Biochemical Study of Induced Diabetes Mellitus by Experimental Total Pancreatectomy in Dogs

The objective of the current study was evaluating the status of blood serum biochemical parameters in poorly controlled diabetes mellitus induced after experimental total pancreatectomy in local crossbreeding dogs. The biochemical assay was taken before the surgical operation and on the last two day...

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Main Authors: Nadhim Hasan, Borhan Al-Mufti.
Format: Article
Language:English
Published: College of Veterinary Medicine, University of Basrah, Iraq 2023-06-01
Series:Basrah Journal of Veterinary Research
Subjects:
Online Access:https://bjvr.uobasrah.edu.iq/article_179919_7bf3521956ebafa39b9514626794e366.pdf
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author Nadhim Hasan
Borhan Al-Mufti.
author_facet Nadhim Hasan
Borhan Al-Mufti.
author_sort Nadhim Hasan
collection DOAJ
description The objective of the current study was evaluating the status of blood serum biochemical parameters in poorly controlled diabetes mellitus induced after experimental total pancreatectomy in local crossbreeding dogs. The biochemical assay was taken before the surgical operation and on the last two days of the insulin treatment period and after termination of insulin therapy till death of animals due to ketoacidosis after detecting severe ketonuria on urinalysis by urine stripe test when insulin withdrawn. Results of estimating the various biochemical parameters revealed a significant increase in the blood serum level of liver enzymes [Alanine amino transaminase, alkaline phosphatase, and Aspartate amino transaminase], total cholesterol and potassium ions, while a significant decrease in chloride ions took place in both insulin treatment and withdrawal periods. On the other hand, significant increases in blood serum levels of triglyceride, total bilirubin, sodium ions, and blood urea nitrogen with significant decrease in total calcium was occurred in the insulin withdrawal period only. In conclusion, the first mentioned biochemical parameters might be involved in the pathogenesis of poorly controlled diabetes mellitus while the later mentioned parameters which exhibited significant changes only after cessation of insulin therapy might be involved in the pathogenesis of surgically induced diabetic ketoacidosis in dogs.
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spelling doaj-art-fb76745d22d64b0ebd494e5722afe54b2025-08-20T02:30:55ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562023-06-01222122310.23975/bjvetr.2023.179919179919Biochemical Study of Induced Diabetes Mellitus by Experimental Total Pancreatectomy in DogsNadhim Hasan0Borhan Al-Mufti.1Department of Medicine& Surgery, College of Veterinary Medicine, University of Duhok-Iraq.Department of Medicine& Surgery, College of Veterinary Medicine, University of Duhok-Iraq.The objective of the current study was evaluating the status of blood serum biochemical parameters in poorly controlled diabetes mellitus induced after experimental total pancreatectomy in local crossbreeding dogs. The biochemical assay was taken before the surgical operation and on the last two days of the insulin treatment period and after termination of insulin therapy till death of animals due to ketoacidosis after detecting severe ketonuria on urinalysis by urine stripe test when insulin withdrawn. Results of estimating the various biochemical parameters revealed a significant increase in the blood serum level of liver enzymes [Alanine amino transaminase, alkaline phosphatase, and Aspartate amino transaminase], total cholesterol and potassium ions, while a significant decrease in chloride ions took place in both insulin treatment and withdrawal periods. On the other hand, significant increases in blood serum levels of triglyceride, total bilirubin, sodium ions, and blood urea nitrogen with significant decrease in total calcium was occurred in the insulin withdrawal period only. In conclusion, the first mentioned biochemical parameters might be involved in the pathogenesis of poorly controlled diabetes mellitus while the later mentioned parameters which exhibited significant changes only after cessation of insulin therapy might be involved in the pathogenesis of surgically induced diabetic ketoacidosis in dogs.https://bjvr.uobasrah.edu.iq/article_179919_7bf3521956ebafa39b9514626794e366.pdfdiabetespancreatectomyparametersketoacidosis
spellingShingle Nadhim Hasan
Borhan Al-Mufti.
Biochemical Study of Induced Diabetes Mellitus by Experimental Total Pancreatectomy in Dogs
Basrah Journal of Veterinary Research
diabetes
pancreatectomy
parameters
ketoacidosis
title Biochemical Study of Induced Diabetes Mellitus by Experimental Total Pancreatectomy in Dogs
title_full Biochemical Study of Induced Diabetes Mellitus by Experimental Total Pancreatectomy in Dogs
title_fullStr Biochemical Study of Induced Diabetes Mellitus by Experimental Total Pancreatectomy in Dogs
title_full_unstemmed Biochemical Study of Induced Diabetes Mellitus by Experimental Total Pancreatectomy in Dogs
title_short Biochemical Study of Induced Diabetes Mellitus by Experimental Total Pancreatectomy in Dogs
title_sort biochemical study of induced diabetes mellitus by experimental total pancreatectomy in dogs
topic diabetes
pancreatectomy
parameters
ketoacidosis
url https://bjvr.uobasrah.edu.iq/article_179919_7bf3521956ebafa39b9514626794e366.pdf
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AT borhanalmufti biochemicalstudyofinduceddiabetesmellitusbyexperimentaltotalpancreatectomyindogs