Comparing Multiplex PCR and Rapid Urease Test in the Detection of H. pylori in Patients on Proton Pump Inhibitors
Background. This study was conducted to assess the diagnostic value of a multiplex PCR assay to detect H. pylori infection and to further evaluate the negative results from the CLOtest on patients with and without PPI treatment. Methods. This study is a retrospective cohort that included 457 patient...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2012/898276 |
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| author | Thomas Chen Xiangwen Meng H. Zhang Rebecca W. Tsang Tat-Kin Tsang |
| author_facet | Thomas Chen Xiangwen Meng H. Zhang Rebecca W. Tsang Tat-Kin Tsang |
| author_sort | Thomas Chen |
| collection | DOAJ |
| description | Background. This study was conducted to assess the diagnostic value of a multiplex PCR assay to detect H. pylori infection and to further evaluate the negative results from the CLOtest on patients with and without PPI treatment. Methods. This study is a retrospective cohort that included 457 patients with symptoms of dyspepsia, who underwent upper endoscopy at Evanston and Glenbrook Northshore Hospital from June 2003 to October 2007. A total of 556 samples were reported with some patients having more than one test over the time period. The CLOtest was performed first on the gastric specimen and from that specimen, the DNA was isolated and the one-step multiplex PCR was performed. Results. By M-PCR testing, H. pylori was detected in 143 (52.2%) of 274 cases in the control group and 130 (46.1%) of 282 cases in patients on PPI treatment (P=0.1746). The CLOtest detected the presence of H. pylori in 4 (1.4%) of 282 cases from the same group receiving PPI treatment and 29 (10.6%) of 274 cases from the group not taking a PPI (P≤0.0001). Conclusion. Our PCR is sensitive enough to detect the presence of H. pylori despite being on PPI treatment. |
| format | Article |
| id | doaj-art-fbca14609adf4f6bb5c05ec7eae3728b |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
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| series | Gastroenterology Research and Practice |
| spelling | doaj-art-fbca14609adf4f6bb5c05ec7eae3728b2025-08-20T03:25:34ZengWileyGastroenterology Research and Practice1687-61211687-630X2012-01-01201210.1155/2012/898276898276Comparing Multiplex PCR and Rapid Urease Test in the Detection of H. pylori in Patients on Proton Pump InhibitorsThomas Chen0Xiangwen Meng1H. Zhang2Rebecca W. Tsang3Tat-Kin Tsang4College of Medicine, University of Illinois at Chicago, 1853 West Polk Street, Chicago, IL 60612, USATZAM Diagnostics LLC, 1824 Wilmette Avenue, Wilmette, IL 60091, USATZAM Diagnostics LLC, 1824 Wilmette Avenue, Wilmette, IL 60091, USADepartment of Medicine, Loyola University Hospital, Maywood, IL 60153, USADepartment of Gastroenterology, NorthShore University Health System, University of Chicago, Glenview, IL 60026, USABackground. This study was conducted to assess the diagnostic value of a multiplex PCR assay to detect H. pylori infection and to further evaluate the negative results from the CLOtest on patients with and without PPI treatment. Methods. This study is a retrospective cohort that included 457 patients with symptoms of dyspepsia, who underwent upper endoscopy at Evanston and Glenbrook Northshore Hospital from June 2003 to October 2007. A total of 556 samples were reported with some patients having more than one test over the time period. The CLOtest was performed first on the gastric specimen and from that specimen, the DNA was isolated and the one-step multiplex PCR was performed. Results. By M-PCR testing, H. pylori was detected in 143 (52.2%) of 274 cases in the control group and 130 (46.1%) of 282 cases in patients on PPI treatment (P=0.1746). The CLOtest detected the presence of H. pylori in 4 (1.4%) of 282 cases from the same group receiving PPI treatment and 29 (10.6%) of 274 cases from the group not taking a PPI (P≤0.0001). Conclusion. Our PCR is sensitive enough to detect the presence of H. pylori despite being on PPI treatment.http://dx.doi.org/10.1155/2012/898276 |
| spellingShingle | Thomas Chen Xiangwen Meng H. Zhang Rebecca W. Tsang Tat-Kin Tsang Comparing Multiplex PCR and Rapid Urease Test in the Detection of H. pylori in Patients on Proton Pump Inhibitors Gastroenterology Research and Practice |
| title | Comparing Multiplex PCR and Rapid Urease Test in the Detection of H. pylori in Patients on Proton Pump Inhibitors |
| title_full | Comparing Multiplex PCR and Rapid Urease Test in the Detection of H. pylori in Patients on Proton Pump Inhibitors |
| title_fullStr | Comparing Multiplex PCR and Rapid Urease Test in the Detection of H. pylori in Patients on Proton Pump Inhibitors |
| title_full_unstemmed | Comparing Multiplex PCR and Rapid Urease Test in the Detection of H. pylori in Patients on Proton Pump Inhibitors |
| title_short | Comparing Multiplex PCR and Rapid Urease Test in the Detection of H. pylori in Patients on Proton Pump Inhibitors |
| title_sort | comparing multiplex pcr and rapid urease test in the detection of h pylori in patients on proton pump inhibitors |
| url | http://dx.doi.org/10.1155/2012/898276 |
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