Urinary Proteome Analysis Identified Neprilysin and VCAM as Proteins Involved in Diabetic Nephropathy
Urinary proteome was analyzed and quantified by tandem mass tag (TMT) labeling followed by bioinformatics analysis to study diabetic nephropathy (DN) pathophysiology and to identify biomarkers of a clinical outcome. We included type 2 diabetic normotensive non-obese males with (n=9) and without (n=1...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2018/6165303 |
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| author | Elena Guillén-Gómez Beatriz Bardají-de-Quixano Sílvia Ferrer Carlos Brotons Mark A. Knepper Montserrat Carrascal Joaquin Abian José M. Mas Francesca Calero José A. Ballarín Patricia Fernández-Llama |
| author_facet | Elena Guillén-Gómez Beatriz Bardají-de-Quixano Sílvia Ferrer Carlos Brotons Mark A. Knepper Montserrat Carrascal Joaquin Abian José M. Mas Francesca Calero José A. Ballarín Patricia Fernández-Llama |
| author_sort | Elena Guillén-Gómez |
| collection | DOAJ |
| description | Urinary proteome was analyzed and quantified by tandem mass tag (TMT) labeling followed by bioinformatics analysis to study diabetic nephropathy (DN) pathophysiology and to identify biomarkers of a clinical outcome. We included type 2 diabetic normotensive non-obese males with (n=9) and without (n=11) incipient DN (microalbuminuria). Sample collection included blood and urine at baseline (control and DN basal) and, in DN patients, after 3 months of losartan treatment (DN treated). Urinary proteome analysis identified 166 differentially abundant proteins between controls and DN patients, 27 comparing DN-treated and DN-basal patients, and 182 between DN-treated patients and controls. The mathematical modeling analysis predicted 80 key proteins involved in DN pathophysiology and 15 in losartan effect, a total of 95 proteins. Out of these 95, 7 are involved in both processes. VCAM-1 and neprilysin stand out of these 7 for being differentially expressed in the urinary proteome. We observed an increase of VCAM-1 urine levels in DN-basal patients compared to diabetic controls and an increase of urinary neprilysin in DN-treated patients with persistent albuminuria; the latter was confirmed by ELISA. Our results point to neprilysin and VCAM-1 as potential candidates in DN pathology and treatment. |
| format | Article |
| id | doaj-art-fb86f29967484d1fad3ba5d1a8157e5f |
| institution | OA Journals |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-fb86f29967484d1fad3ba5d1a8157e5f2025-08-20T02:22:56ZengWileyJournal of Diabetes Research2314-67452314-67532018-01-01201810.1155/2018/61653036165303Urinary Proteome Analysis Identified Neprilysin and VCAM as Proteins Involved in Diabetic NephropathyElena Guillén-Gómez0Beatriz Bardají-de-Quixano1Sílvia Ferrer2Carlos Brotons3Mark A. Knepper4Montserrat Carrascal5Joaquin Abian6José M. Mas7Francesca Calero8José A. Ballarín9Patricia Fernández-Llama10Molecular Biology Laboratory, Fundació Puigvert, Barcelona, SpainUniversitat Autònoma de Barcelona, Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau), Barcelona, SpainMaragall Primary Health Care Center, Barcelona, SpainUniversitat Autònoma de Barcelona, Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau), Barcelona, SpainEpithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute (NIH), Bethesda, MD, USAProteomics Laboratory CSIC/UAB, Institut d’Investigacions Biomèdiques de Barcelona (IIBB-CSIC-IDIBAPS), Barcelona, SpainProteomics Laboratory CSIC/UAB, Institut d’Investigacions Biomèdiques de Barcelona (IIBB-CSIC-IDIBAPS), Barcelona, SpainAnaxomics Biotech SL, Barcelona, SpainUniversitat Autònoma de Barcelona, Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau), Barcelona, SpainUniversitat Autònoma de Barcelona, Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau), Barcelona, SpainUniversitat Autònoma de Barcelona, Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau), Barcelona, SpainUrinary proteome was analyzed and quantified by tandem mass tag (TMT) labeling followed by bioinformatics analysis to study diabetic nephropathy (DN) pathophysiology and to identify biomarkers of a clinical outcome. We included type 2 diabetic normotensive non-obese males with (n=9) and without (n=11) incipient DN (microalbuminuria). Sample collection included blood and urine at baseline (control and DN basal) and, in DN patients, after 3 months of losartan treatment (DN treated). Urinary proteome analysis identified 166 differentially abundant proteins between controls and DN patients, 27 comparing DN-treated and DN-basal patients, and 182 between DN-treated patients and controls. The mathematical modeling analysis predicted 80 key proteins involved in DN pathophysiology and 15 in losartan effect, a total of 95 proteins. Out of these 95, 7 are involved in both processes. VCAM-1 and neprilysin stand out of these 7 for being differentially expressed in the urinary proteome. We observed an increase of VCAM-1 urine levels in DN-basal patients compared to diabetic controls and an increase of urinary neprilysin in DN-treated patients with persistent albuminuria; the latter was confirmed by ELISA. Our results point to neprilysin and VCAM-1 as potential candidates in DN pathology and treatment.http://dx.doi.org/10.1155/2018/6165303 |
| spellingShingle | Elena Guillén-Gómez Beatriz Bardají-de-Quixano Sílvia Ferrer Carlos Brotons Mark A. Knepper Montserrat Carrascal Joaquin Abian José M. Mas Francesca Calero José A. Ballarín Patricia Fernández-Llama Urinary Proteome Analysis Identified Neprilysin and VCAM as Proteins Involved in Diabetic Nephropathy Journal of Diabetes Research |
| title | Urinary Proteome Analysis Identified Neprilysin and VCAM as Proteins Involved in Diabetic Nephropathy |
| title_full | Urinary Proteome Analysis Identified Neprilysin and VCAM as Proteins Involved in Diabetic Nephropathy |
| title_fullStr | Urinary Proteome Analysis Identified Neprilysin and VCAM as Proteins Involved in Diabetic Nephropathy |
| title_full_unstemmed | Urinary Proteome Analysis Identified Neprilysin and VCAM as Proteins Involved in Diabetic Nephropathy |
| title_short | Urinary Proteome Analysis Identified Neprilysin and VCAM as Proteins Involved in Diabetic Nephropathy |
| title_sort | urinary proteome analysis identified neprilysin and vcam as proteins involved in diabetic nephropathy |
| url | http://dx.doi.org/10.1155/2018/6165303 |
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