Bisphenol B restrains rat leydig cell function via H3K27me3/H3K9me3 histone modifications
As an alternative compound of bisphenol A (BPA), bisphenol B (BPB) was widely used in plastic materials. The potential actions of BPB on the function of Leydig cells through the regulation of H3K27me3 and H3K9me3 remains unclear. Our goal was to assess how BPB influences Leydig cell function via his...
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2025-02-01
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| author | Jiayi He Huiqian Zhang Hehua Quan Qingyuan Wang Congcong Wen Yiyan Wang Yang Zhu Ren-Shan Ge Xiaoheng Li |
| author_facet | Jiayi He Huiqian Zhang Hehua Quan Qingyuan Wang Congcong Wen Yiyan Wang Yang Zhu Ren-Shan Ge Xiaoheng Li |
| author_sort | Jiayi He |
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| description | As an alternative compound of bisphenol A (BPA), bisphenol B (BPB) was widely used in plastic materials. The potential actions of BPB on the function of Leydig cells through the regulation of H3K27me3 and H3K9me3 remains unclear. Our goal was to assess how BPB influences Leydig cell function via histone modifications mediated by H3K27me3 and H3K9me3. Male 56-day-old Sprague-Dawley rats were given with 0, 50, 100, and 200 mg/kg/day of BPB by the oral administration for 14 days to study the impact of BPB on the function of Leydig cells in rats. The findings indicated that BPB significantly reduced the serum testosterone levels at the dose of 100 mg/kg and 200 mg/kg and follicle-stimulating hormone levels at the doses of 50, 100, and 200 mg/kg, while increasing estradiol levels at the dose of 200 mg/kg. BPB did not alter the numbers of CYP11A1+ Leydig cells and SOX9+ Sertoli cells, but it downregulated the expression of key genes in testosterone synthesis pathway (Lhcgr, Scarb1, Star, Cyp11a1, Cyp17a1, Hsd11b1, Hsd17b3, and Insl3) and their corresponding protein levels. Notably, BPB significantly boosted the expressions of histone methylation markers like EEF1A1, SUZ12, EED, EZH2, H3K27me3, and H3K9me3 in vivo. H3K27me3 and H3K9me3 levels were enhanced at the proximal promoters of Lhcgr, Cyp11a1, and Star through ChIP and PCR analyses. Furthermore, adult Leydig cells were extracted and cultured with BPB (0, 10, 50, 100, and 200 μM) alone or in combination with H3K27me3 antagonist GSK-J4. The results demonstrated that BPB significantly decreased testosterone output, which was counteracted by GSK-J4 to reverse BPB-mediated testosterone suppression. Additionally, BPB significantly elevated the levels of EEF1A1, EEF1A2, EED, H3K27me3, and H3K9me3 in vitro. BPB could potentially hinder the growth and function of Leydig cells by modulating H3K27me3 and H3K9me3. The findings of the study indicate the involvement of histone methylation (H3K27me3) in BPB-induced steroidogenic dysfunction, emphasizing the correlation between histone modifications and male reproductive toxicity. |
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| institution | Kabale University |
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| publishDate | 2025-02-01 |
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| spelling | doaj-art-fc58fa94764b49dd840841675cd999262025-02-07T04:46:37ZengElsevierEcotoxicology and Environmental Safety0147-65132025-02-01291117847Bisphenol B restrains rat leydig cell function via H3K27me3/H3K9me3 histone modificationsJiayi He0Huiqian Zhang1Hehua Quan2Qingyuan Wang3Congcong Wen4Yiyan Wang5Yang Zhu6Ren-Shan Ge7Xiaoheng Li8Department of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Precision Anesthesiology of Zhejiang Province, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Pediatric Anesthesiology, Ministry of Education, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Environment and Male Reproductive Medicine of Wenzhou, Wenzhou, Zhejiang 325000, ChinaDepartment of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Precision Anesthesiology of Zhejiang Province, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Pediatric Anesthesiology, Ministry of Education, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Environment and Male Reproductive Medicine of Wenzhou, Wenzhou, Zhejiang 325000, ChinaDepartment of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Precision Anesthesiology of Zhejiang Province, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Pediatric Anesthesiology, Ministry of Education, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Environment and Male Reproductive Medicine of Wenzhou, Wenzhou, Zhejiang 325000, ChinaDepartment of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Precision Anesthesiology of Zhejiang Province, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Pediatric Anesthesiology, Ministry of Education, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Environment and Male Reproductive Medicine of Wenzhou, Wenzhou, Zhejiang 325000, ChinaLaboratory Animal Centre, Wenzhou Medical University, Wenzhou, ChinaDepartment