Site-Specific Gut Microbial Signatures in Non-Celiac Gluten Sensitivity
Gut microbiota in non-celiac gluten sensitivity (NCGS) has been poorly studied for its involvement in the disorder and site specificity. We investigated small intestinal, large intestinal and stool microbiota profiles in patients with NCGS and highly overlapping disorder irritable bowel syndrome (IB...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Gut Microbes Reports |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/29933935.2024.2438621 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850150045382344704 |
|---|---|
| author | Kunal Dixit Anam Ahmed Alka Singh Mitali Inamdar Sonal Chavan Rahul Bodkhe Wajiha Mehtab Ashish Chauhan Sunil D. Saroj Vineet Ahuja Yogesh Shouche Dhiraj Dhotre Govind Makharia |
| author_facet | Kunal Dixit Anam Ahmed Alka Singh Mitali Inamdar Sonal Chavan Rahul Bodkhe Wajiha Mehtab Ashish Chauhan Sunil D. Saroj Vineet Ahuja Yogesh Shouche Dhiraj Dhotre Govind Makharia |
| author_sort | Kunal Dixit |
| collection | DOAJ |
| description | Gut microbiota in non-celiac gluten sensitivity (NCGS) has been poorly studied for its involvement in the disorder and site specificity. We investigated small intestinal, large intestinal and stool microbiota profiles in patients with NCGS and highly overlapping disorder irritable bowel syndrome (IBS) as well as effect of gluten-free diet (GFD) on microbiota in patients with NCGS. True NCGS patients were recruited based on serological response for anti-gliadin antibodies, 6-week gluten free diet (GFD) and symptom recurrence with gluten-rechallenge. Analyses using 16S rRNA gene amplicon and shotgun sequencing revealed community differences in core microbiome and diversity measures across sample types indicating dysbiosis mainly in mucosa-associated small intestinal microbiome of NCGS patients. Genera Elusimicrobiaum, Succinivibrio, Bacillus and Alcaligenes appeared as signatures in small intestine and stool in NCGS patients. Presence of differential taxa co-occurring at sampling sites, enabled recognition of site-specific microbial signatures. GFD led to a shift in mucosa-associated small intestinal core microbiome. Metagenome analysis revealed subtle differences in pathways for amino acid biosynthesis including L-ornithine. Mucosa-associated small intestine microbial structure was quite distinct in patients with NCGS in comparison to that with IBS. |
| format | Article |
| id | doaj-art-fbf7f90c184b4c8ea2af20956482ec7f |
| institution | OA Journals |
| issn | 2993-3935 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes Reports |
| spelling | doaj-art-fbf7f90c184b4c8ea2af20956482ec7f2025-08-20T02:26:41ZengTaylor & Francis GroupGut Microbes Reports2993-39352024-12-011110.1080/29933935.2024.2438621Site-Specific Gut Microbial Signatures in Non-Celiac Gluten SensitivityKunal Dixit0Anam Ahmed1Alka Singh2Mitali Inamdar3Sonal Chavan4Rahul Bodkhe5Wajiha Mehtab6Ashish Chauhan7Sunil D. Saroj8Vineet Ahuja9Yogesh Shouche10Dhiraj Dhotre11Govind Makharia12Symbiosis School of Biological Sciences, Symbiosis International (Deemed University), Pune, IndiaDepartment of Gastroenterology and Human Nutrition, All India Institute of Medical Sciences, New Delhi, IndiaDepartment of Gastroenterology and Human Nutrition, All India Institute of Medical Sciences, New Delhi, IndiaNational Centre for Cell Science, Pune, IndiaNational Centre for Cell Science, Pune, IndiaDepartment of Integrative Biology, University of California, Berkeley, USADepartment of Home Science, University of Delhi, New Delhi, IndiaDepartment of Gastroenterology and Human Nutrition, All India Institute of Medical Sciences, New Delhi, IndiaSymbiosis School of Biological Sciences, Symbiosis International (Deemed University), Pune, IndiaDepartment of Gastroenterology and Human Nutrition, All India Institute of Medical Sciences, New Delhi, IndiaNational Centre for