth Factor Promoter Activity in Murine Mammary Tumorigenesis
title_full Bioluminescence Imaging of Vascular Endothelial Growth Factor Promoter Activity in Murine Mammary Tumorigenesis
title_fullStr Bioluminescence Imaging of Vascular Endothelial Growth Factor Promoter Activity in Murine Mammary Tumorigenesis
title_full_unstemmed Bioluminescence Imaging of Vascular Endothelial Growth Factor Promoter Activity in Murine Mammary Tumorigenesis
title_short Bioluminescence Imaging of Vascular Endothelial Growth Factor Promoter Activity in Murine Mammary Tumorigenesis
title_sort bioluminescence imaging of vascular endothelial growth factor promoter activity in murine mammary tumorigenesis
url https://doi.org/10.2310/7290.2007.00029
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