Effect of BMP-2 Delivery Mode on Osteogenic Differentiation of Stem Cells
Differentiation of stem cells is an important strategy for regeneration of defective tissue in stem cell therapy. Bone morphogenetic protein-2 (BMP-2) is a well-known osteogenic differentiation factor that stimulates stem cell signaling pathways by activating transmembrane type I and type II recepto...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/7859184 |
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| author | Taekhee Jung John Hwan Lee Soonjung Park Yong-Jin Kim Joseph Seo Hye-Eun Shim Ki-Suk Kim Hyon-Seok Jang Hyung-Min Chung Seong-Geun Oh Sung-Hwan Moon Sun-Woong Kang |
| author_facet | Taekhee Jung John Hwan Lee Soonjung Park Yong-Jin Kim Joseph Seo Hye-Eun Shim Ki-Suk Kim Hyon-Seok Jang Hyung-Min Chung Seong-Geun Oh Sung-Hwan Moon Sun-Woong Kang |
| author_sort | Taekhee Jung |
| collection | DOAJ |
| description | Differentiation of stem cells is an important strategy for regeneration of defective tissue in stem cell therapy. Bone morphogenetic protein-2 (BMP-2) is a well-known osteogenic differentiation factor that stimulates stem cell signaling pathways by activating transmembrane type I and type II receptors. However, BMPs have a very short half-life and may rapidly lose their bioactivity. Thus, a BMP delivery system is required to take advantage of an osteoinductive effect for osteogenic differentiation. Previously, BMP delivery has been designed and evaluated for osteogenic differentiation, focusing on carriers and sustained release system for delivery of BMPs. The effect of the delivery mode in cell culture plate on osteogenic differentiation potential was not evaluated. Herein, to investigate the effect of delivery mode on osteogenic differentiation of BM-MSCs in this study, we fabricated bottom-up release and top-down release systems for culture plate delivery of BMP-2. And also, we selected Arg-Gly-Asp- (RGD-) conjugated alginate hydrogel for BMP-2 delivery because alginate is able to release BMP-2 in a sustained manner and it is a biocompatible material. After 7 days of culture, the bottom-up release system in culture plate significantly stimulated alkaline phosphate activity of human bone marrow-mesenchymal stem cells. The present study highlights the potential value of the tool in stem cell therapy. |
| format | Article |
| id | doaj-art-fbbd4106d91e41278eb45f3f959c7235 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-fbbd4106d91e41278eb45f3f959c72352025-02-03T05:48:02ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/78591847859184Effect of BMP-2 Delivery Mode on Osteogenic Differentiation of Stem CellsTaekhee Jung0John Hwan Lee1Soonjung Park2Yong-Jin Kim3Joseph Seo4Hye-Eun Shim5Ki-Suk Kim6Hyon-Seok Jang7Hyung-Min Chung8Seong-Geun Oh9Sung-Hwan Moon10Sun-Woong Kang11Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, Republic of KoreaDepartment of Chemical Engineering, Hanyang University, Seoul, Republic of KoreaDepartment of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, Republic of KoreaAmorePacific Corp./R&D Center, Yongin-si, Gyeonggi-do, Republic of KoreaDepartment of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, Republic of KoreaPredictive Model Research Center, Korea Institute of Toxicology, Daejeon, Republic of KoreaPredictive Model Research Center, Korea Institute of Toxicology, Daejeon, Republic of KoreaDepartment of Dentistry, Korea University, Ansan Hospital, Ansan, Republic of KoreaDepartment of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, Republic of KoreaDepartment of Chemical Engineering, Hanyang University, Seoul, Republic of KoreaDepartment of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, Republic of KoreaPredictive Model Research Center, Korea Institute of Toxicology, Daejeon, Republic of KoreaDifferentiation of stem cells is an important strategy for regeneration of defective tissue in stem cell therapy. Bone morphogenetic protein-2 (BMP-2) is a well-known osteogenic differentiation factor that stimulates stem cell signaling pathways by activating transmembrane type I and type II receptors. However, BMPs have a very short half-life and may rapidly lose their bioactivity. Thus, a BMP delivery system is required to take advantage of an osteoinductive effect for osteogenic differentiation. Previously, BMP delivery has been designed and evaluated for osteogenic differentiation, focusing on carriers and sustained release system for delivery of BMPs. The effect of the delivery mode in cell culture plate on osteogenic differentiation potential was not evaluated. Herein, to investigate the effect of delivery mode on osteogenic differentiation of BM-MSCs in this study, we fabricated bottom-up release and top-down release systems for culture plate delivery of BMP-2. And also, we selected Arg-Gly-Asp- (RGD-) conjugated alginate hydrogel for BMP-2 delivery because alginate is able to release BMP-2 in a sustained manner and it is a biocompatible material. After 7 days of culture, the bottom-up release system in culture plate significantly stimulated alkaline phosphate activity of human bone marrow-mesenchymal stem cells. The present study highlights the potential value of the tool in stem cell therapy.http://dx.doi.org/10.1155/2017/7859184 |
| spellingShingle | Taekhee Jung John Hwan Lee Soonjung Park Yong-Jin Kim Joseph Seo Hye-Eun Shim Ki-Suk Kim Hyon-Seok Jang Hyung-Min Chung Seong-Geun Oh Sung-Hwan Moon Sun-Woong Kang Effect of BMP-2 Delivery Mode on Osteogenic Differentiation of Stem Cells Stem Cells International |
| title | Effect of BMP-2 Delivery Mode on Osteogenic Differentiation of Stem Cells |
| title_full | Effect of BMP-2 Delivery Mode on Osteogenic Differentiation of Stem Cells |
| title_fullStr | Effect of BMP-2 Delivery Mode on Osteogenic Differentiation of Stem Cells |
| title_full_unstemmed | Effect of BMP-2 Delivery Mode on Osteogenic Differentiation of Stem Cells |
| title_short | Effect of BMP-2 Delivery Mode on Osteogenic Differentiation of Stem Cells |
| title_sort | effect of bmp 2 delivery mode on osteogenic differentiation of stem cells |
| url | http://dx.doi.org/10.1155/2017/7859184 |
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