Identification of prognostic hub genes and functional role of BAIAP2L2 in prostate cancer progression: a transcriptomic and experimental study
BackgroundProstate cancer (PCa) is a prevalent malignancy in men, and understanding its molecular mechanisms is crucial for identifying therapeutic targets.MethodsTranscriptomic data from prostate tumors and matched healthy tissues were obtained from The Cancer Genome Atlas (TCGA). Differential expr...
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1543476/full |
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| author | Xiangyang Zhan Xiangyang Zhan Xiangyang Zhan Xiangyang Zhan Wenkai Wang Jie Lian Yichun Li Jianyi Gu Jianyi Gu Jianyi Gu Jianyi Gu Dongdong Guo Dongdong Guo Dongdong Guo Dongdong Guo Dongliang Xu Dongliang Xu Dongliang Xu Dongliang Xu Guanqun Ju Guanqun Ju Guanqun Ju Guanqun Ju |
| author_facet | Xiangyang Zhan Xiangyang Zhan Xiangyang Zhan Xiangyang Zhan Wenkai Wang Jie Lian Yichun Li Jianyi Gu Jianyi Gu Jianyi Gu Jianyi Gu Dongdong Guo Dongdong Guo Dongdong Guo Dongdong Guo Dongliang Xu Dongliang Xu Dongliang Xu Dongliang Xu Guanqun Ju Guanqun Ju Guanqun Ju Guanqun Ju |
| author_sort | Xiangyang Zhan |
| collection | DOAJ |
| description | BackgroundProstate cancer (PCa) is a prevalent malignancy in men, and understanding its molecular mechanisms is crucial for identifying therapeutic targets.MethodsTranscriptomic data from prostate tumors and matched healthy tissues were obtained from The Cancer Genome Atlas (TCGA). Differential expression analysis using the DESeq2 algorithm identified differentially expressed genes (DEGs). Cox proportional hazards regression was used to evaluate prognostic significance. Clinical validation involved comparing tumor specimens with normal tissues, focusing on BAIAP2L2, which showed significant differential expression and was further examined via immunohistochemical analysis. In vitro knockdown experiments were conducted in PC3 and DU145 cell lines to assess BAIAP2L2’s functional role through assays for migration, colony formation, and proliferation.ResultsA total of 1,449 DEGs were identified, including 775 upregulated and 674 downregulated genes. Prognostic analysis revealed 748 genes linked to clinical outcomes, with 19 hub genes identified. QPCR confirmed significant upregulation of four candidates, including BAIAP2L2, which was also elevated in malignant tissues. BAIAP2L2 knockdown significantly impaired migration, proliferation, and viability in PCa cells.ConclusionThis study highlights crucial molecular mechanisms in PCa progression, particularly the significance of BAIAP2L2 as a potential therapeutic target, warranting further investigation into additional hub genes for effective targeted strategies. |
| format | Article |
| id | doaj-art-fbff6a7180e646ed89d0839e9b583d36 |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj-art-fbff6a7180e646ed89d0839e9b583d362025-08-20T03:53:43ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-04-011610.3389/fimmu.2025.15434761543476Identification of prognostic hub genes and functional role of BAIAP2L2 in prostate cancer progression: a transcriptomic and experimental studyXiangyang Zhan0Xiangyang Zhan1Xiangyang Zhan2Xiangyang Zhan3Wenkai Wang4Jie Lian5Yichun Li6Jianyi Gu7Jianyi Gu8Jianyi Gu9Jianyi Gu10Dongdong Guo11Dongdong Guo12Dongdong Guo13Dongdong Guo14Dongliang Xu15Dongliang Xu16Dongliang Xu17Dongliang Xu18Guanqun Ju19Guanqun Ju20Guanqun Ju21Guanqun Ju22Urology Center, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaSurgical Institute of Integrative Medicine, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaSurgical Institute, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaShanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shanghai, ChinaDepartment of Oncology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People‘s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, ChinaSchool of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan, ChinaUrology Center, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaSurgical Institute of Integrative Medicine, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaSurgical Institute, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaShanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shanghai, ChinaUrology Center, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaSurgical Institute of Integrative Medicine, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaSurgical Institute, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaShanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shanghai, ChinaUrology Center, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaSurgical Institute of Integrative Medicine, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaSurgical Institute, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaShanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shanghai, ChinaUrology Center, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaSurgical Institute of Integrative Medicine, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaSurgical Institute, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaShanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shanghai, ChinaBackgroundProstate cancer (PCa) is a prevalent malignancy in men, and understanding its molecular mechanisms is crucial for identifying therapeutic targets.MethodsTranscriptomic data from prostate tumors and matched healthy tissues were obtained from The Cancer Genome Atlas (TCGA). Differential expression analysis using the DESeq2 algorithm identified differentially expressed genes (DEGs). Cox proportional hazards regression was used to evaluate prognostic significance. Clinical validation involved comparing tumor specimens with normal tissues, focusing on BAIAP2L2, which showed significant differential expression and was further examined via immunohistochemical analysis. In vitro knockdown experiments were conducted in PC3 and DU145 cell lines to assess BAIAP2L2’s functional role through assays for migration, colony formation, and proliferation.ResultsA total of 1,449 DEGs were identified, including 775 upregulated and 674 downregulated genes. Prognostic analysis revealed 748 genes linked to clinical outcomes, with 19 hub genes identified. QPCR confirmed significant upregulation of four candidates, including BAIAP2L2, which was also elevated in malignant tissues. BAIAP2L2 knockdown significantly impaired migration, proliferation, and viability in PCa cells.ConclusionThis study highlights crucial molecular mechanisms in PCa progression, particularly the significance of BAIAP2L2 as a potential therapeutic target, warranting further investigation into additional hub genes for effective targeted strategies.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1543476/fullprostate cancerBAIAP2L2transcriptomic analysisgene expressionbiomarkerstherapeutic targets |
| spellingShingle | Xiangyang Zhan Xiangyang Zhan Xiangyang Zhan Xiangyang Zhan Wenkai Wang Jie Lian Yichun Li Jianyi Gu Jianyi Gu Jianyi Gu Jianyi Gu Dongdong Guo Dongdong Guo Dongdong Guo Dongdong Guo Dongliang Xu Dongliang Xu Dongliang Xu Dongliang Xu Guanqun Ju Guanqun Ju Guanqun Ju Guanqun Ju Identification of prognostic hub genes and functional role of BAIAP2L2 in prostate cancer progression: a transcriptomic and experimental study Frontiers in Immunology prostate cancer BAIAP2L2 transcriptomic analysis gene expression biomarkers therapeutic targets |
| title | Identification of prognostic hub genes and functional role of BAIAP2L2 in prostate cancer progression: a transcriptomic and experimental study |
| title_full | Identification of prognostic hub genes and functional role of BAIAP2L2 in prostate cancer progression: a transcriptomic and experimental study |
| title_fullStr | Identification of prognostic hub genes and functional role of BAIAP2L2 in prostate cancer progression: a transcriptomic and experimental study |
| title_full_unstemmed | Identification of prognostic hub genes and functional role of BAIAP2L2 in prostate cancer progression: a transcriptomic and experimental study |
| title_short | Identification of prognostic hub genes and functional role of BAIAP2L2 in prostate cancer progression: a transcriptomic and experimental study |
| title_sort | identification of prognostic hub genes and functional role of baiap2l2 in prostate cancer progression a transcriptomic and experimental study |
| topic | prostate cancer BAIAP2L2 transcriptomic analysis gene expression biomarkers therapeutic targets |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1543476/full |
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