Exploration of Mangrove Endophytes as Novel Sources of Tannase Producing Fungi
Tannase, a highly adaptive biocatalyst, plays a pivotal role in diverse bioconversion reactions in nature. This enzyme exhibits numerous applications across various industrial sectors, including food, pharmaceuticals, chemicals, and beverages. This study aimed to screen and characterize fungal endop...
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MDPI AG
2025-05-01
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| author | Vinodkumar Kushwaha Jitendra R. Patil Ganesh Chandrakant Nikalje Lal Sahab Yadav |
| author_facet | Vinodkumar Kushwaha Jitendra R. Patil Ganesh Chandrakant Nikalje Lal Sahab Yadav |
| author_sort | Vinodkumar Kushwaha |
| collection | DOAJ |
| description | Tannase, a highly adaptive biocatalyst, plays a pivotal role in diverse bioconversion reactions in nature. This enzyme exhibits numerous applications across various industrial sectors, including food, pharmaceuticals, chemicals, and beverages. This study aimed to screen and characterize fungal endophytes isolated from mangrove plants for their enzyme tannase-producing ability. Eighty-five filamentous endophytic fungi were isolated from different mangrove samples and subsequently identified. These fungal strains were initially screened using the tannic acid agar plate method. Out of the screened strains, 13 fungal isolates demonstrated tannase production ability. The quantitative estimation of extracellular tannase was performed using the submerged fermentation technique. Among the studied endophytes, eight isolates, namely LV_084 (21.21 IU/mL), LV_074 (15.41 IU/mL), LV_078 (6.98 IU/mL), LV_038 (6.97 IU/mL), LV_077 (6.32 IU/mL), LV_016 and LV_066 (6.37 IU/mL), and LV_060 (6.18 IU/mL) exhibited excellent tannase activity. Among these isolates, LV_084 <i>Phyllosticta capitalensis</i> and LV_074 <i>Aspergillus chevalieri</i> showed the highest enzyme-producing ability. These isolates were authenticated using ITS rDNA sequencing, followed by BLAST search and phylogenetic analysis. Furthermore, the physical and chemical conditions for the maximum enzyme production were optimized. This is the first report of enzyme tannase production by <i>Phyllosticta capitalensis</i> and <i>Aspergillus chevalieri</i>. |
| format | Article |
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| language | English |
| publishDate | 2025-05-01 |
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| series | Journal of Fungi |
| spelling | doaj-art-fbccad897c0748178f1d3c5e6a6d66cc2025-08-20T01:56:16ZengMDPI AGJournal of Fungi2309-608X2025-05-0111536610.3390/jof11050366Exploration of Mangrove Endophytes as Novel Sources of Tannase Producing FungiVinodkumar Kushwaha0Jitendra R. Patil1Ganesh Chandrakant Nikalje2Lal Sahab Yadav3Post Graduate Research Laboratory, Department of Botany, Smt. Chandibai Himathmal Mansukhani College of Arts, Science & Commerce, Ulhasnagar 421003, Thane, MS, IndiaPost Graduate Research Laboratory, Department of Botany, Smt. Chandibai Himathmal Mansukhani College of Arts, Science & Commerce, Ulhasnagar 421003, Thane, MS, IndiaDepartment of Botany, Seva Sadan’s Ramchand Kimatram Talreja College of Arts, Science & Commerce, Ulhasnagar 421003, Thane, MS, IndiaPost Graduate Research Laboratory, Department of Botany, Smt. Chandibai Himathmal Mansukhani College of Arts, Science & Commerce, Ulhasnagar 421003, Thane, MS, IndiaTannase, a highly adaptive biocatalyst, plays a pivotal role in diverse bioconversion reactions in nature. This enzyme exhibits numerous applications across various industrial sectors, including food, pharmaceuticals, chemicals, and beverages. This study aimed to screen and characterize fungal endophytes isolated from mangrove plants for their enzyme tannase-producing ability. Eighty-five filamentous endophytic fungi were isolated from different mangrove samples and subsequently identified. These fungal strains were initially screened using the tannic acid agar plate method. Out of the screened strains, 13 fungal isolates demonstrated tannase production ability. The quantitative estimation of extracellular tannase was performed using the submerged fermentation technique. Among the studied endophytes, eight isolates, namely LV_084 (21.21 IU/mL), LV_074 (15.41 IU/mL), LV_078 (6.98 IU/mL), LV_038 (6.97 IU/mL), LV_077 (6.32 IU/mL), LV_016 and LV_066 (6.37 IU/mL), and LV_060 (6.18 IU/mL) exhibited excellent tannase activity. Among these isolates, LV_084 <i>Phyllosticta capitalensis</i> and LV_074 <i>Aspergillus chevalieri</i> showed the highest enzyme-producing ability. These isolates were authenticated using ITS rDNA sequencing, followed by BLAST search and phylogenetic analysis. Furthermore, the physical and chemical conditions for the maximum enzyme production were optimized. This is the first report of enzyme tannase production by <i>Phyllosticta capitalensis</i> and <i>Aspergillus chevalieri</i>.https://www.mdpi.com/2309-608X/11/5/366mangroveendophytic fungiscreeningtannase |
| spellingShingle | Vinodkumar Kushwaha Jitendra R. Patil Ganesh Chandrakant Nikalje Lal Sahab Yadav Exploration of Mangrove Endophytes as Novel Sources of Tannase Producing Fungi Journal of Fungi mangrove endophytic fungi screening tannase |
| title | Exploration of Mangrove Endophytes as Novel Sources of Tannase Producing Fungi |
| title_full | Exploration of Mangrove Endophytes as Novel Sources of Tannase Producing Fungi |
| title_fullStr | Exploration of Mangrove Endophytes as Novel Sources of Tannase Producing Fungi |
| title_full_unstemmed | Exploration of Mangrove Endophytes as Novel Sources of Tannase Producing Fungi |
| title_short | Exploration of Mangrove Endophytes as Novel Sources of Tannase Producing Fungi |
| title_sort | exploration of mangrove endophytes as novel sources of tannase producing fungi |
| topic | mangrove endophytic fungi screening tannase |
| url | https://www.mdpi.com/2309-608X/11/5/366 |
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