Cell Science, Pune, IndiaNational Centre for Cell Science, Pune, IndiaDepartment of Gastroenterology and Human Nutrition, All India Institute of Medical Sciences, New Delhi, IndiaGut microbiota in non-celiac gluten sensitivity (NCGS) has been poorly studied for its involvement in the disorder and site specificity. We investigated small intestinal, large intestinal and stool microbiota profiles in patients with NCGS and highly overlapping disorder irritable bowel syndrome (IBS) as well as effect of gluten-free diet (GFD) on microbiota in patients with NCGS. True NCGS patients were recruited based on serological response for anti-gliadin antibodies, 6-week gluten free diet (GFD) and symptom recurrence with gluten-rechallenge. Analyses using 16S rRNA gene amplicon and shotgun sequencing revealed community differences in core microbiome and diversity measures across sample types indicating dysbiosis mainly in mucosa-associated small intestinal microbiome of NCGS patients. Genera Elusimicrobiaum, Succinivibrio, Bacillus and Alcaligenes appeared as signatures in small intestine and stool in NCGS patients. Presence of differential taxa co-occurring at sampling sites, enabled recognition of site-specific microbial signatures. GFD led to a shift in mucosa-associated small intestinal core microbiome. Metagenome analysis revealed subtle differences in pathways for amino acid biosynthesis including L-ornithine. Mucosa-associated small intestine microbial structure was quite distinct in patients with NCGS in comparison to that with IBS.https://www.tandfonline.com/doi/10.1080/29933935.2024.2438621Gluten-free dietirritable bowel syndrome16S rRNA amplicon libraryshotgun metagenomesmall intestinehuman microbiome |
| spellingShingle | Kunal Dixit Anam Ahmed Alka Singh Mitali Inamdar Sonal Chavan Rahul Bodkhe Wajiha Mehtab Ashish Chauhan Sunil D. Saroj Vineet Ahuja Yogesh Shouche Dhiraj Dhotre Govind Makharia Site-Specific Gut Microbial Signatures in Non-Celiac Gluten Sensitivity Gut Microbes Reports Gluten-free diet irritable bowel syndrome 16S rRNA amplicon library shotgun metagenome small intestine human microbiome |
| title | Site-Specific Gut Microbial Signatures in Non-Celiac Gluten Sensitivity |
| title_full | Site-Specific Gut Microbial Signatures in Non-Celiac Gluten Sensitivity |
| title_fullStr | Site-Specific Gut Microbial Signatures in Non-Celiac Gluten Sensitivity |
| title_full_unstemmed | Site-Specific Gut Microbial Signatures in Non-Celiac Gluten Sensitivity |
| title_short | Site-Specific Gut Microbial Signatures in Non-Celiac Gluten Sensitivity |
| title_sort | site specific gut microbial signatures in non celiac gluten sensitivity |
| topic | Gluten-free diet irritable bowel syndrome 16S rRNA amplicon library shotgun metagenome small intestine human microbiome |
| url | https://www.tandfonline.com/doi/10.1080/29933935.2024.2438621 |
| work_keys_str_mv | AT kunaldixit sitespecificgutmicrobialsignaturesinnonceliacglutensensitivity AT anamahmed sitespecificgutmicrobialsignaturesinnonceliacglutensensitivity AT alkasingh sitespecificgutmicrobialsignaturesinnonceliacglutensensitivity AT mitaliinamdar sitespecificgutmicrobialsignaturesinnonceliacglutensensitivity AT sonalchavan sitespecificgutmicrobialsignaturesinnonceliacglutensensitivity AT rahulbodkhe sitespecificgutmicrobialsignaturesinnonceliacglutensensitivity AT wajihamehtab sitespecificgutmicrobialsignaturesinnonceliacglutensensitivity AT ashishchauhan sitespecificgutmicrobialsignaturesinnonceliacglutensensitivity AT sunildsaroj sitespecificgutmicrobialsignaturesinnonceliacglutensensitivity AT vineetahuja sitespecificgutmicrobialsignaturesinnonceliacglutensensitivity AT yogeshshouche sitespecificgutmicrobialsignaturesinnonceliacglutensensitivity AT dhirajdhotre sitespecificgutmicrobialsignaturesinnonceliacglutensensitivity AT govindmakharia sitespecificgutmicrobialsignaturesinnonceliacglutensensitivity |