of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Precision Anesthesiology of Zhejiang Province, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Pediatric Anesthesiology, Ministry of Education, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Environment and Male Reproductive Medicine of Wenzhou, Wenzhou, Zhejiang 325000, ChinaDepartment of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Precision Anesthesiology of Zhejiang Province, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Pediatric Anesthesiology, Ministry of Education, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Environment and Male Reproductive Medicine of Wenzhou, Wenzhou, Zhejiang 325000, ChinaDepartment of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Precision Anesthesiology of Zhejiang Province, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Pediatric Anesthesiology, Ministry of Education, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Environment and Male Reproductive Medicine of Wenzhou, Wenzhou, Zhejiang 325000, China; Correspondence to: Department of Anesthesiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.Department of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Precision Anesthesiology of Zhejiang Province, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Pediatric Anesthesiology, Ministry of Education, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; Key Laboratory of Environment and Male Reproductive Medicine of Wenzhou, Wenzhou, Zhejiang 325000, China; Correspondence to: Department of Anesthesiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.As an alternative compound of bisphenol A (BPA), bisphenol B (BPB) was widely used in plastic materials. The potential actions of BPB on the function of Leydig cells through the regulation of H3K27me3 and H3K9me3 remains unclear. Our goal was to assess how BPB influences Leydig cell function via histone modifications mediated by H3K27me3 and H3K9me3. Male 56-day-old Sprague-Dawley rats were given with 0, 50, 100, and 200 mg/kg/day of BPB by the oral administration for 14 days to study the impact of BPB on the function of Leydig cells in rats. The findings indicated that BPB significantly reduced the serum testosterone levels at the dose of 100 mg/kg and 200 mg/kg and follicle-stimulating hormone levels at the doses of 50, 100, and 200 mg/kg, while increasing estradiol levels at the dose of 200 mg/kg. BPB did not alter the numbers of CYP11A1+ Leydig cells and SOX9+ Sertoli cells, but it downregulated the expression of key genes in testosterone synthesis pathway (Lhcgr, Scarb1, Star, Cyp11a1, Cyp17a1, Hsd11b1, Hsd17b3, and Insl3) and their corresponding protein levels. Notably, BPB significantly boosted the expressions of histone methylation markers like EEF1A1, SUZ12, EED, EZH2, H3K27me3, and H3K9me3 in vivo. H3K27me3 and H3K9me3 levels were enhanced at the proximal promoters of Lhcgr, Cyp11a1, and Star through ChIP and PCR analyses. Furthermore, adult Leydig cells were extracted and cultured with BPB (0, 10, 50, 100, and 200 μM) alone or in combination with H3K27me3 antagonist GSK-J4. The results demonstrated that BPB significantly decreased testosterone output, which was counteracted by GSK-J4 to reverse BPB-mediated testosterone suppression. Additionally, BPB significantly elevated the levels of EEF1A1, EEF1A2, EED, H3K27me3, and H3K9me3 in vitro. BPB could potentially hinder the growth and function of Leydig cells by modulating H3K27me3 and H3K9me3. The findings of the study indicate the involvement of histone methylation (H3K27me3) in BPB-induced steroidogenic dysfunction, emphasizing the correlation between histone modifications and male reproductive toxicity.http://www.sciencedirect.com/science/article/pii/S0147651325001836Bisphenol BLeydig cellsTestosteroneH3K27me3/H3K9me3 modificationLhcgrCyp11a1 |
| spellingShingle | Jiayi He Huiqian Zhang Hehua Quan Qingyuan Wang Congcong Wen Yiyan Wang Yang Zhu Ren-Shan Ge Xiaoheng Li Bisphenol B restrains rat leydig cell function via H3K27me3/H3K9me3 histone modifications Ecotoxicology and Environmental Safety Bisphenol B Leydig cells Testosterone H3K27me3/H3K9me3 modification Lhcgr Cyp11a1 |
| title | Bisphenol B restrains rat leydig cell function via H3K27me3/H3K9me3 histone modifications |
| title_full | Bisphenol B restrains rat leydig cell function via H3K27me3/H3K9me3 histone modifications |
| title_fullStr | Bisphenol B restrains rat leydig cell function via H3K27me3/H3K9me3 histone modifications |
| title_full_unstemmed | Bisphenol B restrains rat leydig cell function via H3K27me3/H3K9me3 histone modifications |
| title_short | Bisphenol B restrains rat leydig cell function via H3K27me3/H3K9me3 histone modifications |
| title_sort | bisphenol b restrains rat leydig cell function via h3k27me3 h3k9me3 histone modifications |
| topic | Bisphenol B Leydig cells Testosterone H3K27me3/H3K9me3 modification Lhcgr Cyp11a1 |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325001836 |
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