Chitosan oligosaccharide decorated liposomes combined with TH302 for photodynamic therapy in triple negative breast cancer
Abstract Background Triple negative breast cancer (TNBC) is an aggressive tumor with extremely high mortality that results from its lack of effective therapeutic targets. As an adhesion molecule related to tumorigenesis and tumor metastasis, cluster of differentiation-44 (also known as CD44) is over...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-05-01
|
| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-021-00891-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849734639039545344 |
|---|---|
| author | Yinan Ding Rui Yang Weiping Yu Chunmei Hu Zhiyuan Zhang Dongfang Liu Yanli An Xihui Wang Chen He Peidang Liu Qiusha Tang Daozhen Chen |
| author_facet | Yinan Ding Rui Yang Weiping Yu Chunmei Hu Zhiyuan Zhang Dongfang Liu Yanli An Xihui Wang Chen He Peidang Liu Qiusha Tang Daozhen Chen |
| author_sort | Yinan Ding |
| collection | DOAJ |
| description | Abstract Background Triple negative breast cancer (TNBC) is an aggressive tumor with extremely high mortality that results from its lack of effective therapeutic targets. As an adhesion molecule related to tumorigenesis and tumor metastasis, cluster of differentiation-44 (also known as CD44) is overexpressed in TNBC. Moreover, CD44 can be effectively targeted by a specific hyaluronic acid analog, namely, chitosan oligosaccharide (CO). In this study, a CO-coated liposome was designed, with Photochlor (HPPH) as the 660 nm light mediated photosensitizer and evofosfamide (also known as TH302) as the hypoxia-activated prodrug. The obtained liposomes can help diagnose TNBC by fluorescence imaging and produce antitumor therapy by synergetic photodynamic therapy (PDT) and chemotherapy. Results Compared with the nontargeted liposomes, the targeted liposomes exhibited good biocompatibility and targeting capability in vitro; in vivo, the targeted liposomes exhibited much better fluorescence imaging capability. Additionally, liposomes loaded with HPPH and TH302 showed significantly better antitumor effects than the other monotherapy groups both in vitro and in vivo. Conclusion The impressive synergistic antitumor effects, together with the superior fluorescence imaging capability, good biocompatibility and minor side effects confers the liposomes with potential for future translational research in the diagnosis and CD44-overexpressing cancer therapy, especially TNBC. Graphic abstract |
| format | Article |
| id | doaj-art-fb8d47ef462c4fdfb7bbe63878c58ca3 |
| institution | DOAJ |
| issn | 1477-3155 |
| language | English |
| publishDate | 2021-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-fb8d47ef462c4fdfb7bbe63878c58ca32025-08-20T03:07:44ZengBMCJournal of Nanobiotechnology1477-31552021-05-0119111710.1186/s12951-021-00891-8Chitosan oligosaccharide decorated liposomes combined with TH302 for photodynamic therapy in triple negative breast cancerYinan Ding0Rui Yang1Weiping Yu2Chunmei Hu3Zhiyuan Zhang4Dongfang Liu5Yanli An6Xihui Wang7Chen He8Peidang Liu9Qiusha Tang10Daozhen Chen11Medical School of Southeast UniversityResearch Institute for Reproductive Health and Genetic Diseases, The Affiliated Wuxi Maternity and Child Health Care Hospital of Nanjing Medical UniversityMedical School of Southeast UniversityDepartment of Tuberculosis, The Second Affiliated Hospital of Southeast University (The Second Hospital of Nanjing)Department of Neurosurgery, Nanjing Jinling Hospital, Nanjing UniversityMedical School of Southeast UniversityAffiliated Zhongda Hospital of Southeast UniversityMedical School of Southeast UniversityMedical School of Southeast UniversityMedical School of Southeast UniversityMedical School of Southeast UniversityResearch Institute for Reproductive Health and Genetic Diseases, The Affiliated Wuxi Maternity and Child Health Care Hospital of Nanjing Medical UniversityAbstract Background Triple negative breast cancer (TNBC) is an aggressive tumor with extremely high mortality that results from its lack of effective therapeutic targets. As an adhesion molecule related to tumorigenesis and tumor metastasis, cluster of differentiation-44 (also known as CD44) is