<p><strong>Multiplex-PCR differentiation of two <em>Hyalomma </em>and two <em>Haemaphysalis </em>species (Acari: Ixodidae)</strong></p>
Hyalomma and Haemaphysalis are two most important genera of ticks (Acari: Ixodidae). The rapid and accurate identification of field collected specimens is crucial in faunal works, laboratory assays, anti-tick vaccine experiments, etc. The present study was designed to introduce a rapid and more sen...
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Acarological Society of Iran
2017-04-01
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| Series: | Persian Journal of Acarology |
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| Online Access: | https://www.biotaxa.org/pja/article/view/24392 |
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| author | Asadollah Hosseini Chegeni Mohammad Hassan Kayedi Reza Hosseini Zakkyeh Telmadarraiy |
| author_facet | Asadollah Hosseini Chegeni Mohammad Hassan Kayedi Reza Hosseini Zakkyeh Telmadarraiy |
| author_sort | Asadollah Hosseini Chegeni |
| collection | DOAJ |
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Hyalomma and Haemaphysalis are two most important genera of ticks (Acari: Ixodidae). The rapid and accurate identification of field collected specimens is crucial in faunal works, laboratory assays, anti-tick vaccine experiments, etc. The present study was designed to introduce a rapid and more sensitive method for the differentiation of closely related Hyalomma and Haemaphysalis species namely the pairs Hy. anatolicum-Hy. asiaticum and Ha. punctata-Ha. sulcata, as the main vectors of different animal and human pathogenic agents. Tick specimens were collected from domestic animals in Ardabil, Hormozgan, Khuzestan, Kurdistan, Lorestan, and Mazandaran and identified according to the taxonomic keys. DNA was extracted by the Phenol-Chloroform method. Then, PCR was carried out in a single PCR reaction tube using three pairs designed primers (one forward and two reverse) adapted from the internal transcribe spacer 2 (ITS2) and cytochrome oxidase subunit 1 (COI) genes for Hyalomma and Haemaphysalis, respectively. In the present study, results of Multiplex-PCR revealed that the pairs Hy. anatolicum-H. asiaticum and Ha. punctata-Ha. sulcata could be well differentiated on gel electrophoresis. Morphological misidentification of Hy. anatolicum-Hy. asiaticum and Ha. punctata-Ha. sulcata could be reduced significantly after using Multiplex-PCR.
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| format | Article |
| id | doaj-art-fc7cb8fc6b0142be95ddaa78888bb4a0 |
| institution | OA Journals |
| issn | 2251-8169 |
| language | English |
| publishDate | 2017-04-01 |
| publisher | Acarological Society of Iran |
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| series | Persian Journal of Acarology |
| spelling | doaj-art-fc7cb8fc6b0142be95ddaa78888bb4a02025-08-20T02:29:26ZengAcarological Society of IranPersian Journal of Acarology2251-81692017-04-016210.22073/pja.v6i2.24392<p><strong>Multiplex-PCR differentiation of two <em>Hyalomma </em>and two <em>Haemaphysalis </em>species (Acari: Ixodidae)</strong></p>Asadollah Hosseini Chegeni0Mohammad Hassan Kayedi1Reza Hosseini2Zakkyeh Telmadarraiy3Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranDepartment of Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Lorestan, IranDepartment of Plant Protection, Faculty of Agriculture, University of Guilan, RashtDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Hyalomma and Haemaphysalis are two most important genera of ticks (Acari: Ixodidae). The rapid and accurate identification of field collected specimens is crucial in faunal works, laboratory assays, anti-tick vaccine experiments, etc. The present study was designed to introduce a rapid and more sensitive method for the differentiation of closely related Hyalomma and Haemaphysalis species namely the pairs Hy. anatolicum-Hy. asiaticum and Ha. punctata-Ha. sulcata, as the main vectors of different animal and human pathogenic agents. Tick specimens were collected from domestic animals in Ardabil, Hormozgan, Khuzestan, Kurdistan, Lorestan, and Mazandaran and identified according to the taxonomic keys. DNA was extracted by the Phenol-Chloroform method. Then, PCR was carried out in a single PCR reaction tube using three pairs designed primers (one forward and two reverse) adapted from the internal transcribe spacer 2 (ITS2) and cytochrome oxidase subunit 1 (COI) genes for Hyalomma and Haemaphysalis, respectively. In the present study, results of Multiplex-PCR revealed that the pairs Hy. anatolicum-H. asiaticum and Ha. punctata-Ha. sulcata could be well differentiated on gel electrophoresis. Morphological misidentification of Hy. anatolicum-Hy. asiaticum and Ha. punctata-Ha. sulcata could be reduced significantly after using Multiplex-PCR. https://www.biotaxa.org/pja/article/view/24392Closely related speciesCOIITS2HyalommaHaemaphysalisIdentification |
| spellingShingle | Asadollah Hosseini Chegeni Mohammad Hassan Kayedi Reza Hosseini Zakkyeh Telmadarraiy <p><strong>Multiplex-PCR differentiation of two <em>Hyalomma </em>and two <em>Haemaphysalis </em>species (Acari: Ixodidae)</strong></p> Persian Journal of Acarology Closely related species COI ITS2 Hyalomma Haemaphysalis Identification |
| title | <p><strong>Multiplex-PCR differentiation of two <em>Hyalomma </em>and two <em>Haemaphysalis </em>species (Acari: Ixodidae)</strong></p> |
| title_full | <p><strong>Multiplex-PCR differentiation of two <em>Hyalomma </em>and two <em>Haemaphysalis </em>species (Acari: Ixodidae)</strong></p> |
| title_fullStr | <p><strong>Multiplex-PCR differentiation of two <em>Hyalomma </em>and two <em>Haemaphysalis </em>species (Acari: Ixodidae)</strong></p> |
| title_full_unstemmed | <p><strong>Multiplex-PCR differentiation of two <em>Hyalomma </em>and two <em>Haemaphysalis </em>species (Acari: Ixodidae)</strong></p> |
| title_short | <p><strong>Multiplex-PCR differentiation of two <em>Hyalomma </em>and two <em>Haemaphysalis </em>species (Acari: Ixodidae)</strong></p> |
| title_sort | p strong multiplex pcr differentiation of two em hyalomma em and two em haemaphysalis em species acari ixodidae strong p |
| topic | Closely related species COI ITS2 Hyalomma Haemaphysalis Identification |
| url | https://www.biotaxa.org/pja/article/view/24392 |
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