Isolation and characterization of pathogenic Vibrio on tiger grouper Epinephelus fuscoguttatus
This study was aimed to obtain pathogenic bacterial isolate causing vibriosis disease. Isolation of Vibrio was conducted from maribound tiger grouper collected from floating net cage in Barru Regency using TCBS medium. Ability to cause vibriosis was confirmed by pathogenicity test performed by mean...
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Asosiasi Sains Akuakultur Indonesia
2013-11-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/jai/article/view/7195 |
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| author | . Ilmiah . Sukenda . Widanarni Enang Harris |
| author_facet | . Ilmiah . Sukenda . Widanarni Enang Harris |
| author_sort | . Ilmiah |
| collection | DOAJ |
| description |
This study was aimed to obtain pathogenic bacterial isolate causing vibriosis disease. Isolation of Vibrio was conducted from maribound tiger grouper collected from floating net cage in Barru Regency using TCBS medium. Ability to cause vibriosis was confirmed by pathogenicity test performed by mean injecting the tiger grouper juveniles with bacterial suspension at concentration of 106 CFU/fish and mortality of fish during seven days observation then was noted. Then, the Vibrio pathogenic isolate was characterized and identified based on morphology, growth, and biochemical features. Moreover, the most pathogenic isolate was identified by molecular analysis of 16S-rRNA gene sequences. The results showed that three potential isolates caused Vibriosis disease in tiger grouper culture. The isolates tested were biochemically identified as Vibrio metschnikovii, V. parahaemolyticus, and V. mimicus. The most virulent among isolates was V. parahaemolyticus.
Keywords: isolation, characterization, pathogenic, vibriosis, tiger grouper
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| format | Article |
| id | doaj-art-fbec33690c7a4f41b52b590ffdd248a5 |
| institution | Kabale University |
| issn | 1412-5269 2354-6700 |
| language | English |
| publishDate | 2013-11-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
| record_format | Article |
| series | Jurnal Akuakultur Indonesia |
| spelling | doaj-art-fbec33690c7a4f41b52b590ffdd248a52025-01-24T18:00:43ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002013-11-0111110.19027/jai.11.28-376263Isolation and characterization of pathogenic Vibrio on tiger grouper Epinephelus fuscoguttatus. Ilmiah0. Sukenda1. Widanarni2Enang Harris3Jurusan Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Muslim Indonesia Makassar Jl. Kakatua No. 27 Makassar 90121Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan,Institut Pertanian BogorDepartemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan,Institut Pertanian BogorDepartemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan,Institut Pertanian Bogor This study was aimed to obtain pathogenic bacterial isolate causing vibriosis disease. Isolation of Vibrio was conducted from maribound tiger grouper collected from floating net cage in Barru Regency using TCBS medium. Ability to cause vibriosis was confirmed by pathogenicity test performed by mean injecting the tiger grouper juveniles with bacterial suspension at concentration of 106 CFU/fish and mortality of fish during seven days observation then was noted. Then, the Vibrio pathogenic isolate was characterized and identified based on morphology, growth, and biochemical features. Moreover, the most pathogenic isolate was identified by molecular analysis of 16S-rRNA gene sequences. The results showed that three potential isolates caused Vibriosis disease in tiger grouper culture. The isolates tested were biochemically identified as Vibrio metschnikovii, V. parahaemolyticus, and V. mimicus. The most virulent among isolates was V. parahaemolyticus. Keywords: isolation, characterization, pathogenic, vibriosis, tiger grouper https://journal.ipb.ac.id/index.php/jai/article/view/7195 |
| spellingShingle | . Ilmiah . Sukenda . Widanarni Enang Harris Isolation and characterization of pathogenic Vibrio on tiger grouper Epinephelus fuscoguttatus Jurnal Akuakultur Indonesia |
| title | Isolation and characterization of pathogenic Vibrio on tiger grouper Epinephelus fuscoguttatus |
| title_full | Isolation and characterization of pathogenic Vibrio on tiger grouper Epinephelus fuscoguttatus |
| title_fullStr | Isolation and characterization of pathogenic Vibrio on tiger grouper Epinephelus fuscoguttatus |
| title_full_unstemmed | Isolation and characterization of pathogenic Vibrio on tiger grouper Epinephelus fuscoguttatus |
| title_short | Isolation and characterization of pathogenic Vibrio on tiger grouper Epinephelus fuscoguttatus |
| title_sort | isolation and characterization of pathogenic vibrio on tiger grouper epinephelus fuscoguttatus |
| url | https://journal.ipb.ac.id/index.php/jai/article/view/7195 |
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