Antimicrobial Effect of Platelet-Rich Plasma against Porphyromonas gingivalis
Aim. The aim of our study was to evaluate whether there was a difference in antimicrobial effect between the PRP of healthy volunteers and that of patients with chronic periodontitis against P. gingivalis, which was fresh cultured from subgingival plaque. Methods. Subgingival plaque of patients with...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2019/7329103 |
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| author | Thuy Anh Vu Pham Thao Thi Phuong Tran Ngan Thi My Luong |
| author_facet | Thuy Anh Vu Pham Thao Thi Phuong Tran Ngan Thi My Luong |
| author_sort | Thuy Anh Vu Pham |
| collection | DOAJ |
| description | Aim. The aim of our study was to evaluate whether there was a difference in antimicrobial effect between the PRP of healthy volunteers and that of patients with chronic periodontitis against P. gingivalis, which was fresh cultured from subgingival plaque. Methods. Subgingival plaque of patients with moderate and severe chronic periodontitis was collected to isolate P. gingivalis. The PRP of four individuals with healthy periodontium and four patients with moderate and severe periodontitis were collected with a specific kit using a two-centrifuge procedure, and then, the antibacterial properties against P. gingivalis were tested, through their minimum inhibitory concentration (MIC), adhesion resistance assay, and biofilm susceptibility assay. Results. P. gingivalis was successfully isolated from the subgingival plaque of the 21st patient. The round, smooth, and black colony appeared in the agar disk after 7–10 days of incubation under anaerobic conditions. Bacterial identification was performed by MALDI-TOF and confirmed by PCR. All PRP samples tested showed the ability to inhibit P. gingivalis growth. The MIC value (expressed as fraction of PRP) was 1/2, and PRP prevented P. gingivalis attachment on the disk surface. However, PRP did not have a strong effect on the suppression of P. gingivalis biofilm. Conclusion. PRP of individuals with healthy periodontium and chronic periodontitis patients showed antibacterial properties against P. gingivalis. This material can become an adjunct to periodontal treatment. |
| format | Article |
| id | doaj-art-fba26539023f464aa334b91d14b3c595 |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-fba26539023f464aa334b91d14b3c5952025-02-03T01:21:35ZengWileyInternational Journal of Dentistry1687-87281687-87362019-01-01201910.1155/2019/73291037329103Antimicrobial Effect of Platelet-Rich Plasma against Porphyromonas gingivalisThuy Anh Vu Pham0Thao Thi Phuong Tran1Ngan Thi My Luong2Department of Periodontology, Faculty of Odonto-Stomatology, University of Medicine and Pharmacy at Ho Chi Minh City, 652 Nguyen Trai Str., District 5, Ho Chi Minh City, VietnamFaculty of Odonto-Stomatology, Hong Bang International University, 120 Hoa Binh Str., District Tan Phu, Ho Chi Minh City, VietnamDepartment of Plant Biotechnology and Biotransformation, Faculty of Biology, University of Science, Vietnam National University Ho Chi Minh City, 227 Nguyen Van Cu Street, District 5, Ho Chi Minh City, VietnamAim. The aim of our study was to evaluate whether there was a difference in antimicrobial effect between the PRP of healthy volunteers and that of patients with chronic periodontitis against P. gingivalis, which was fresh cultured from subgingival plaque. Methods. Subgingival plaque of patients with moderate and severe chronic periodontitis was collected to isolate P. gingivalis. The PRP of four individuals with healthy periodontium and four patients with moderate and severe periodontitis were collected with a specific kit using a two-centrifuge procedure, and then, the antibacterial properties against P. gingivalis were tested, through their minimum inhibitory concentration (MIC), adhesion resistance assay, and biofilm susceptibility assay. Results. P. gingivalis was successfully isolated from the subgingival plaque of the 21st patient. The round, smooth, and black colony appeared in the agar disk after 7–10 days of incubation under anaerobic conditions. Bacterial identification was performed by MALDI-TOF and confirmed by PCR. All PRP samples tested showed the ability to inhibit P. gingivalis growth. The MIC value (expressed as fraction of PRP) was 1/2, and PRP prevented P. gingivalis attachment on the disk surface. However, PRP did not have a strong effect on the suppression of P. gingivalis biofilm. Conclusion. PRP of individuals with healthy periodontium and chronic periodontitis patients showed antibacterial properties against P. gingivalis. This material can become an adjunct to periodontal treatment.http://dx.doi.org/10.1155/2019/7329103 |
| spellingShingle | Thuy Anh Vu Pham Thao Thi Phuong Tran Ngan Thi My Luong Antimicrobial Effect of Platelet-Rich Plasma against Porphyromonas gingivalis International Journal of Dentistry |
| title | Antimicrobial Effect of Platelet-Rich Plasma against Porphyromonas gingivalis |
| title_full | Antimicrobial Effect of Platelet-Rich Plasma against Porphyromonas gingivalis |
| title_fullStr | Antimicrobial Effect of Platelet-Rich Plasma against Porphyromonas gingivalis |
| title_full_unstemmed | Antimicrobial Effect of Platelet-Rich Plasma against Porphyromonas gingivalis |
| title_short | Antimicrobial Effect of Platelet-Rich Plasma against Porphyromonas gingivalis |
| title_sort | antimicrobial effect of platelet rich plasma against porphyromonas gingivalis |
| url | http://dx.doi.org/10.1155/2019/7329103 |
| work_keys_str_mv | AT thuyanhvupham antimicrobialeffectofplateletrichplasmaagainstporphyromonasgingivalis AT thaothiphuongtran antimicrobialeffectofplateletrichplasmaagainstporphyromonasgingivalis AT nganthimyluong antimicrobialeffectofplateletrichplasmaagainstporphyromonasgingivalis |