Antibacterial activity of Ilex paraguariensis (Yerba Mate) against Gram-positive and Gram-negative bacteria
Introduction: The stems and leaves of Ilex paraguariensis are popularly used for tea infusions in South America and the Middle East. The health benefits have been previously studied, revealing anti-mutagenic, anti-oxidant, hepatoprotective, hypocholesteremic and glycemic improvement. Limited resear...
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The Journal of Infection in Developing Countries
2018-09-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/10380 |
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| author | Tania Noureddine Ziad El Husseini Ali Nehme Roula Abdel Massih |
| author_facet | Tania Noureddine Ziad El Husseini Ali Nehme Roula Abdel Massih |
| author_sort | Tania Noureddine |
| collection | DOAJ |
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Introduction: The stems and leaves of Ilex paraguariensis are popularly used for tea infusions in South America and the Middle East. The health benefits have been previously studied, revealing anti-mutagenic, anti-oxidant, hepatoprotective, hypocholesteremic and glycemic improvement. Limited research was performed on the antibacterial activity of the aqueous extract of Yerba Mate on standard and clinical isolates of Gram-positive and Gram-negative bacteria.
Methodology: Commercial Ilex paraguariensis stems and leaves were ground and extracted with sterile deionized water at 70°C. Four ATCC bacterial strains and twenty-five bacterial clinical strains were used for testing. To obtain the minimal inhibitory concentration (MIC), the Yerba Mate aqueous solution was serially diluted according to the microdilution method. For the minimal bactericidal concentration (MBC), the tubes with clear broth were sub-cultured. To identify the types of ESBLs present in the clinical isolates, a multiplex PCR was performed.
Results: An antibacterial activity was observed against most of tested strains, with a greater activity against Gram-positive bacteria. MIC and MBC values ranged between 0.468 mg/mL and 15 mg/mL of aqueous extract of Yerba Mate.
Conclusion: The aqueous extract of the stems and leaves of Ilex paraguariensis extracted at 70°C showed a significant antibacterial activity. There was no correlation found between the different molecular resistance profiles and the antibacterial activity range.
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| format | Article |
| id | doaj-art-fbb87df3fcdb4161a1b07b2f67938999 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2018-09-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-fbb87df3fcdb4161a1b07b2f679389992025-08-20T02:57:44ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802018-09-01120910.3855/jidc.10380Antibacterial activity of Ilex paraguariensis (Yerba Mate) against Gram-positive and Gram-negative bacteriaTania Noureddine0Ziad El Husseini1Ali Nehme2Roula Abdel Massih3University of Balamand, El-Koura, LebanonUniversity of Balamand, El-Koura, LebanonUniversity of Balamand, El-Koura, LebanonUniversity of Balamand, El-Koura, Lebanon Introduction: The stems and leaves of Ilex paraguariensis are popularly used for tea infusions in South America and the Middle East. The health benefits have been previously studied, revealing anti-mutagenic, anti-oxidant, hepatoprotective, hypocholesteremic and glycemic improvement. Limited research was performed on the antibacterial activity of the aqueous extract of Yerba Mate on standard and clinical isolates of Gram-positive and Gram-negative bacteria. Methodology: Commercial Ilex paraguariensis stems and leaves were ground and extracted with sterile deionized water at 70°C. Four ATCC bacterial strains and twenty-five bacterial clinical strains were used for testing. To obtain the minimal inhibitory concentration (MIC), the Yerba Mate aqueous solution was serially diluted according to the microdilution method. For the minimal bactericidal concentration (MBC), the tubes with clear broth were sub-cultured. To identify the types of ESBLs present in the clinical isolates, a multiplex PCR was performed. Results: An antibacterial activity was observed against most of tested strains, with a greater activity against Gram-positive bacteria. MIC and MBC values ranged between 0.468 mg/mL and 15 mg/mL of aqueous extract of Yerba Mate. Conclusion: The aqueous extract of the stems and leaves of Ilex paraguariensis extracted at 70°C showed a significant antibacterial activity. There was no correlation found between the different molecular resistance profiles and the antibacterial activity range. https://jidc.org/index.php/journal/article/view/10380Yerba MateantibacterialMIC/MBC |
| spellingShingle | Tania Noureddine Ziad El Husseini Ali Nehme Roula Abdel Massih Antibacterial activity of Ilex paraguariensis (Yerba Mate) against Gram-positive and Gram-negative bacteria Journal of Infection in Developing Countries Yerba Mate antibacterial MIC/MBC |
| title | Antibacterial activity of Ilex paraguariensis (Yerba Mate) against Gram-positive and Gram-negative bacteria |
| title_full | Antibacterial activity of Ilex paraguariensis (Yerba Mate) against Gram-positive and Gram-negative bacteria |
| title_fullStr | Antibacterial activity of Ilex paraguariensis (Yerba Mate) against Gram-positive and Gram-negative bacteria |
| title_full_unstemmed | Antibacterial activity of Ilex paraguariensis (Yerba Mate) against Gram-positive and Gram-negative bacteria |
| title_short | Antibacterial activity of Ilex paraguariensis (Yerba Mate) against Gram-positive and Gram-negative bacteria |
| title_sort | antibacterial activity of ilex paraguariensis yerba mate against gram positive and gram negative bacteria |
| topic | Yerba Mate antibacterial MIC/MBC |
| url | https://jidc.org/index.php/journal/article/view/10380 |
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