An Exploration of the Impact of Anticentromere Antibody on Early-Stage Embryo
Background. Previously, we found women with positive anticentromere antibody showed impaired potential of oocyte maturation and embryo cleavage; the possible mechanism behind this phenomenon was still unknown. Objective. Thus, the present study aimed to preliminarily explore whether ACA could penetr...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2017/4809294 |
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| author | Ying Ying Xi Guo Yiping Zhong Canquan Zhou |
| author_facet | Ying Ying Xi Guo Yiping Zhong Canquan Zhou |
| author_sort | Ying Ying |
| collection | DOAJ |
| description | Background. Previously, we found women with positive anticentromere antibody showed impaired potential of oocyte maturation and embryo cleavage; the possible mechanism behind this phenomenon was still unknown. Objective. Thus, the present study aimed to preliminarily explore whether ACA could penetrate into the living embryos and impair their developmental potential via in vitro coculture with mouse embryos. Methods. Mouse embryos were collected and used for in vitro culture with polyclonal anticentromere protein A (CENP-A) antibody; then, immunofluorescence assay was performed to determine the penetration of antibody into embryos, and embryo development potential was observed. Results. All embryos cultured with anti-CENP-A antibody exhibited immunofluorescence on the nucleus, while none of the embryos from the control groups showed immunofluorescence. Additionally, embryos cultured with anti-CENP-A antibody experienced significant growth impairment compared with controls. Conclusion. Mouse embryos may be a direct target for ACA in vitro prior to implantation. However, the precise mechanism needs further clarification. |
| format | Article |
| id | doaj-art-fb72a02eb4304e55b5fac8e72eff115a |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-fb72a02eb4304e55b5fac8e72eff115a2025-08-20T03:25:31ZengWileyJournal of Immunology Research2314-88612314-71562017-01-01201710.1155/2017/48092944809294An Exploration of the Impact of Anticentromere Antibody on Early-Stage EmbryoYing Ying0Xi Guo1Yiping Zhong2Canquan Zhou3Reproductive Medicine Center, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaReproductive Medicine Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaReproductive Medicine Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaReproductive Medicine Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaBackground. Previously, we found women with positive anticentromere antibody showed impaired potential of oocyte maturation and embryo cleavage; the possible mechanism behind this phenomenon was still unknown. Objective. Thus, the present study aimed to preliminarily explore whether ACA could penetrate into the living embryos and impair their developmental potential via in vitro coculture with mouse embryos. Methods. Mouse embryos were collected and used for in vitro culture with polyclonal anticentromere protein A (CENP-A) antibody; then, immunofluorescence assay was performed to determine the penetration of antibody into embryos, and embryo development potential was observed. Results. All embryos cultured with anti-CENP-A antibody exhibited immunofluorescence on the nucleus, while none of the embryos from the control groups showed immunofluorescence. Additionally, embryos cultured with anti-CENP-A antibody experienced significant growth impairment compared with controls. Conclusion. Mouse embryos may be a direct target for ACA in vitro prior to implantation. However, the precise mechanism needs further clarification.http://dx.doi.org/10.1155/2017/4809294 |
| spellingShingle | Ying Ying Xi Guo Yiping Zhong Canquan Zhou An Exploration of the Impact of Anticentromere Antibody on Early-Stage Embryo Journal of Immunology Research |
| title | An Exploration of the Impact of Anticentromere Antibody on Early-Stage Embryo |
| title_full | An Exploration of the Impact of Anticentromere Antibody on Early-Stage Embryo |
| title_fullStr | An Exploration of the Impact of Anticentromere Antibody on Early-Stage Embryo |
| title_full_unstemmed | An Exploration of the Impact of Anticentromere Antibody on Early-Stage Embryo |
| title_short | An Exploration of the Impact of Anticentromere Antibody on Early-Stage Embryo |
| title_sort | exploration of the impact of anticentromere antibody on early stage embryo |
| url | http://dx.doi.org/10.1155/2017/4809294 |
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