overexpressed in TNBC. Moreover, CD44 can be effectively targeted by a specific hyaluronic acid analog, namely, chitosan oligosaccharide (CO). In this study, a CO-coated liposome was designed, with Photochlor (HPPH) as the 660 nm light mediated photosensitizer and evofosfamide (also known as TH302) as the hypoxia-activated prodrug. The obtained liposomes can help diagnose TNBC by fluorescence imaging and produce antitumor therapy by synergetic photodynamic therapy (PDT) and chemotherapy. Results Compared with the nontargeted liposomes, the targeted liposomes exhibited good biocompatibility and targeting capability in vitro; in vivo, the targeted liposomes exhibited much better fluorescence imaging capability. Additionally, liposomes loaded with HPPH and TH302 showed significantly better antitumor effects than the other monotherapy groups both in vitro and in vivo. Conclusion The impressive synergistic antitumor effects, together with the superior fluorescence imaging capability, good biocompatibility and minor side effects confers the liposomes with potential for future translational research in the diagnosis and CD44-overexpressing cancer therapy, especially TNBC. Graphic abstracthttps://doi.org/10.1186/s12951-021-00891-8Triple negative breast cancerPhotodynamic therapyChitosan oligosaccharideCD44Liposomes |
| spellingShingle | Yinan Ding Rui Yang Weiping Yu Chunmei Hu Zhiyuan Zhang Dongfang Liu Yanli An Xihui Wang Chen He Peidang Liu Qiusha Tang Daozhen Chen Chitosan oligosaccharide decorated liposomes combined with TH302 for photodynamic therapy in triple negative breast cancer Journal of Nanobiotechnology Triple negative breast cancer Photodynamic therapy Chitosan oligosaccharide CD44 Liposomes |
| title | Chitosan oligosaccharide decorated liposomes combined with TH302 for photodynamic therapy in triple negative breast cancer |
| title_full | Chitosan oligosaccharide decorated liposomes combined with TH302 for photodynamic therapy in triple negative breast cancer |
| title_fullStr | Chitosan oligosaccharide decorated liposomes combined with TH302 for photodynamic therapy in triple negative breast cancer |
| title_full_unstemmed | Chitosan oligosaccharide decorated liposomes combined with TH302 for photodynamic therapy in triple negative breast cancer |
| title_short | Chitosan oligosaccharide decorated liposomes combined with TH302 for photodynamic therapy in triple negative breast cancer |
| title_sort | chitosan oligosaccharide decorated liposomes combined with th302 for photodynamic therapy in triple negative breast cancer |
| topic | Triple negative breast cancer Photodynamic therapy Chitosan oligosaccharide CD44 Liposomes |
| url | https://doi.org/10.1186/s12951-021-00891-8 |
| work_keys_str_mv | AT yinanding chitosanoligosaccharidedecoratedliposomescombinedwithth302forphotodynamictherapyintriplenegativebreastcancer AT ruiyang chitosanoligosaccharidedecoratedliposomescombinedwithth302forphotodynamictherapyintriplenegativebreastcancer AT weipingyu chitosanoligosaccharidedecoratedliposomescombinedwithth302forphotodynamictherapyintriplenegativebreastcancer AT chunmeihu chitosanoligosaccharidedecoratedliposomescombinedwithth302forphotodynamictherapyintriplenegativebreastcancer AT zhiyuanzhang chitosanoligosaccharidedecoratedliposomescombinedwithth302forphotodynamictherapyintriplenegativebreastcancer AT dongfangliu chitosanoligosaccharidedecoratedliposomescombinedwithth302forphotodynamictherapyintriplenegativebreastcancer AT yanlian chitosanoligosaccharidedecoratedliposomescombinedwithth302forphotodynamictherapyintriplenegativebreastcancer AT xihuiwang chitosanoligosaccharidedecoratedliposomescombinedwithth302forphotodynamictherapyintriplenegativebreastcancer AT chenhe chitosanoligosaccharidedecoratedliposomescombinedwithth302forphotodynamictherapyintriplenegativebreastcancer AT peidangliu chitosanoligosaccharidedecoratedliposomescombinedwithth302forphotodynamictherapyintriplenegativebreastcancer AT qiushatang chitosanoligosaccharidedecoratedliposomescombinedwithth302forphotodynamictherapyintriplenegativebreastcancer AT daozhenchen chitosanoligosaccharidedecoratedliposomescombinedwithth302forphotodynamictherapyintriplenegativebreastcancer |